Role of Mitophagy in Myeloid Cells During Coronary Atherosclerosis.

NCT05708547 | Completed

• 1 other identifier: NUGUYEN AOI 2022
• Study Type: observational
• Target: 61
• Locations: 1 country
• Sites: 1

Brief Summary

Atherosclerosis (deposition of a plaque essentially composed of lipids on the artery walls) is a frequent condition and is a leading cause of death worldwide. In addition to the long-established risk factors such as age, hypertension, diabetes or sedentary lifestyle, it has been demonstrated that immune cells can participate in the genesis of atherosclerotic plaques through metabolic and mitochondrial reprogramming. A non-invasive marker of this immune reprogramming has yet to be identified. Through the comparison of a group of atheromatous patients and a group of non-atheromatous patients, this study aims to evaluate this reprogramming phenomenon using a novel non-invasive method. This monocentric interventional study will take place at the Dijon Bourgogne University Hospital and will include 50 patients divided into 2 groups: "atheromatous coronary patients" and "non-atheromatous patients". The duration of participation in this study is 1 month. This study is based on usually performed procedures. Only blood samples will be taken on a catheter usually used during any cardiac surgery in addition to the medical care that is provided during hospitalization.

Conditions

Coronary Atherosclerosis

Outcome Measures

Primary Outcomes (1)

• Mitophagy level by flow cytometry

  Average fluorescence corresponding to PINK1-AF488 intracellular labelling (mitophagy checkpoint) in conventional (CD33+, CD66b-, CD14++, CD16-), intermediate (CD33+, CD66-, CD14++, CD16+), or non-conventional (CD33+, CD66b-, CD14+, CD16++) monocytes.

  Before the introduction of extracorporeal circulation.

Study Arms (2)

Atheromatous coronary patients

patients scheduled for coronary artery bypass surgery with extracorporeal circulation

Biological: Blood samples; Procedure: myocardial tissue samples; Other: Data collection

Non-atheratomous patients

Patients scheduled for valve or ascending aorta surgery with extracorporeal circulation without coronary lesion or peripheral arterial disease

Biological: Blood samples; Procedure: myocardial tissue samples; Other: Data collection

Interventions

Blood samples BIOLOGICAL

at the beginning of the extracorporeal circulation, at the end of the extracorporeal circulation and at D1

Atheromatous coronary patients; Non-atheratomous patients

myocardial tissue samples PROCEDURE

use of usually harvested right auricular tissue

Atheromatous coronary patients; Non-atheratomous patients

Data collection OTHER

pre-operative data: demographic data, severity scores, co-morbidities, treatments administered, collection of the presence and stage of arteriosclerotic disease, SYNTAX score Collection of data on the procedure Data from the clinical evaluation and daily biological examinations until D7 data from the follow-up consultation between D30 and D60: late complications, total length of stay in intensive care and hospital

Atheromatous coronary patients; Non-atheratomous patients

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Atheromatous coronary patients and non-coronary patients

You may qualify if:

• \- Person who provides oral consent
• Group 1:
• \- Patient scheduled for cardiac bypass surgery (isolated procedure) with extracorporeal circulation
• Group 2:
• Patient scheduled for valve ou ascending aorta surgery with extracorporeal circulation
• No coronary lesion
• No peripheral arterial disease (limbs, carotids, aortic aneurysm)

You may not qualify if:

• Person not affiliated with national health care system
• Medication that alters mitochondrial function (Chloroquine, hydroxychloroquine, rapamycin, carbamazepine, resveratrol, sildenafil)
• Person under a legal protection measure (curatorship, guardianship, tutorship)
• Pregnant, parturient or breastfeeding women
• Major unable to express consent
• Minor

Sponsors & Collaborators

• Centre Hospitalier Universitaire Dijon: lead

Study Sites (1)

Chu Dijon Bourgogne

Dijon, 21000, France

Location

MeSH Terms

Conditions

Coronary Artery Disease

Interventions

Blood Specimen Collection; Data Collection

Condition Hierarchy (Ancestors)

Coronary Disease; Myocardial Ischemia; Heart Diseases; Cardiovascular Diseases; Arteriosclerosis; Arterial Occlusive Diseases; Vascular Diseases

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques; Epidemiologic Methods; Health Care Evaluation Mechanisms; Quality of Health Care; Health Care Quality, Access, and Evaluation; Public Health; Environment and Public Health

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: January 23, 2023
• First Posted: February 1, 2023
• Study Start: November 14, 2023
• Primary Completion: October 16, 2025
• Study Completion: October 16, 2025
• Last Updated: November 21, 2025

Record last verified: 2025-11

Locations

FR (1)