Gasdermin-D and Pannexin-1 in Pregnancy

NCT05475951 | Completed

• 1 other identifier: 95932
• Study Type: interventional
• Target: 79
• Locations: 1 country
• Sites: 1

Brief Summary

One -third of fertile women around the world are overweight or obese. This means increasing risk for both the mother and the child. Fat tissue is a factor in gestational DM development and the increase in material inflammation and oxidative stress. According to new knowledge, it is an important need to examine molecules that are not handled in new and human research in these mechanisms in fat and placenta tissues in obesity. For this purpose, the examination of the expression of gasdermin-D and pannex-1 proteins, which are shown to be produced in the cells of both tissues, is worth investigating in human fat tissue and placenta. Gasdermins and pannexins are proteins intersecting by interacting in cellular functions. Gasdermins cause piroptosis, a type of litic proinflammatory cell death. Pannexin-1 plays in various cellular functions, including inflammation. These are examined in a small number of in vitro studies in material fat tissue and placenta, and the design of this study in people whose applications are applied is original in humans. The status of the expressions of the gasdermin-D and pannexin-1 proteins, which will be examined for the first time in obese pregnant women's fat and placental tissues, are the subject of this research with each other and their relationship with other maternal and neonatal data.

Conditions

Pregnancy Complications; Maternal Obesity

Keywords

gasdermin-D; pannexin-1; placenta; omental tissue; fat tissue; maternal obesity; inflammation; oxidative stress; biochemistry; ELISA

Outcome Measures

Primary Outcomes (1)

• Prediction of macrosomy

  Prediction of macrosomy will be assesed by meaningfull increases of studied biomarkers Gasdermin-D and Pannexin-1

  7 months

Study Arms (3)

Normal Weight Pregnant Women

EXPERIMENTAL

The women who had term pregnancy and had normal BMI.

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Overweight Pregnant Women

EXPERIMENTAL

The women who had term pregnancy and had BMI values between 25-30.

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Obese Pregnant Women

EXPERIMENTAL

The women who had term pregnancy, and had BMI values more than 30.

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Interventions

Biomarker measurement of pannexin-1 and gasdermin-D DIAGNOSTIC_TEST

These biomarkers were measured in the placental and adipose tissues of the participants.

Normal Weight Pregnant Women; Obese Pregnant Women; Overweight Pregnant Women

Eligibility Criteria

• Age: 20 Years - 35 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Being in the 20-35 age range
• Term pregnancy (37-41 weeks)
• Healthy pregnancy follow-up
• Planned cesarean section
• Having no complication in cesarean delivery
• Being a placenta with front or rear walls

You may not qualify if:

• Intrauterine surgical procedure
• Presence of prenatal placental structural disorder
• Multiple pregnancies

Sponsors & Collaborators

• Haseki Training and Research Hospital: lead

Study Sites (1)

Haseki Training and Research Hospital

Sultangazi, Istanbul, 34000, Turkey (Türkiye)

Location

Related Publications (11)

• Estienne A, Bongrani A, Reverchon M, Rame C, Ducluzeau PH, Froment P, Dupont J. Involvement of Novel Adipokines, Chemerin, Visfatin, Resistin and Apelin in Reproductive Functions in Normal and Pathological Conditions in Humans and Animal Models. Int J Mol Sci. 2019 Sep 9;20(18):4431. doi: 10.3390/ijms20184431.

  PMID: 31505789 BACKGROUND

• Rancourt RC, Ott R, Ziska T, Schellong K, Melchior K, Henrich W, Plagemann A. Visceral Adipose Tissue Inflammatory Factors (TNF-Alpha, SOCS3) in Gestational Diabetes (GDM): Epigenetics as a Clue in GDM Pathophysiology. Int J Mol Sci. 2020 Jan 12;21(2):479. doi: 10.3390/ijms21020479.

  PMID: 31940889 BACKGROUND

• Broz P, Pelegrin P, Shao F. The gasdermins, a protein family executing cell death and inflammation. Nat Rev Immunol. 2020 Mar;20(3):143-157. doi: 10.1038/s41577-019-0228-2. Epub 2019 Nov 5.

  PMID: 31690840 BACKGROUND

• Abrahams VM, Tang Z, Mor G, Guller S. NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells. J Reprod Immunol. 2020 Nov;142:103214. doi: 10.1016/j.jri.2020.103214. Epub 2020 Oct 3.

  PMID: 33152658 BACKGROUND

• Zhang C, Zhao C, Chen X, Tao R, Wang S, Meng G, Liu X, Shao C, Su X. Induction of ASC pyroptosis requires gasdermin D or caspase-1/11-dependent mediators and IFNbeta from pyroptotic macrophages. Cell Death Dis. 2020 Jun 18;11(6):470. doi: 10.1038/s41419-020-2664-0.

  PMID: 32555186 BACKGROUND

• Dye ZT, Rutledge LV, Penuela S, Dyce PW. Pannexin 1 inhibition delays maturation and improves development of Bos taurus oocytes. J Ovarian Res. 2020 Aug 24;13(1):98. doi: 10.1186/s13048-020-00704-w.

  PMID: 32838805 BACKGROUND

• Chen KW, Demarco B, Heilig R, Shkarina K, Boettcher A, Farady CJ, Pelczar P, Broz P. Extrinsic and intrinsic apoptosis activate pannexin-1 to drive NLRP3 inflammasome assembly. EMBO J. 2019 May 15;38(10):e101638. doi: 10.15252/embj.2019101638. Epub 2019 Mar 22.

  PMID: 30902848 BACKGROUND

• Korac A, Srdic-Galic B, Kalezic A, Stancic A, Otasevic V, Korac B, Jankovic A. Adipokine signatures of subcutaneous and visceral abdominal fat in normal-weight and obese women with different metabolic profiles. Arch Med Sci. 2021 Feb 26;17(2):323-336. doi: 10.5114/aoms/92118. eCollection 2021.

  PMID: 33747267 BACKGROUND

• Stubert J, Reister F, Hartmann S, Janni W. The Risks Associated With Obesity in Pregnancy. Dtsch Arztebl Int. 2018 Apr 20;115(16):276-283. doi: 10.3238/arztebl.2018.0276.

  PMID: 29739495 BACKGROUND

• Poston L, Harthoorn LF, Van Der Beek EM; Contributors to the ILSI Europe Workshop. Obesity in pregnancy: implications for the mother and lifelong health of the child. A consensus statement. Pediatr Res. 2011 Feb;69(2):175-80. doi: 10.1203/PDR.0b013e3182055ede.

  PMID: 21076366 BACKGROUND

• Tozzi M, Hansen JB, Novak I. Pannexin-1 mediated ATP release in adipocytes is sensitive to glucose and insulin and modulates lipolysis and macrophage migration. Acta Physiol (Oxf). 2020 Feb;228(2):e13360. doi: 10.1111/apha.13360. Epub 2019 Oct 14.

  PMID: 31400255 RESULT

MeSH Terms

Conditions

Pregnancy Complications; Pregnancy in Obesity; Inflammation

Condition Hierarchy (Ancestors)

Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Obesity; Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Pathologic Processes

Study Officials

• Ali Cetin, Professor

  Departmant of Obstetrics and Gynecology in Hasek, Training and Research Hospital, Istanbul, Turkey

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 6, 2022
• First Posted: July 27, 2022
• Study Start: December 1, 2021
• Primary Completion: May 15, 2022
• Study Completion: May 30, 2022
• Last Updated: July 27, 2022

Record last verified: 2022-05

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)