NCT05517512

Brief Summary

the attendants of the clinic with a one-missed period (T0) underwent determination of baseline blood pressure (BP) measures and gave blood samples for estimation of levels of placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1) and pregnancy protein 13 (PP13). The same evaluation was repeated on the 6th, 24th, 32nd, and 36th gestational week (GW). Twenty non-pregnant women gave samples as a negative control group.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
138

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Mar 2020

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 12, 2020

Completed
1 year until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 14, 2021

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

March 19, 2022

Completed
5 months until next milestone

First Submitted

Initial submission to the registry

August 9, 2022

Completed
17 days until next milestone

First Posted

Study publicly available on registry

August 26, 2022

Completed
Last Updated

August 26, 2022

Status Verified

August 1, 2022

Enrollment Period

1 year

First QC Date

August 9, 2022

Last Update Submit

August 24, 2022

Conditions

Outcome Measures

Primary Outcomes (1)

  • Serum biomarkers and GHD

    The primary outcome is the ability of early ELISA estimation of biomarkers for prediction of GHD

    9 months

Study Arms (3)

Gestational Hypertension

Diagnostic Test: Estimation of Protein 13

Pre Eclampsia

Diagnostic Test: Estimation of Protein 13

Control

Diagnostic Test: Estimation of Protein 13

Interventions

Serum biomarkers' levels were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions and were read using a 96 well microplate ELISA reader (Dynatech. MR 7000) 1. Human placental growth factor (PLGF) was measured with the enzyme-linked immunoassay (ELISA) kit (catalog no. DPG00 SPG00 PDPG00, R\&D Systems Inc., Minneapolis, USA) by quantitative sandwich enzyme immunoassay technique (20). 2. Human soluble fms-like tyrosine kinase-1 (sFlt-1) was measured with the enzyme-linked immunoassay (ELISA) kit (catalog no. MBS601616, MyBioSource, Inc., California, San Diego, USA) by quantitative sandwich enzyme immunoassay technique (21). 3. Human pregnancy protein 13 was measured with the enzyme-linked immunoassay (ELISA) kit (catalog no. ab100553, Abcam Inc., Cambridge, USA) by quantitative sandwich enzyme immunoassay technique (22).

ControlGestational HypertensionPre Eclampsia

Eligibility Criteria

Age21 Years - 33 Years
Sexfemale
Age GroupsAdult (18-64)
Sampling MethodProbability Sample
Study Population

Non Hypertensive pregnant women

You may qualify if:

  • Normotensive women who attended the clinic with a one-missed period (T0)

You may not qualify if:

  • History of essential hypertension (HTN)
  • renal diseases
  • hepatic diseases
  • cardiac diseases
  • metabolic syndrome
  • body mass index (BMI) \>35 kg/ m2
  • congenital heart diseases

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Banha University

Banhā, El- Qalyobia, 13511, Egypt

Location

MeSH Terms

Conditions

Hypertension, Pregnancy-Induced

Condition Hierarchy (Ancestors)

Pregnancy ComplicationsFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesHypertensionVascular DiseasesCardiovascular Diseases

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant professor of gynecology

Study Record Dates

First Submitted

August 9, 2022

First Posted

August 26, 2022

Study Start

March 12, 2020

Primary Completion

March 14, 2021

Study Completion

March 19, 2022

Last Updated

August 26, 2022

Record last verified: 2022-08

Locations