NCT05782244

Brief Summary

Traumatic Cerebral Vascular Injury (TCVI) is a common consequence of traumatic brain injury (TBI), including mild TBI (mTBI). TCVI is associated with poor recovery after TBI in animal models. TCVI can be measured non-invasively in humans, and therapies targeting TCVI are attractive candidates to ameliorate the consequences of TBI. Sildenafil potentiates nitric oxide (NO) dependent vasodilatation and is approved by the Food and Drug Administration (FDA) for the treatment of erectile dysfunction and primary pulmonary hypertension. In pre-clinical models of stroke, sildenafil improves cerebral blood flow (CBF), promotes, angiogenesis, neurogenesis and improves recovery. In an initial Phase 2a trial (NCT01762475) of sildenafil in patients with chronic moderate to severe TBI, the investigators found that low dose sildenafil (25 mg BID) therapy is safe and well tolerated, that a single dose of sildenafil 50 mg potentiates CVR in areas of the brain with dysfunctional endothelium, and that CVR is a reliable diagnostic marker of TCVI and has potential as a pharmacodynamic and predictive biomarker. In this proposal, the investigators will conduct a randomized clinical trial to determine the optimal PDE5 inhibitor dose to improve or normalize microvascular function (as measured by the change in CVR measurements before and after a single dose of sildenafil, or ΔCVR) using a range of sildenafil citrate doses: 20, 40, 80 mg) in chronic TBI patients. The investigators will also test the safety and tolerability of the same dose ranges of chronic (4-week) thrice daily sildenafil or placebo administration in chronic TBI patients and explore its effects on chronic symptoms and clinical outcomes.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
160

participants targeted

Target at P75+ for phase_2

Timeline
20mo left

Started Jul 2024

Typical duration for phase_2

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress54%
Jul 2024Dec 2027

First Submitted

Initial submission to the registry

November 22, 2022

Completed
4 months until next milestone

First Posted

Study publicly available on registry

March 23, 2023

Completed
1.3 years until next milestone

Study Start

First participant enrolled

July 2, 2024

Completed
3.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2027

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2027

Last Updated

December 10, 2025

Status Verified

December 1, 2025

Enrollment Period

3.5 years

First QC Date

November 22, 2022

Last Update Submit

December 3, 2025

Conditions

Outcome Measures

Primary Outcomes (1)

  • Optimal Dose

    Delta cerebrovascular reactivity (ΔCVR) after single-dose sildenafil administration during the neuroimaging session.

    Assessed at baseline visit

Secondary Outcomes (1)

  • Number of participants with treatment-related adverse events as assessed by CTCAE v4.0

    Assessed over 4 week daily intervention of 3 doses of sildenafil in TBI patients

Other Outcomes (2)

  • Clinician Interview-Based Impression (CIBI)

    Assessed at baseline and at the end of the 4 week intervention.

  • Glasgow Outcome Scale-Extended (GOSE)

    Assessed at baseline and at the end of the 4 week intervention.

Study Arms (4)

Sildenafil citrate low dose

ACTIVE COMPARATOR

Sildenafil citrate 20 mg, oral, TID

Drug: Sildenafil Citrate low dose

Placebo

PLACEBO COMPARATOR

Placebo, oral, TID

Drug: Placebo

Sildenafil citrate medium dose

ACTIVE COMPARATOR

Sildenafil citrate 40 mg, oral, TID

Drug: Sildenafil medium dose

Sildenafil citrate high dose

ACTIVE COMPARATOR

Sildenafil citrate 80 mg, oral, TID

Drug: Sildenafil high dose

Interventions

Sildenafil 40 mg, oral, TID

Also known as: Sildenafil middle dose
Sildenafil citrate medium dose

Sildenafil 80 mg, oral, TID

Also known as: Sildenafil highest dose
Sildenafil citrate high dose

Sildenafil citrate 20 mg, oral, TID

Also known as: Sildenafil lowest dose
Sildenafil citrate low dose

Placebo, oral, TID

Also known as: Masked placebo
Placebo

Eligibility Criteria

Age18 Years - 70 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • To be eligible to participate in this study, an individual must meet all of the following criteria:
  • Provision of signed and dated informed consent form
  • Stated willingness to comply with all study procedures and availability for the duration of the study
  • Male or female, aged 18-70
  • Defense Enrollment Eligibility Reporting System (DEERS) eligible (WRNMMC only; N/A for UPenn)
  • History of TBI greater than 6 months and less than 25 years prior to enrollment, based on the 2023 American Congress of Rehabilitation Medicine Criteria92;
  • Plausible Mechanism of Injury: Criterion 1 focuses on the external force that likely disrupted brain function, including blast/explosion forces and acknowledging intentional causes.
  • Clinical Signs: Criterion 2 highlights signs observed or elicited during examination, distinguishing them from subjective symptoms.
  • Acute Symptoms: Criterion 3 considers subjective acute symptoms, requiring at least two symptoms to be present.
  • Clinical Examination/Laboratory Findings: Criterion 4 is a new addition, incorporating findings like cognitive impairment or oculomotor impairment.
  • Neuroimaging: While not required for diagnosis, neuroimaging can be used to provide further evidence of brain injury.
  • Chronic persistent post-concussive symptoms (Symptom Score \> 1 on at least 3 items from the Rivermead Post-Concussion Symptom Questionnaire; RPQ)
  • Glasgow Outcome Scale-Extended (GOSE) between 5-7
  • Ability to take oral medication and be willing to adhere to the study intervention regimen
  • Ability to participate in and complete 1 MRI including 2 CVR challenges and 4-week treatment period.
  • +1 more criteria

You may not qualify if:

  • An individual who meets any of the following criteria will be excluded from participation in this study:
  • Contraindication to sildenafil which includes the following:
  • Current/ongoing (within past month) use of organic nitrate vasodilators or riociguat
  • Current/ongoing (within past month) use of ritonavir (HIV-protease inhibitor)
  • Current/ongoing (within past month) use of erythromycin, ketoconazole, or itraconazole
  • Current/ongoing (within past month) use of cimetidine
  • Current resting hypotension (BP \< 90/50 mm Hg)
  • Current severe renal insufficiency
  • Current hepatic cirrhosis
  • Current cardiac failure or coronary artery disease causing unstable angina
  • Retinitis pigmentosa
  • Known hypersensitivity or allergy to sildenafil of any of its components
  • History of melanoma or suspicious skin lesions for melanoma on skin examination
  • Daily therapy with a PDE5 inhibitor within the month prior to consent
  • History of penetrating TBI
  • +11 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Pennsylvania

Philadelphia, Pennsylvania, 19104, United States

RECRUITING

Related Publications (1)

  • Kalyani P, Lippa SM, Werner JK, Amyot F, Moore CB, Kenney K, Diaz-Arrastia R. Phosphodiesterase-5 (PDE-5) Inhibitors as Therapy for Cerebrovascular Dysfunction in Chronic Traumatic Brain Injury. Neurotherapeutics. 2023 Oct;20(6):1629-1640. doi: 10.1007/s13311-023-01430-z. Epub 2023 Sep 11.

MeSH Terms

Conditions

Brain Injuries, Traumatic

Interventions

Sildenafil Citrate

Condition Hierarchy (Ancestors)

Brain InjuriesBrain DiseasesCentral Nervous System DiseasesNervous System DiseasesCraniocerebral TraumaTrauma, Nervous SystemWounds and Injuries

Intervention Hierarchy (Ancestors)

SulfonamidesAmidesOrganic ChemicalsSulfonesSulfur CompoundsPiperazinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsPurinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-Ring

Study Officials

  • Ramon Diaz-Arrastia, MD, PhD

    University of Pennsylvania

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
SEQUENTIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 22, 2022

First Posted

March 23, 2023

Study Start

July 2, 2024

Primary Completion (Estimated)

December 31, 2027

Study Completion (Estimated)

December 31, 2027

Last Updated

December 10, 2025

Record last verified: 2025-12

Locations