NCT05290428

Brief Summary

Based on the previous study of NCT03961191 and NCT03960879, we performed this trial to further confirm the accuracy of host DNA PAX1 and JAM3 methylation for cervical cancer screening. This study would provide profound basis for the approval of assay kit of DNA methylation in China for cervical screening. Three hospitals, including Peking Union Medical College Hospital, would enroll eligible patients in this study. The cervical cytology of 3 ml will be collected for the detection of DNA PAX1 and JAM3 methylation, and the results will compared with the cervical histological pathology, which is achieved after collection of cervical cytology, by surgeries including loop electrosurgical excision procedure, cervical conization, total hysterectomy and others. The methylation testing would be double-blinded in operators and analysts. The retrospective and prospective parts will enroll at least 120 patients and at least 339 patients, respectively.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
459

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Mar 2022

Shorter than P25 for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 12, 2022

Completed
Same day until next milestone

Study Start

First participant enrolled

March 12, 2022

Completed
10 days until next milestone

First Posted

Study publicly available on registry

March 22, 2022

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 12, 2022

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 12, 2022

Completed
Last Updated

March 22, 2022

Status Verified

March 1, 2022

Enrollment Period

6 months

First QC Date

March 12, 2022

Last Update Submit

March 12, 2022

Conditions

Outcome Measures

Primary Outcomes (2)

  • Sensitivity of methylation testing

    Sensitivity of methylation testing of cervical cytology compared with histology

    One week

  • Specificity of methylation testing

    Specificity of methylation testing of cervical cytology compared with histology

    One week

Secondary Outcomes (2)

  • Positive predictive value of methylation testing

    One week

  • Negative predictive value of methylation testing

    One week

Study Arms (2)

Retrospective part

This part will enroll at least 120 cases in previously preserved samples.

Diagnostic Test: DNA methylation testing

Prospective part

This part will enroll at least 339 cases.

Diagnostic Test: DNA methylation testing

Interventions

All cervical cytology samples will be tested for PAX1 and JAM3 methylation

Prospective partRetrospective part

Eligibility Criteria

Age18 Years+
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

All participants will accept surgeries harvesting cervical histology.

You may qualify if:

  • With uterine cervix intact
  • Aged 18 years or older
  • With accessible histological results of cervix
  • Signed an approved informed consents
  • With accessible cervical cytology before harvesting cervical histology

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Lei Li

Beijing, Beijing Municipality, 100730, China

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Cervical cytology

MeSH Terms

Conditions

Uterine Cervical NeoplasmsUterine Cervical Dysplasia

Condition Hierarchy (Ancestors)

Uterine NeoplasmsGenital Neoplasms, FemaleUrogenital NeoplasmsNeoplasms by SiteNeoplasmsUterine Cervical DiseasesUterine DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesPrecancerous Conditions

Study Officials

  • Lei Li, M.D.

    Peking Union Medical College Hospital

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
OTHER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor

Study Record Dates

First Submitted

March 12, 2022

First Posted

March 22, 2022

Study Start

March 12, 2022

Primary Completion

September 12, 2022

Study Completion

December 12, 2022

Last Updated

March 22, 2022

Record last verified: 2022-03

Locations