Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults

NCT05760066 | Unknown

• 1 other identifier: MDR0823
• Study Type: interventional
• Target: 24
• Locations: 0 countries
• Sites: N/A

Brief Summary

The goal of this clinical trial is to compare the effects of resistance training (RT) preconditioning vs no training on disuse-induced atrophy and post-disuse resistance training in young healthy individuals. The main questions it aims to answer are:

• To determine if performing RT prior to a period of disuse enhances the regain of strength, skeletal muscle size, and skeletal muscle quality while performing RT after a period of disuse.
• To determine if performing RT prior to a period of disuse dampens the maladaptive effects of disuse on muscle size, muscle quality, and strength.
• To determine the anabolic and proteolytic mechanisms underpinning the observed outcomes. Participants will:
• Perform either 6 weeks of resistance training or maintain an untrained lifestyle
• Perform 2 weeks of limb immobilization induced disuse of a randomized leg
• Perform 6 weeks of resistance training

Conditions

Atrophy, Disuse; Atrophy, Muscular

Outcome Measures

Primary Outcomes (3)

• Change in vastus lateralis fiber cross-sectional area

  Upon the procurement and analysis of muscle from the vastus lateralis of participants, histological techniques will be used to assess fiber cross-sectional area of muscle fibers

  Through completion of study, 16 weeks

• Change in quadriceps strength via 3 repetition maximum testing

  Strength of the quadriceps will be assessed via 3-repetition maximum testing of select exercises (leg press, leg extension, hex-bar deadlift)

  Through completion of study, 16 weeks

• Change in quadriceps strength via isokinetic dynamometry

  Strength of the quadriceps will be assessed via isokinetic dynamometry

  Through completion of study, 16 weeks

Secondary Outcomes (4)

• Change in protein expression of anabolic signaling proteins

  Through completion of study, 16 weeks

• Change in protein expression of catabolic signaling proteins

  Through completion of study, 16 weeks

• Change in activity of catabolic systems

  Through completion of study, 16 weeks

• Gene expression at the mRNA level of atrophy-associated genes (atrogenes)

  Through completion of study, 16 weeks

Study Arms (2)

Resistance Training Preconditioning (PRECON)

EXPERIMENTAL

This group will perform: 1. 6 weeks of lower body focused resistance training 2. 2 weeks of locking brace-induced immobilization of a randomized leg 3. 6 weeks of lower body focused resistance training

Other: Resistance Training Preconditioning

Control (CTL)

ACTIVE COMPARATOR

This group will perform: 1. 6 weeks of activities of daily living (no training) 2. 2 weeks of locking brace-induced immobilization of a randomized leg 3. 6 weeks of lower body focused resistance training

Other: Control

Interventions

Resistance Training Preconditioning OTHER

Resistance training prior to disuse-induced atrophy followed by another period of resistance training

Resistance Training Preconditioning (PRECON)

Control OTHER

Activities of daily living prior to disuse-induced atrophy followed by a period of resistance training

Control (CTL)

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Between the ages of 18-30
• Free from medical implants in lower extremity that would interfere with low-dose radiation
• Are not allergic to ultrasound gel or Lidocaine
• Have no or minimal experience with resistance training (less than 1 training session/week for a 1 year period)

You may not qualify if:

• Have been exposed to medically necessary radiation in the past 12 months other than dental x-rays
• Have known overt cardiovascular or metabolic disease
• Have a medical condition that would contraindicate participating in an exercise program or donating a skeletal muscle biopsy (e.g. blood clotting disorder, taking blood thinners, etc.)
• Are undergoing medical treatment for a disease that would interfere physiologically or logistically with study outcomes and/or protocols (e.g. undergoing chemotherapy and/or radiation for cancer treatment)
• Have consumed supplemental protein, creatine, and/or agents that affect hormone status (e.g. testosterone, growth hormone boosters, etc.) within the previous 2 months.

Sponsors & Collaborators

• Auburn University: lead

MeSH Terms

Conditions

Muscular Disorders, Atrophic; Muscular Atrophy

Condition Hierarchy (Ancestors)

Muscular Diseases; Musculoskeletal Diseases; Neuromuscular Diseases; Nervous System Diseases; Neuromuscular Manifestations; Neurologic Manifestations; Atrophy; Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Signs and Symptoms

Central Study Contacts

Michael D Roberts, PhD

CONTACT

334-844-1925; mdr0024@auburn.edu

John M Michel, MS

CONTACT

2059077830; michel@auburn.edu

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: January 23, 2023
• First Posted: March 8, 2023
• Study Start: August 1, 2023
• Primary Completion: December 31, 2023
• Study Completion: August 1, 2024
• Last Updated: March 8, 2023

Record last verified: 2023-02