NCT05677724|Unknown
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Sponsor study identifier
observational
20
1 country
1
Study Start
July 1, 2022
Actual (reported)Primary Completion
June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
In summary, with the help of single-cell sequencing technology, this study aims to focus on elucidating the influence of HBV-induced hepatocellular carcinoma cell metabolic changes on microenvironment remodeling. With the help of hepatocellular carcinoma microenvironment changes, this study provide a more accurate diagnosis and treatment method for HBV-induced hepatocellular carcinoma.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
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Some registry-reported names shown below are not yet linked in this graph.
Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
July 1, 2022
Actual (reported)October 18, 2022
Actual (reported)January 10, 2023
Actual (reported)June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Biochemical index detection
HbsAg (IU/mL) \\HbsAb (mIU/mL)\\HbeAg\\HbeAb\\HbcAb
within 4 hours after the sample was submitted for examination
HBV DNA copy number
HBV DNA (IU/mL)
within 24 hours after the sample was submitted for examination
single cell RNA sequencing
10x genomics chromium 3' sequencing
within 4 hours after the sample was submitted for examination
HBV DNA(\>20000IU/mL)
HBV DNA(\>2000IU/mL)
HBV DNA(10-2000IU/mL)
HBV DNA(=\<10IU/mL)
HBV DNA(0 IU/mL)
Clinical surgical specimens were obtained, cleaned briefly and transported to the laboratory. Single-cell suspension preparation and blood cell separation were performed, single-cell sorting suspension was prepared and put on the machine, sorting and library construction were performed, and mRNA sequencing adaptor was docked. Single cell data analysis. Based on the findings of single cell analysis, functional verification tests of tumor immune microenvironment cell groups and functional changes of tumor cells were designed.
Registry-reported names are shown in full. Names without a link have not yet been matched to a Syfrah profile.
A total of 20 patients with primary liver cancer, including 40 single cell sequencing samples (20 cases of liver cancer tissue and 20 cases of paired peripheral blood), were planned to be included and divided into 5 groups. Groups 1-4 were primary liver cancer complicated with hepatitis B infection, and group 5 were hepatitis B negative liver cancer patients.
Zhongnan Hospital of Wuhan University
Wuhan, Hubei, 430071, China
Fubing Wang, Doctor
Wuhan University
First Submitted
October 18, 2022
First Posted
January 10, 2023
Study Start
July 1, 2022
Actual (reported)Primary Completion
June 30, 2023
Estimated (date passed)Exploring the Application of 3D Bioprinting for Personalized Treatment in Primary Liver Cancer With Tumor Microenvironment
Contact the study team to confirm eligibility.
This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
June 30, 2023
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Last Updated
January 10, 2023
Record last verified: 2022-12
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Source: ClinicalTrials.gov.
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