Effect of Omega-3 Fatty Acids on Kynurenine Metabolism and Mood

NCT05520437 | Unknown

• 1 other identifier: 2019/35/N/NZ7/03757
• Study Type: interventional
• Target: 80
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of the current study is to explore the effect of omega-3 fatty acids supplementation on stress-induced changes to kynurenine metabolism and mood. The objectives of this research project are as follows:

1. 1.To compare biochemical parameters (KYN and KYNA, markers of oxidative stress, and markers of inflammation) between physically active and inactive males
2. 2.To determine the impact of a 3-month supplementation with omega-3 fatty acids on mood, inflammation markers and oxidative stress, and changes in KYN and KYNA
3. 3.To determine the impact of the stress manipulation test (TSST) on mood and changes in KYN and KYNA before and after the omega-3 fatty acids supplementation

Conditions

Stress-induced Mood; Kynurenine Pathway and Its Metabolites

Keywords

Omega-3; Kynurenine pathway

Outcome Measures

Primary Outcomes (1)

• Kynurenine pathway and its metabolites

  Before the intervention and 12 weeks after the start of the intervention blood samples will be collected from the participants to determine kynurenine pathway and its metabolites, namely Xanthurenic acid \[ng/mL\], Kynurenic acid \[ng/mL\], Kynurenine \[ng/mL\] and 3-Hydroxykynurenine \[ng/mL\]. This will be followed by inducing a stress response with the Trier Social Stress Test (TSST) in both groups of participants. Straight after TSST and one hour after the stress manipulation test Xanthurenic acid \[ng/mL\], Kynurenic acid \[ng/mL\], Kynurenine \[ng/mL\] and 3-Hydroxykynurenine \[ng/mL\] will be reassessed.

  3 months

Secondary Outcomes (5)

• Mood

  3 months

• Mood

  3 months

• Omega-3 fatty acids

  3 months

• Inflammation markers

  3 months

• Oxidative stress

  3 months

Study Arms (2)

Physically active males

EXPERIMENTAL

Half of the study population will consist of long-distance runners with 2-5 years of experience in running and similar times in covering the half-marathon distance.

Dietary Supplement: Omega-3 fatty acids; Dietary Supplement: Placebo

Physically inactive males

EXPERIMENTAL

The other half of the study population will consist of age-matched individuals involved in no regular physical activity.

Dietary Supplement: Omega-3 fatty acids; Dietary Supplement: Placebo

Interventions

Omega-3 fatty acids DIETARY_SUPPLEMENT

Participants received Omega-3 supplements (NAMEDSPORT) in the form of softgels over a period of 12 weeks. The supplement is certified by the International Fish Oil Standards™ (IFOS™) Program. Daily dose contained a total of 3276 mg of Omega 3 (4000 mg of fish oil of which: EPA 2234mg; DHA 916mg).

Physically active males; Physically inactive males

Placebo DIETARY_SUPPLEMENT

Participants will receive a placebo in the form of MCT oil capsules with no effect on the hypothesized outcome.

Physically active males; Physically inactive males

Eligibility Criteria

• Age: 28 Years - 48 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• long-distance runners with 2-5 years of experience in running and similar times in covering the half-marathon distance
• age-matched individuals involved in no regular physical activity
• must provide a signed informed consent form

You may not qualify if:

• DSM-5 psychiatric diagnoses other than depression and anxiety disorders (American Psychiatric Association, 2013)
• neurological disorders any other serious medical problems
• taking dietary supplements

Sponsors & Collaborators

• Medical University of Gdansk: lead
• National Science Centre, Poland: collaborator

Study Sites (1)

Medical University of Gdańsk

Gdansk, 80-210, Poland

Location

Related Publications (2)

• Bidzan-Wiacek M, Tomczyk M, Blazek M, Mika A, Antosiewicz J. No Effects of Omega-3 Supplementation on Kynurenine Pathway, Inflammation, Depressive Symptoms, and Stress Response in Males: A Placebo-Controlled Trial. Nutrients. 2024 Oct 31;16(21):3744. doi: 10.3390/nu16213744.

  PMID: 39519577 DERIVED

• Tomczyk M, Bidzan-Wiacek M, Kortas JA, Kochanowicz M, Jost Z, Fisk HL, Calder PC, Antosiewicz J. Omega-3 fatty acid supplementation affects tryptophan metabolism during a 12-week endurance training in amateur runners: a randomized controlled trial. Sci Rep. 2024 Feb 19;14(1):4102. doi: 10.1038/s41598-024-54112-x.

  PMID: 38374149 DERIVED

MeSH Terms

Interventions

Fatty Acids, Omega-3

Intervention Hierarchy (Ancestors)

Dietary Fats, Unsaturated; Dietary Fats; Fats; Lipids; Fatty Acids, Unsaturated; Fatty Acids; Fish Oils; Oils

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 14, 2021
• First Posted: August 30, 2022
• Study Start: November 20, 2020
• Primary Completion: December 1, 2022
• Study Completion: February 1, 2023
• Last Updated: August 30, 2022

Record last verified: 2022-08

Locations

PL (1)