Recombinant, Bivalent, Norovirus Vaccine Study

NCT05508178 | Completed Phase 1 DMC

• 1 other identifier: rNV-2v-001
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

This clinical study will evaluate the vaccine candidate rNV-2v, which is under development to prevent disease triggered by noroviruses. Noroviruses are one of the leading causes of gastrointestinal diseases in the world. Norovirus infections can cause vomiting, diarrhea, and cramping. Noroviruses can spread easily, especially in hospitals, schools, military barracks and ships. At the moment, there is no vaccine available to prevent norovirus infections or disease. This clinical trial will look at the safety and tolerability of an investigational vaccine that is being developed to prevent norovirus-related disease. The trial will also look at whether the immune system produces a response to the investigational study vaccine. The study vaccine is a combination of two different types of norovirus antigens. In contrast to similar vaccines under development, the vaccine studied here adds no substances (adjuvants) to increase or modulate the immune response. The study vaccine is produced using a plant-based system rather than a typically used animal cell system. This is the first time the study vaccine will be given to humans. Two different doses of the investigational study vaccine will be tested in this trial. Either the investigational study vaccine or the placebo will be given as 2 injections. These injections will be given about 1 month apart. The trial will last about 12 months, from the time of enrollment.

Conditions

Norovirus

Keywords

Norovirus; Vaccine; Adults; Safety; Immunogenicity; First-in-human

Outcome Measures

Primary Outcomes (3)

• To assess the safety, tolerability, and reactogenicity of rNV-2v administered intramuscularly (IM) to healthy subjects

  Safety and tolerability as determined by: * The occurrence of adverse events (AEs) consisting of local and systemic signs and symptoms within 7 days after vaccination. Local and systemic signs and symptoms, assessed as typical and expected reactions to study vaccine administration including pain, redness, and swelling at the injection site; fever, fatigue, gastrointestinal symptoms (nausea, vomiting, diarrhea, and/or abdominal pain), headache, myalgia, shivering, and arthralgia. * Incidence of abnormal hematology and serum chemistry laboratory values. * Occurrence of serious adverse events (SAEs). * Onset of any significant new medical conditions following the first administration of study vaccine.

  within 7 days after each administration of the vaccine

• To assess the safety, tolerability, and reactogenicity of rNV-2v administered intramuscularly (IM) to healthy subjects

  Safety and tolerability as determined by: * Incidence of abnormal hematology and serum chemistry laboratory values. * Occurrence of serious adverse events (SAEs). * Onset of any significant new medical conditions following the first administration of study vaccine.

  until 28 days after second administration of study vaccine (Day 57)

• To assess the safety, tolerability, and reactogenicity of rNV-2v administered intramuscularly (IM) to healthy subjects

  Safety and tolerability as determined by: * Occurrence of serious adverse events (SAEs). * Onset of any significant new medical conditions following the first administration of study vaccine.

  for 364 days following the first administration of study vaccine

Secondary Outcomes (1)

• To assess the humoral immune response elicited by rNV-2v by measuring binding antibodies against the GI.4 and GII.4 components of the bivalent vaccine

  Pre-dose, Week 1, Week 4, Week 8, Week 26, and Week 52.

Study Arms (3)

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (100 µg)

EXPERIMENTAL

Participants 18-40 years of age, 2-dose regimen: Norovirus bivalent GI.4 (50 μg) / GII.4 (50 μg) virus-like particle (VLP) vaccine, intramuscularly (IM), on Day 1 and Day 29

Biological: Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (100 µg)

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (300 µg)

EXPERIMENTAL

Participants 18-40 years of age, 2-dose regimen: Norovirus bivalent GI.4 (150 μg) / GII.4 (150 μg) virus-like particle (VLP) vaccine, intramuscularly (IM), on Day 1 and Day 29

Biological: Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (300 µg)

Placebo

PLACEBO COMPARATOR

Participants 18-40 years of age, 2-dose regimen: Placebo (norovirus vaccine vehicle without antigens), intramuscularly (IM), on Day 1 and Day 29

Drug: Placebo

Interventions

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (100 µg) BIOLOGICAL

Norovirus: 50 µg GI.4 VLP + 50 µg GII.4 VLP without adjuvant

Also known as: rNV-2v

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (100 µg)

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (300 µg) BIOLOGICAL

Norovirus: 150 µg GI.4 VLP + 150 µg GII.4 VLP without adjuvant

Also known as: rNV-2v

Norovirus GI.4 / GII.4 Bivalent VLP Vaccine (300 µg)

Placebo DRUG

Norovirus vaccine (rNV-2v) vehicle without antigen

Also known as: Vehicle

Placebo

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Capable of giving signed informed consent, which includes compliance with the requirements and restrictions listed in the informed consent form (ICF) and in this protocol.
• Male or female aged 18 to 40 years (inclusive) at Screening.
• Good general health as determined by a Screening evaluation that includes vital signs, medical history, physical examinations, and laboratory assessments within 28 days before administration of the study vaccine.
• Expressed interest, availability and understanding to fulfil the study requirements, and in the opinion of the Investigator, can and will comply with the protocol requirements.
• Female subjects of childbearing potential must be using a highly-effective licensed method of birth control for 30 days prior to the first vaccination and must agree to continue such precautions during the study until 60 days after the second vaccination. Male subjects must agree to employ barrier contraception from the day of first vaccination until 60 days after the second vaccination, if their female partner of childbearing potential doesn't use a highly effective method of birth control.
• Agrees not to participate in another clinical study with an investigational product or to use any nonregistered product (drug, vaccine, or medical device) for the duration of the study.

You may not qualify if:

• Any condition that may interfere with study participation or place the participant at increased risk of AEs would be excluded from the study. Additionally, subjects are excluded from the study if any of the following criteria apply:
• Positive serology test results for hepatitis C virus, human immunodeficiency virus antibody, and/or hepatitis B surface antigen at Screening.
• Pregnant or lactating women.
• History of any of the following medical illnesses, which, in the opinion of the Investigator, may interfere with study participation or place the participant at increased risk of AEs, including but not limited to: immunosuppression (disease or treatments that may affect immune system function), diabetes, cancer (malignancy other than a resolved or excised skin lesion), heart disease (hospitalisation for a heart attack, arrhythmia, or syncope), unconsciousness (excluding single and brief concussion), seizures, asthma requiring treatment with inhaler or medication in the past 2 years, neuroinflammatory disease, autoimmune disease, recurrent infections (more than 3 hospitalisations for invasive bacterial infections such as pneumonia or meningitis).
• Any current or chronic conditions requiring daily medication other than vitamins, minerals, or antihypertensives, as per the Investigator's discretion. Rescreening for acute illness that is expected to resolve quickly is allowed, as per the Investigator's discretion.
• Known or suspected allergies or hypersensitivity to any component of the study vaccine.
• Any clinically significant abnormality detected during physical examinations or vital sign evaluations, as per the Investigator's discretion.
• Hypertension defined as a mean of triplicate sitting blood pressure measurements \>150/90 mmHg.
• Any Screening hematology or biochemistry abnormalities considered clinically significant by the Investigator.
• For women of child bearing potential, positive urine/serum pregnancy test at Screening or within 24 hours of the first dose of the study vaccine.
• Body (oral) temperature \>38°C or symptoms of an acute self-limited illness such as an upper respiratory infection or gastroenteritis within 3 days of administration of the first dose of the study vaccine.
• Administration of rNV-2v or any other experimental norovirus vaccine in the past.
• Planned administration of other vaccines from 14 days before the first administration of the study vaccine and 60 days after the last study administration of the study vaccine, with the exception of inactivated influenza vaccine, which can be administered up to 14 days before or from 30 days after the last administration of the study vaccine.
• Administration of immunoglobins or any blood products within 180 days prior to the first administration of the study vaccine and throughout the study duration, as per the Investigator's discretion.
• Relatives of the Sponsor, clinical research organisation, or the study centre personnel are excluded from participating in the study.

Sponsors & Collaborators

• Icon Genetics GmbH: lead
• University Hospital, Ghent: collaborator

Study Sites (1)

Center for Vaccinology (CEVAC), Ghent University and University Hospital

Ghent, 9000, Belgium

Location

Related Publications (2)

• Waerlop G, Janssens Y, Jacobs B, Jarczowski F, Diessner A, Leroux-Roels G, Klimyuk V, Leroux-Roels I, Thieme F. Immune responses in healthy adults elicited by a bivalent norovirus vaccine candidate composed of GI.4 and GII.4 VLPs without adjuvant. Front Immunol. 2023 Jun 26;14:1188431. doi: 10.3389/fimmu.2023.1188431. eCollection 2023.

  PMID: 37435073 DERIVED

• Leroux-Roels I, Maes C, Joye J, Jacobs B, Jarczowski F, Diessner A, Janssens Y, Waerlop G, Tamminen K, Heinimaki S, Blazevic V, Leroux-Roels G, Klimyuk V, Adachi H, Hiruta K, Thieme F. A randomized, double-blind, placebo-controlled, dose-escalating phase I trial to evaluate safety and immunogenicity of a plant-produced, bivalent, recombinant norovirus-like particle vaccine. Front Immunol. 2022 Oct 7;13:1021500. doi: 10.3389/fimmu.2022.1021500. eCollection 2022.

  PMID: 36275772 DERIVED

Related Links

• Center for Disease Control and Prevention norovirus information webpage

Study Officials

• Principal Investigator

  Center for Vaccinology (CEVAC), Ghent University and University Hospital, Ghent, Belgium.

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: PREVENTION
• Intervention Model: SEQUENTIAL
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 17, 2022
• First Posted: August 19, 2022
• Study Start: August 17, 2020
• Primary Completion: October 26, 2021
• Study Completion: October 26, 2021
• Last Updated: August 19, 2022

Record last verified: 2022-08

Data Sharing

• IPD Sharing: Will not share

Locations

BE (1)