Association Between Matrix Metalloproteinase-2 Gene Polymorphism rs243865 and Susceptibility to Cataract Development

NCT05670834 | Completed

• 1 other identifier: Soh-Med-22-12-06
• Study Type: observational
• Target: 90
• Locations: 1 country
• Sites: 1

Brief Summary

The primary aim of this study is to investigate the potential association between MMP-2 gene polymorphism and susceptibility to cataract development in Egyptian patients. Furthermore, this study aims to compare between cataract patients and healthy controls by measuring the serum IL-6 concentration to evaluate its relationship with disease.

Conditions

Cataract

Outcome Measures

Primary Outcomes (1)

• MMP-2 gene (rs243865) polymorphism in cataract patients and healthy controls

  Matrix Metalloproteinase-2 Gene Polymorphism using real-time PCR

  1 year maximum

Secondary Outcomes (1)

• compare between cataract patients and healthy controls by measuring the serum IL-6 concentration to evaluate its relationship with disease.

  1 year maximum

Study Arms (3)

control group (GroupA)

normal healthy non-cataractous volunteers aged ≥21 years

Genetic: genotyping assay by real-time PCR; Diagnostic Test: ELISA

Group B (experimental group 1)

cataractous patients aged \>50 years and diagnosed with senile cataract.

Genetic: genotyping assay by real-time PCR; Diagnostic Test: ELISA

Group C (experimental group 2)

cataractous patients aged ≥21 to 50 years and diagnosed with secondary cataract

Genetic: genotyping assay by real-time PCR; Diagnostic Test: ELISA

Interventions

genotyping assay by real-time PCR GENETIC

genotyping assay of MMP-2 gene with the real-time polymerase chain reaction

Group B (experimental group 1); Group C (experimental group 2); control group (GroupA)

ELISA DIAGNOSTIC_TEST

. IL-6 level in serum will be analyzed by ELISA assay kit.

Group B (experimental group 1); Group C (experimental group 2); control group (GroupA)

Eligibility Criteria

• Age: 21 Years+
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

cataractous patients in Department of ophthalmology in Sohag University Hospitals and healthy controls of matched age and sex

You may qualify if:

• Group A (control group) will include 30 eyes of 30 normal healthy non-cataractous volunteers aged ≥21 years. Group B (experimental group 1) will include 30 eyes of 30 cataractous patients aged \>50 years and diagnosed with senile cataract. Group C (experimental group 2) will include 30 eyes of 30 cataractous patients aged ≥21 to 50 years and diagnosed with secondary cataract. The control group will include participants who will be age-matched and sex-matched with the patients in the experimental groups

You may not qualify if:

• concomitant eye pathology or inflammation, systemic diseases and previous eye or systemic surgery.

Sponsors & Collaborators

• Sohag University: lead

Study Sites (1)

Faculty of Medicine Sohag University

Sohag, 82519, Egypt

Location

Related Publications (1)

• Dong Y, Mu GY, Chen F, Zhao RL, Wang M, Wang B. Correlation between MMP-2 gene polymorphism and cataract susceptibility. Eur Rev Med Pharmacol Sci. 2019 Apr;23(8):3167-3172. doi: 10.26355/eurrev_201904_17674.

  PMID: 31081067 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Blood sample will be obtained from all participants by withdrawing 3 mL of blood via venipuncture in EDTA tube

MeSH Terms

Conditions

Cataract

Condition Hierarchy (Ancestors)

Lens Diseases; Eye Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: demonstrator

Study Record Dates

• First Submitted: December 15, 2022
• First Posted: January 4, 2023
• Study Start: July 1, 2023
• Primary Completion: January 30, 2024
• Study Completion: April 30, 2024
• Last Updated: July 23, 2024

Record last verified: 2024-07

Locations

EG (1)