NCT05219162

Brief Summary

Although some small sample studies have reported the possible resistance mechanisms of Osimertinib in the first-line treatment, it is still an urgent need to explore the whole gene profile in EGFRm advanced NSCLC patients post Osimertinib 1L treatment by paired tissue and plasma to guide subsequent treatment strategy. Thus, the gene profile post Osimertinib 1L treatment in tissue and plasma may help to guide the following treatment. Participants will be required to provide paired tissue and whole blood after disease progression following 1L Osimertinib. 200 tissue samples and 200 whole blood samples will be used to detect gene alteration by NGS, respectively. 200 tissue samples will be used to detect pathological transformation by IHC. Approximately 80-100 tissue samples will be used to test MET overexpression by MET IHC and MET amplification by FISH respectively. Approximately 80-100 whole blood samples will be used to test MET amplification by ddPCR.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
182

participants targeted

Target at P50-P75 for phase_4

Timeline
Completed

Started Feb 2022

Typical duration for phase_4

Geographic Reach
1 country

16 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

December 21, 2021

Completed
1 month until next milestone

First Posted

Study publicly available on registry

February 1, 2022

Completed
24 days until next milestone

Study Start

First participant enrolled

February 25, 2022

Completed
2.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 29, 2024

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 29, 2024

Completed
1.4 years until next milestone

Results Posted

Study results publicly available

September 11, 2025

Completed
Last Updated

September 11, 2025

Status Verified

August 1, 2025

Enrollment Period

2.2 years

First QC Date

December 21, 2021

Results QC Date

April 15, 2025

Last Update Submit

August 26, 2025

Conditions

Keywords

Advanced or metastatic non-small cell lung cancer

Outcome Measures

Primary Outcomes (1)

  • Percentage of Participants With Gene Alterations inTissue Detected by NGS

    The Percentage of gene alteration detected by NGS(%) = (number of patients with gene alteration detected by NGS)/(total number of patients in the FAS)×100%.

    At enrollment

Secondary Outcomes (6)

  • Percentage of Participants With Gene Alterations in Plasma Detected by NGS

    At enrollment

  • EGFR Sensitivity of Plasma and Tissue

    At enrollment

  • EGFR Specificity of Plasma and Tissue

    At enrollment

  • EGFR PPV of Plasma and Tissue

    At enrollment

  • EGFR NPV of Plasma and Tissue

    At enrollment

  • +1 more secondary outcomes

Study Arms (1)

EGFRm Locally Advanced or Metastatic NSCLC patients post Osimertinib 1L treatment failure

OTHER

participants with advanced or metastatic non-small cell lung cancer, who have already taken osimertinib as a first treatment option, but their cancer has continued to get worse

Genetic: Gene Profile explore

Interventions

The tissue and blood sample for this genetic research will be obtained from the participants at baseline(disease progression after 1L Osimertinib). Paired tissue and whole blood sample should be collected per participant for genetics during the study.

EGFRm Locally Advanced or Metastatic NSCLC patients post Osimertinib 1L treatment failure

Eligibility Criteria

Age18 Years - 86 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Participants are eligible to be included in the study only if all of the following criteria apply:
  • Informed Consent
  • Capable of giving signed informed consent, which includes compliance with the requirements and restrictions listed in the informed consent form (ICF) and in this protocol.
  • Provision of signed and dated, written informed consent form prior to any mandatory study-specific procedures, sampling, and analyses.
  • The ICF process is described in Appendix A3., Sex and Age
  • Male or female, age at least 18 years. Type of Participant and Disease Characteristics
  • Pathologically confirmed non-small cell lung cancer (NSCLC) with documented EGFR sensitive mutation (EGFR 19del and L858R) positive before Osimertinib 1L.
  • Locally advanced (clinical stage IIIB, IIIC) or metastatic NSCLC(clinical stage IVA or IVB) or recurrent NSCLC (per Version 8 of the International Association for the Study of Lung Cancer \[IASLC\] Staging Manual in Thoracic Oncology), not amenable to curative or radiotherapy (e.g., this may occur as systemic recurrence after prior surgery for early stage disease or patients may be newly diagnosed with stage IIIB/IV disease, which is at the start of Osimertinib therapy).
  • Patients must have been treated with Osimertinib as first line therapy until disease progression. Evidence of disease progression following 1L Osimertinib can be confirmed by investigators with criteria in Response Evaluation Criteria in Solid Tumours (RECIST) Version 1.1.
  • Agree to provide adequate tissue and whole blood for testing after disease progression following 1L Osimertinib.
  • Reproduction
  • Female participants who are not abstinent (in line with the preferred and usual lifestyle choice of the participant) and intend to be sexually active with a male partner must be using highly effective contraceptive measures, must not be breast feeding, and must have a negative pregnancy test prior to the enrolment or must have evidence of non-child-bearing potential by fulfilling 1 of the following criteria at screening:
  • Post-menopausal, defined as more than 50 years of age and amenorrhoeic for at least 12 months following cessation of all exogenous hormonal treatments
  • Women under 50 years old would be considered as postmenopausal if they have been amenorrhoeic for 12 months or more following cessation of exogenous hormonal treatments and have luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels in the post-menopausal range for the institution
  • Documentation of irreversible surgical sterilization by hysterectomy, bilateral oophorectomy or bilateral salpingectomy but not tubal ligation.
  • +1 more criteria

You may not qualify if:

  • Participants are excluded from the study if any of the following criteria apply:
  • Medical Conditions
  • Any concurrent and/or other active malignancy that may affect tissue or whole blood testing results.
  • As judged by the investigator, any evidence of severe or uncontrolled systemic diseases, which in the investigator's opinion, makes it undesirable for the participant to participate in the study or that would jeopardise compliance with the protocol,. Screening for chronic conditions is not required.
  • Prior/Concomitant Therapy
  • Any concurrent anticancer treatment except local radiotherapy and radiotherapy for CNS metastasis. Concurrent use of hormonal therapy for non cancer related conditions (eg, hormone replacement therapy) is allowed.
  • Prior/Concurrent Clinical Study Experience 5 Judgment by the investigator that the patient should not participate in the study if the patient is unlikely to comply with study procedures, restrictions and requirements.
  • Prior allogeneic bone marrow transplant
  • Non-leukocyte depleted whole blood transfusion within 120 days of genetic sample collection.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (16)

Research Site

Beijing, 100210, China

Location

Research Site

Changsha, 410013, China

Location

Research Site

Chengdu, 610014, China

Location

Research Site

Dalian, 116023, China

Location

Research Site

Foshan, 528000, China

Location

Research Site

Fuzhou, 350014, China

Location

Research Site

Harbin, 150081, China

Location

Research Site

Hefei, 230001, China

Location

Research Site

Hefei, 230601, China

Location

Research Site

Linhai, 317000, China

Location

Research Site

Luoyang, 471003, China

Location

Research Site

Rizhao, 276826, China

Location

Research Site

Shanghai, 200032, China

Location

Research Site

Taizhou, 225300, China

Location

Research Site

Tianjin, 300060, China

Location

Research Site

Zhengzhou, 450000, China

Location

Related Links

MeSH Terms

Conditions

Carcinoma, Non-Small-Cell Lung

Condition Hierarchy (Ancestors)

Carcinoma, BronchogenicBronchial NeoplasmsLung NeoplasmsRespiratory Tract NeoplasmsThoracic NeoplasmsNeoplasms by SiteNeoplasmsLung DiseasesRespiratory Tract Diseases

Results Point of Contact

Title
Global Clinical Lead
Organization
AstraZeneca

Study Officials

  • Yuankai Shi, PhD

    Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 4
Allocation
NA
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Model Details: Participants will be required to provide paired tissue and whole blood after disease progression following 1L Osimertinib. 200 tissue samples and 200 whole blood samples will be used to detect gene alteration by NGS, respectively. 200 tissue samples will be used to detect pathological transformation by IHC. Approximately 80-100 tissue samples will be used to test MET overexpression by MET IHC and MET amplification by FISH respectively. Approximately 80-100 whole blood samples will be used to test MET amplification by ddPCR.
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 21, 2021

First Posted

February 1, 2022

Study Start

February 25, 2022

Primary Completion

April 29, 2024

Study Completion

April 29, 2024

Last Updated

September 11, 2025

Results First Posted

September 11, 2025

Record last verified: 2025-08

Data Sharing

IPD Sharing
Will share

Qualified researchers can request access to anonymized individual patient-level data from AstraZeneca group of companies sponsored clinical trials via the request portal. All request will be evaluated as per the AZ disclosure commitment: https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure. Yes, indicates that AZ are accepting requests for IPD, but this does not mean all requests will be shared.

Time Frame
AstraZeneca will meet or exceed data availability as per the commitments made to the EFPIA Pharma Data Sharing Principles. For details of our timelines, please rerefer to our disclosure commitment at https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure
Access Criteria
When a request has been approved AstraZeneca will provide access to the deidentified individual patient-level data in an approved sponsored tool . Signed Data Sharing Agreement (non-negotiable contract for data accessors) must be in place before accessing requested information. Additionally, all users will need to accept the terms and conditions of the SAS MSE to gain access. For additional details, please review the Disclosure Statements at https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure.
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