NCT05638581

Brief Summary

The goal of this clinical trial is to compare the effectiveness of unseparated whole blood (referred to as Low-Titer Group O Whole Blood) and the separate components of whole blood (including red cells, plasma, platelets, and cryoprecipitate) in critically injured patients who require large-volume blood transfusions.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,100

participants targeted

Target at P75+ for phase_3

Timeline
14mo left

Started Jul 2023

Typical duration for phase_3

Geographic Reach
1 country

13 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress71%
Jul 2023Jun 2027

First Submitted

Initial submission to the registry

November 8, 2022

Completed
28 days until next milestone

First Posted

Study publicly available on registry

December 6, 2022

Completed
8 months until next milestone

Study Start

First participant enrolled

July 27, 2023

Completed
3.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2027

Last Updated

August 21, 2025

Status Verified

August 1, 2025

Enrollment Period

3.4 years

First QC Date

November 8, 2022

Last Update Submit

August 15, 2025

Conditions

Keywords

Massive TransfusionTraumaShockHemorrhagePlasmaPlateletsRed Blood CellsLow-Titer Group O Whole BloodBlood components

Outcome Measures

Primary Outcomes (1)

  • 6-hour Mortality

    Participant vital status at 6-hours following randomization (randomization defined as product container is opened for administration to participant)

    First 6 hours after randomization

Secondary Outcomes (12)

  • 24-hour Mortality

    First 24 hours after randomization

  • Hospital/30-day Mortality

    From randomization to hospital discharge or 30-days post randomization (whichever the earlier)

  • Incidence of Pre-specified Complications

    From randomization to hospital discharge or 30-days post randomization (whichever the earlier)

  • Adjudicated Primary Cause of Death

    30-days post randomization

  • Length of Stay (Hospital and Intensive Care Unit)

    From randomization to hospital discharge or 30-days post randomization (whichever the earlier)

  • +7 more secondary outcomes

Study Arms (2)

LTOWB

ACTIVE COMPARATOR

Participants randomized to receive (Low Titer O Whole Blood \[LTOWB\])

Biological: LTOWB

Components

ACTIVE COMPARATOR

Participants randomized to receive the component blood products.

Biological: Components

Interventions

LTOWBBIOLOGICAL

Participants will receive Low Titer O Whole Blood administered intravenously or intraosseously.

LTOWB
ComponentsBIOLOGICAL

Participants will receive separated blood components (i.e., units of red cells, plasma, platelets, and cryoprecipitate) co-administered intravenously or intraosseously.

Components

Eligibility Criteria

Age15 Years+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Adult trauma patient (estimated age \> 15 or weight \> 50 kg, if age unknown)
  • Patient taken to trauma center directly from scene
  • Commencement of blood transfusion (PRBC, plasma or LTOWB), in pre-hospital or in-hospital setting
  • Activation of site-specific Massive Hemorrhage Protocol or Massive Transfusion Protocol
  • Traumatic injury with at least one of the following:
  • Confirmed or suspected acute major bleeding
  • Assessment of Blood Consumption (ABC) Score ≥2

You may not qualify if:

  • Patients who have received, prehospital or in-hospital more than two units of LTOWB; the equivalent in components (two units of packed red blood cells and two units of plasma); or a combination of the two (more than one unit of LTOWB, one unit of packed cells, and one unit of plasma). Most trauma centers hold two units of either packed red blood cells (with two units of plasma) or two units of LTOWB in the emergency department. This stock is used to initiate transfusion, while the massive hemorrhage protocol is activated from the blood bank.
  • Patients transferred from another hospital
  • Children \<15 years (in most communities, patients aged 15-18 years are treated at adult trauma centers, and patients in this age group frequently suffer life-threatening injuries, and will therefore be included)
  • Known prisoners, defined as individuals involuntarily confined or detained in a penal institution (including juvenile detention, involuntary psychiatric commitment, or court-ordered residential substance abuse treatment)
  • Moribund patients expected to die within 1 hour
  • Patients who required an ED thoracotomy or received more than 5 consecutive minutes of cardiopulmonary resuscitation (prior to receiving randomized blood products)
  • Patients with known "do not resuscitate" orders prior to randomization
  • Patients who refuse the administration of blood products
  • Individuals with a research "opt out" bracelet.
  • Greater than 20% total body surface area (TBSA) burns
  • Suspected inhalation injury victims
  • Patients who are obviously pregnant on clinical examination or known to be pregnant as provided by the subject or legally authorized representative

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (13)

University of Alabama at Birmingham, UAB Hospital

Birmingham, Alabama, 35294, United States

RECRUITING

Los Angeles County + University of Southern California (LAC + USC) Medical Center

Los Angeles, California, 90033, United States

NOT YET RECRUITING

University Medical Center New Orleans LCMC Health

New Orleans, Louisiana, 70112, United States

NOT YET RECRUITING

University of Maryland Medical Center

Baltimore, Maryland, 21201, United States

NOT YET RECRUITING

Washington University School of Medicine

St Louis, Missouri, 63110, United States

NOT YET RECRUITING

Atrium Health Wake Forest Baptist

Winston-Salem, North Carolina, 27157, United States

NOT YET RECRUITING

University of Cincinnati Medical Center

Cincinnati, Ohio, 45267, United States

RECRUITING

Oregon Health and Sciences University Hospital

Portland, Oregon, 97239, United States

NOT YET RECRUITING

Penn Presbyterian Medical Center

Philadelphia, Pennsylvania, 19104, United States

NOT YET RECRUITING

University of Texas Health Science Center Houston

Houston, Texas, 77030, United States

NOT YET RECRUITING

University of Texas Health San Antonio and University Health System

San Antonio, Texas, 78229, United States

NOT YET RECRUITING

Harborview Medical Center

Seattle, Washington, 98104, United States

RECRUITING

Froedtert Hospital

Milwaukee, Wisconsin, 53226, United States

NOT YET RECRUITING

Related Publications (2)

  • Jansen JO, Pedroza C, Novelo LL, Hao T, DeWildt GR, Coton CF, Mansoor K, Stephens SW, Marques MB, Stubbs JR, Richter JR, Wang HE, Holcomb JB, DeSantis SM. Trauma resuscitation with Low-Titer Group O Whole Blood Or Products: study protocol for a randomized clinical trial (the TROOP trial). Trials. 2025 Aug 2;26(1):266. doi: 10.1186/s13063-025-08971-y.

  • Meizoso JP, Cotton BA, Lawless RA, Kodadek LM, Lynde JM, Russell N, Gaspich J, Maung A, Anderson C, Reynolds JM, Haines KL, Kasotakis G, Freeman JJ. Whole blood resuscitation for injured patients requiring transfusion: A systematic review, meta-analysis, and practice management guideline from the Eastern Association for the Surgery of Trauma. J Trauma Acute Care Surg. 2024 Sep 1;97(3):460-470. doi: 10.1097/TA.0000000000004327. Epub 2024 Mar 27.

Related Links

MeSH Terms

Conditions

Wounds and InjuriesShock, HemorrhagicShockHemorrhage

Interventions

Elements

Condition Hierarchy (Ancestors)

Pathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Inorganic Chemicals

Study Officials

  • Jan Jansen, MBBS, PhD

    University of Alabama at Birmingham

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Shannon Stephens, EMTP, CCEMTP

CONTACT

Kiran Mansoor, MBBS

CONTACT

Study Design

Study Type
interventional
Phase
phase 3
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, OUTCOMES ASSESSOR
Masking Details
Care providers will be blinded to assignment until the point of randomization, which is when the cooler is opened, in the trauma bay, to remove blood products for transfusion.
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

November 8, 2022

First Posted

December 6, 2022

Study Start

July 27, 2023

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

June 30, 2027

Last Updated

August 21, 2025

Record last verified: 2025-08

Data Sharing

IPD Sharing
Will not share

Locations