NCT05248230

Brief Summary

This is a Phase 1/2 multicenter, open-label, single dose trial of 4D-710 investigational gene therapy in adults with cystic fibrosis.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
30

participants targeted

Target at P25-P50 for phase_2

Timeline
62mo left

Started Mar 2022

Longer than P75 for phase_2

Geographic Reach
1 country

20 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress45%
Mar 2022Jun 2031

First Submitted

Initial submission to the registry

February 10, 2022

Completed
11 days until next milestone

First Posted

Study publicly available on registry

February 21, 2022

Completed
1 month until next milestone

Study Start

First participant enrolled

March 29, 2022

Completed
5.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2027

Expected
4 years until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2031

Last Updated

March 11, 2026

Status Verified

March 1, 2026

Enrollment Period

5.2 years

First QC Date

February 10, 2022

Last Update Submit

March 9, 2026

Conditions

Keywords

CFCystic FibrosisGene Therapy

Outcome Measures

Primary Outcomes (1)

  • Incidence and severity of adverse events

    Safety and tolerability of 4D-710 following a single dose via inhalation, as assessed by incidence and severity of treatment emergent adverse events, serious adverse events, and dose limiting toxicities, including clinically significant changes from baseline to scheduled time points in safety parameters.

    60 Months

Study Arms (3)

4D-710 Phase 1: Dose Exploration

EXPERIMENTAL

Participants who are ineligible for or intolerant of modulator therapy will receive one of various dose levels of 4D-710 to identify recommended phase 2 dose(s) for further evaluation.

Biological: 4D-710

4D-710 Phase 2: Dose Expansion

EXPERIMENTAL

Participants will receive a single inhalational administration of 4D-710 at the dose level(s) selected for dose expansion.

Biological: 4D-710

4D-710 Dose Exploration (Sub-Study)

EXPERIMENTAL

Participants who are on currently available CFTR modulator therapy will receive a dose of 4D-710 at various dose levels.

Biological: 4D-710

Interventions

4D-710BIOLOGICAL

4D-710 is an adeno-associated virus (AAV) gene therapy comprised of an AAV capsid variant (4D-A101) carrying a transgene cassette encoding human cystic fibrosis transmembrane conductance regulator with a deletion in the regulatory domain (CFTRΔR).

4D-710 Dose Exploration (Sub-Study)4D-710 Phase 1: Dose Exploration4D-710 Phase 2: Dose Expansion

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • years and older
  • Confirmed diagnosis of cystic fibrosis (CF) and CF lung disease including:
  • Sweat chloride ≥ 60 mmol/L
  • Mutation Status
  • Bi-allelic mutations in the CFTR gene, or
  • Single mutation in the CFTR gene and clinical manifestations of CF lung disease
  • Ineligible for CFTR modulator therapy, or previously received modulator therapy but discontinued due to adverse effects.
  • Forced expiratory volume in 1 second (FEV1) ≥ 50% and ≤ 90% of predicted (per Global Lung Function Initiative) at Screening
  • Resting oxygen saturation ≥ 92% on room air at Screening
  • years and older
  • Confirmed diagnosis of cystic fibrosis (CF) and CF lung disease including:
  • Sweat chloride ≥ 60 mmol/L
  • Mutation Status
  • Bi-allelic mutations in the CFTR gene, or
  • Single mutation in the CFTR gene and clinical manifestations of CF lung disease
  • +2 more criteria

You may not qualify if:

  • Active Mycobacterium abscessus infection requiring ongoing treatment at Screening
  • Active allergic bronchopulmonary aspergillosis requiring management with systemic corticosteroids or antifungal therapy
  • Smoking (e.g. cigarettes, cigars, cannabis) or use of a vaping product during the 2 months prior to screening; must agree to not smoke or use vaping products during Screening and initial 24-month observation period.
  • Contraindication to systemic corticosteroid therapy
  • Requires chronic use of systemic corticosteroids or immunosuppressants to treat another condition
  • If no known diagnosis of cystic fibrosis related diabetes (CFRD), Type I, or Type II diabetes: Hemoglobin A1C ≥ 6.5% at Screening
  • If known diagnosis of CFRD, Type I or Type II diabetes: Hemoglobin A1C \> 7.5% at Screening
  • Recent history of symptomatic hyperglycemia or unstable blood glucose levels as per Investigator's assessment
  • Other conditions that, in the Investigator's opinion, may interfere with management of corticosteroid-related hyperglycemia
  • Body Mass Index (BMI) \< 16
  • Laboratory abnormalities at screening:
  • ALT, AST or GGT ≥ 3 × the upper limit of normal (ULN)
  • Total bilirubin ≥ 2 × ULN
  • Hemoglobin \< 10 g/dL
  • Requirement for continuous or night-time oxygen supplementation
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (20)

University of Alabama Child Health Research Unit

Birmingham, Alabama, 35233, United States

RECRUITING

The University of Arizona

Tucson, Arizona, 85724, United States

RECRUITING

University of California San Francisco

San Francisco, California, 94143, United States

RECRUITING

National Jewish Health

Denver, Colorado, 80206, United States

RECRUITING

University of Florida

Gainesville, Florida, 32610, United States

RECRUITING

University of Miami Hospital

Miami, Florida, 33136, United States

RECRUITING

Northwestern Memorial Hospital

Chicago, Illinois, 60611, United States

RECRUITING

University of Kansas Medical Center

Kansas City, Kansas, 66160, United States

RECRUITING

Johns Hopkins Hospital

Baltimore, Maryland, 21287, United States

RECRUITING

Massachusetts General Hospital

Boston, Massachusetts, 02114, United States

RECRUITING

Boston Children's Hospital

Boston, Massachusetts, 02115, United States

RECRUITING

University of North Carolina at Chapel Hill

Chapel Hill, North Carolina, 27599, United States

COMPLETED

Rainbow Babies and Children's Hospital/University Hospitals Cleveland Medical Center

Cleveland, Ohio, 44146, United States

RECRUITING

Nationwide Children's Hospital

Columbus, Ohio, 43205, United States

RECRUITING

Penn State Health

Hershey, Pennsylvania, 17033, United States

RECRUITING

The Hospital of the University of Pennsylvania

Philadelphia, Pennsylvania, 19104, United States

RECRUITING

Medical University of South Carolina

Charleston, South Carolina, 29425, United States

RECRUITING

The University of Texas Southwestern Medical Center

Dallas, Texas, 75390, United States

RECRUITING

Virginia Commonwealth University Health System

Richmond, Virginia, 23298, United States

RECRUITING

University of Washington Medical Center

Seattle, Washington, 98195, United States

RECRUITING

Related Publications (1)

  • Calton MA, Croze RH, Sullivan TH, Collins SA, Tucker S, Whittlesey KJ, Kim DH, Nye JA, Beliakoff G, Quezada M, Burns C, Schmitt C, Klein A, Jia V, Kovacs L, Lauko D, Yoh K, Nguyen K, Barglow K, Gonzales J, Khoday D, Mason T, Delaria K, Bashour K, Kotterman M, Schaffer D, Song A, Francis P, Taylor-Cousar JL, Kirn D. Design and Characterization of 4D-710, an Aerosolized Gene Therapy for Cystic Fibrosis Lung Disease. Am J Respir Cell Mol Biol. 2025 Oct 22. doi: 10.1165/rcmb.2025-0243MA. Online ahead of print.

MeSH Terms

Conditions

Cystic Fibrosis

Condition Hierarchy (Ancestors)

Pancreatic DiseasesDigestive System DiseasesLung DiseasesRespiratory Tract DiseasesGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and AbnormalitiesInfant, Newborn, Diseases

Study Officials

  • Sam Moskowitz MD

    4D Molecular Therapeutics

    STUDY DIRECTOR

Central Study Contacts

4DMT Patient Advocacy

CONTACT

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SEQUENTIAL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

February 10, 2022

First Posted

February 21, 2022

Study Start

March 29, 2022

Primary Completion (Estimated)

June 1, 2027

Study Completion (Estimated)

June 1, 2031

Last Updated

March 11, 2026

Record last verified: 2026-03

Locations