Phenotypic and Genotypic Variations of Enterococcus Spp
1 other identifier
observational
100
1 country
1
Brief Summary
Enterococci are Gram-positive facultative anaerobic cocci arranged in short and medium chains. Enterococci reside in the gastrointestinal tract and usually function commensally with humans. They can, however, cause several infections, such as urinary tract infections (UTIs), intra-abdominal infection, bacteremia, or endocarditis. Among many species identified, E. faecalis and E. faecium are the most common species capable of causing infection and posing a threat of antimicrobial resistance, with E. faecalis accounting for the majority of infections.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P50-P75 for all trials
Started Apr 2023
Shorter than P25 for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
Click on a node to explore related trials.
Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
February 20, 2023
CompletedFirst Posted
Study publicly available on registry
March 2, 2023
CompletedStudy Start
First participant enrolled
April 1, 2023
CompletedPrimary Completion
Last participant's last visit for primary outcome
December 31, 2023
CompletedStudy Completion
Last participant's last visit for all outcomes
March 30, 2024
CompletedApril 19, 2023
April 1, 2023
9 months
February 20, 2023
April 16, 2023
Conditions
Keywords
Outcome Measures
Primary Outcomes (6)
Isolation and identification of Enterococci from different clinical samples
Isolation and identification of Enterococci from different clinical samples (pus, urine, urinary catheter samples, stool, wound swabs, sputum, central venous catheter samples) will be collected under aseptic precautions from patients admitted at different departments at Sohag university hospitals.
from April 2023 to December 2023
Demonstration of Enterococci antibiotic susceptibility profile
Strains confirmed as Enterococci will be examined for their antibiotic susceptibility by modified Kirby Bauer's disc diffusion method on Mueller-Hinton Agar.
from April 2023 to December 2023
Detection of strains of Enterococci which produce biofilm
The biofilm formation activity of Enterococci isolates will be tested using the microtiter plate technique
from April 2023 to December 2023
Studying the effect of nanoparticles on Enterococci antimicrobial resistance pattern
Inhibition zones of all groups will be compared to detect the effect of nanoparticles on Enterococci antimicrobial resistance pattern.
from April 2023 to December 2023
Studying the effect of nanoparticles on Enterococci biofilm producing ability
The effect of nanoparticles on Enterococci biofilm producing ability
from December 2023 to March 2024
Genotypic characterization of Enterococci.
Molecular identification of some virulence factors genes and antibiotic resistance genes of Enterococci using polymerase chain reaction.
rom December 2023 to March 2024
Study Arms (2)
samples containing Enterococci
Isolates of Enterococci will be identified by Gram staining, colony morphology, catalase test, and growth on Bile Esculin agar. All isolates will be identified to species level using Vitek2 automated system Strains confirmed as Enterococci will be examined for their antibiotic susceptibility by modified Kirby Bauer's disc diffusion method on MuellerHinton Agar. The biofilm formation activity of Enterococci isolates will be tested using the microtiter plate technique Detection of the effect of nanoparticles on the antibiotic susceptibility profile of Enterococci. Detection of the effect of nanoparticles on the biofilm producing Enterococci. Molecular identification of some virulence factors genes and antibiotic resistance genes of Enterococci using PCR
samples with bacteria other than enterococci
Interventions
* Samples will be transported to Medical Microbiology and Immunology Laboratory and will be inoculated on MacConkey medium. * Pink colonies will be inoculated on bile esculin agar medium. * Enterococci produce blackening of the agar
The growing colonies on bile esculin agar medium will be examined microscopically after staining by Gram stain.
* Catalase test. * Salt tolerance test
Automated identification of Enterococci will be done with VITEK2 system.
Strains confirmed as Enterococci will be examined for their antibiotic susceptibility by modified Kirby Bauer's disc diffusion method on Mueller-Hinton Agar.
The effect of nanoparticles on Enterococci antimicrobial resistance pattern
The biofilm formation activity of Enterococci isolates will be tested using the microtiter plate technique
Molecular identification of some virulence factors genes and antibiotic resistance genes of Enterococci using PCR
Eligibility Criteria
All the selected patients will be subjected to complete history taking (personal, clinical, associated comorbidities, history of drug intake, and length of hospital stay before sampling). \* Collected samples will be transported to the Medical Microbiology and Immunology research laboratory as soon as possible.
You may qualify if:
- All patients suffering from infections that can be caused by Enterococci.
You may not qualify if:
- All patients suffering from infections that aren't caused by Enterococci
Contact the study team to confirm eligibility.
Sponsors & Collaborators
- Sohag Universitylead
Study Sites (1)
Faculty Of Medicine
Sohag, 82524, Egypt
Related Publications (1)
Ferguson DM, Talavera GN, Hernandez LA, Weisberg SB, Ambrose RF, Jay JA. Virulence Genes among Enterococcus faecalis and Enterococcus faecium Isolated from Coastal Beaches and Human and Nonhuman Sources in Southern California and Puerto Rico. J Pathog. 2016;2016:3437214. doi: 10.1155/2016/3437214. Epub 2016 Apr 10.
PMID: 27144029BACKGROUND
Biospecimen
Different samples (pus, urine, urinary catheter samples, stool, wound swabs, sputum, central venous catheter samples) will be collected under aseptic precautions from patients admitted at different departments at Sohag university hospitals.
MeSH Terms
Interventions
Intervention Hierarchy (Ancestors)
Study Officials
- STUDY CHAIR
Mona F Mohamed, professor
Sohag University
- STUDY CHAIR
Wesam A Abu El wafa
Sohag University
Central Study Contacts
Study Design
- Study Type
- observational
- Observational Model
- CASE CONTROL
- Time Perspective
- CROSS SECTIONAL
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- principal investigator
Study Record Dates
First Submitted
February 20, 2023
First Posted
March 2, 2023
Study Start
April 1, 2023
Primary Completion
December 31, 2023
Study Completion
March 30, 2024
Last Updated
April 19, 2023
Record last verified: 2023-04
Data Sharing
- IPD Sharing
- Will not share