NCT05261542

Brief Summary

This study was designed to investigate the efficacy of flow cytometry to accurately identify between normal and cancer cells in colon epithelium in humans diagnosed with colorectal cancer.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
106

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Jan 2017

Longer than P75 for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 26, 2017

Completed
4.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 30, 2021

Completed
2 months until next milestone

Study Completion

Last participant's last visit for all outcomes

November 25, 2021

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

February 6, 2022

Completed
24 days until next milestone

First Posted

Study publicly available on registry

March 2, 2022

Completed
Last Updated

March 2, 2022

Status Verified

February 1, 2022

Enrollment Period

4.7 years

First QC Date

February 6, 2022

Last Update Submit

February 18, 2022

Conditions

Outcome Measures

Primary Outcomes (1)

  • Accuracy of intraoperative Flow Cytometry for cancer cell identification in colorectal cancer.

    Samples from cancer and normal colon epithelium are prospectively collected intraoperatively and assessed with intraoperative Flow Cytometry. Following comparative DNA content and phenotypic analysis, the receiver operating characteristic (ROC) curve analysis is used to evaluate the performance of intraoperative Flow Cytometry to distinguish between normal and cancer tissue. The optimal cut-off value is determined to delineate the status of tumor tissue, resulting in the highest sensitivity and specificity and hence the optimal accuracy.

    2017-2021

Study Arms (2)

normal colorectal epithelium

Tissue samples from normal colorectal epithelium are collected during colectomy in every colorectal cancer patient

Diagnostic Test: flow cytometry

cancer colorectal epithelium

Tissue samples from colorectal cancer are collected during colectomy in every colorectal cancer patient

Diagnostic Test: flow cytometry

Interventions

flow cytometryDIAGNOSTIC_TEST

evaluation of intraoperative flow cytometry in detecting cancer cells in colorectal epithelium

cancer colorectal epitheliumnormal colorectal epithelium

Eligibility Criteria

Age25 Years - 90 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

patients diagnosed with colorectal cancer and being treated by standard colectomy or/and neoadjuvant theraphy according to current guidelines.

You may qualify if:

  • biopsy proven colorectal cancer signed informed consent for participation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University Hospital of Ioannina

Ioannina, Epirus, 45500, Greece

Location

Biospecimen

Retention: SAMPLES WITH DNA

tissue samples from normal and cancer epithelium collected following colectomy

MeSH Terms

Conditions

Colorectal Neoplasms

Interventions

Flow Cytometry

Condition Hierarchy (Ancestors)

Intestinal NeoplasmsGastrointestinal NeoplasmsDigestive System NeoplasmsNeoplasms by SiteNeoplasmsDigestive System DiseasesGastrointestinal DiseasesColonic DiseasesIntestinal DiseasesRectal Diseases

Intervention Hierarchy (Ancestors)

Cell SeparationCytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisCytophotometryFluorometryLuminescent MeasurementsPhotometryChemistry Techniques, AnalyticalInvestigative Techniques

Study Design

Study Type
observational
Observational Model
OTHER
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
ACCOSIATE PROFESOR OF SURGERY

Study Record Dates

First Submitted

February 6, 2022

First Posted

March 2, 2022

Study Start

January 26, 2017

Primary Completion

September 30, 2021

Study Completion

November 25, 2021

Last Updated

March 2, 2022

Record last verified: 2022-02

Locations