Auramine Phenol Staining Technique for Revealing Different Coccidian Parasites

NCT05724381 | Unknown

• 1 other identifier: Soh-Med-23-01-25
• Study Type: observational
• Target: 100
• Locations: 0 countries
• Sites: N/A

Brief Summary

The increased number of documented human coccidian infections, including Cryptosporidium parvum, Cyclospora cayetanensis, Isospora belli, and Sarcocystis spp., that are often indistinguishable from other forms of community-acquired diarrhea, together with the possibility of treating some of them, suggests a need for proper diagnostic techniques to recover and identify these organisms

Conditions

Chronic Diarrhea

Keywords

coccidian parasites; auramine phenol staining; fluorescence microscopy; modified Ziehl Nelseen; Cryptosporidium

Outcome Measures

Primary Outcomes (1)

• prevalence of coccidian parasites

  number of patients positive for coccidian parasites

  1 month

Secondary Outcomes (1)

• auramine phenol staining

  1 month

Interventions

auramine phenol staining DIAGNOSTIC_TEST

The samples will be divided into 2 parts. The 1st part will be subjected to a direct wet saline smear, iodine smear, and concentration techniques. The 2nd part will be used to perform thin smears of the fecal concentrates and air-dried on 2 different slides. The first slide will be stained with Kinyoun's acid-fast stain and examined with the conventional light microscope and the second will be stained with auramine-phenol stain and examined with the fluorescent microscope.

Eligibility Criteria

• Age: 6 Years - 70 Years
• Sex: all
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

patients from outpatient clinics in different hospitals in Sohag governorate complaining of diarrhea of any age and sex groups from different locations (cities and villages)

You may qualify if:

• patients complaining of chronic diarrhea .

You may not qualify if:

• patients taking antibiotics within the previous four weeks, and anti-parasitic drugs within the previous two weeks.

Sponsors & Collaborators

• Sohag University: lead

Central Study Contacts

Khoulood Zakaria Hashem Abd El-Hafez, Assistant Lecturer

CONTACT

01127936972; Khoulood.zakaria@med.sohag.edu.eg

Shimaa Refaey Mohamed Abd-Elal, Assistant Lecturer

CONTACT

01144981097; Shimaa.refaee@med.sohag.edu.eg

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer of medical parasitology at the Faculty of Medicine

Study Record Dates

• First Submitted: February 2, 2023
• First Posted: February 13, 2023
• Study Start: March 1, 2023
• Primary Completion: March 1, 2024
• Study Completion: March 1, 2025
• Last Updated: February 13, 2023

Record last verified: 2023-02