Understanding the Burn Wound Microbiome: Comparing Traditional Wound Cultures to Next Generation Sequencing Technology

NCT05275335 | Recruiting

• 1 other identifier: 21-0012
• Study Type: observational
• Target: 400
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this investigation is to better understand the wound microbiome in burn wounds and the role it plays in outcomes and complications related to treatment.

Conditions

Microbial Colonization; Burns

Outcome Measures

Primary Outcomes (1)

• Statistical correlation of the Next-Generation Sequenced microbiome to standard culture results in burn wounds

  The relationship between next-generation sequencing of the burn wound microbiome and traditional clinical culture techniques will be examined. In particular, comparisons will compare the bacterial microbiota of each sample with bacteria identified using standard clinical culture methods from the same sample. Correlations in taxonomic diversity will be done with linear regression and Pearson correlation.

  3 years

Secondary Outcomes (1)

• Clinical predictors of microbiome taxonomy

  3 years

Eligibility Criteria

• Age: 18 Years - 100 Years
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Adult burn patients with excised burn eschar

You may qualify if:

• All adult patients (18 years of age and greater) admitted to or consulted by the Acute Burn Surgery service with a diagnosis of a partial thickness or full thickness burn wound determined by the Acute Burn Surgery service to be appropriate for operative intervention. We will include patients 18 years of age and older of any gender, race, prior therapy, or pre-existing medical condition. We will also include subjects in special classes, including pregnant women and cognitively impaired persons.

Sponsors & Collaborators

• The University of Texas Medical Branch, Galveston: lead

Study Sites (1)

University of Texas Medical Branch

Galveston, Texas, 77550, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Excised and discarded burn wound eschar with microbial DNA analysis

MeSH Terms

Conditions

Communicable Diseases; Burns

Condition Hierarchy (Ancestors)

Infections; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Wounds and Injuries

Study Officials

• Steven E Wolf, MD

  University of Texas

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Steven E Wolf, MD

CONTACT

12107870507; swolf@utmb.edu

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 1 Day
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 11, 2022
• First Posted: March 11, 2022
• Study Start: November 1, 2022
• Primary Completion (Estimated): December 31, 2026
• Study Completion (Estimated): December 31, 2026
• Last Updated: August 15, 2025

Record last verified: 2025-08

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)