Biomarkers of Atrial Fibrillation Complicated With Stroke Based on Proteomics and Metabolomics

NCT05181787 | Unknown

• 1 other identifier: AF and stroke 1
• Study Type: observational
• Target: 200
• Locations: 1 country
• Sites: 1

Brief Summary

Thrombus shedding in patients with atrial fibrillation (AF) can lead to cerebral artery embolism. Stroke caused by AF is very dangerous, which not only threatens the life of patients, but also seriously affects the quality of life. This study aims to explore the biomarkers of stroke in patients with AF by integrating proteomics and metabolomics data, and establish the network relationship of stroke in patients with AF, so as to reveal the molecular mechanism of stroke in patients with AF.

Conditions

Atrial Fibrillation; Stroke

Keywords

Atrial Fibrillation; Stroke; metabolomics; proteomics; Biomarkers

Outcome Measures

Primary Outcomes (1)

• Biomarkers of atrial fibrillation complicated with stroke based on proteomics and metabolomics

  By combining proteomics and metabolomics data, the investigators were able to identify the common pathways involved in AF with stroke.

  1 day

Secondary Outcomes (3)

• Quantitative changes of individual metabolites and proteins were calculated and graphed using the KEGG mapping tools.

  1 day

• By combining proteomics and metabolomics data, the investigators were able to identify the common pathways involved in AF with stroke.

  1 day

• All of the omics data were used to compare proteins/enzymes with metabolic pathways.

  1 day

Study Arms (2)

AF

Patients with AF

Diagnostic Test: Proteomics; Diagnostic Test: Metabolomics

AF and stroke

Patients with AF complicated with stroke

Diagnostic Test: Proteomics; Diagnostic Test: Metabolomics

Interventions

Proteomics DIAGNOSTIC_TEST

Proteomic data were obtained by LC-MS/MS Analysis-DIA platform.

AF; AF and stroke

Metabolomics DIAGNOSTIC_TEST

Metabolomic data were obtained by UPLC-QTOF/MS platform.

AF; AF and stroke

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The blood samples of patients with AF and patients with AF complicated with stroke were taken. The supernatant was centrifuged and put into - 80 ℃ refrigerator for storage.

You may qualify if:

• Age 18 years or above
• Admission with atrial fibrillation or clinic visit for atrial fibrillation
• Receive routine anticoagulant therapy;
• Signing the consent form

You may not qualify if:

• Pregnant women;
• Lactating women;
• Severe mitral stenosis;
• Severe impairment of liver function;
• Severe renal insufficiency;
• Thyroid dysfunction requiring treatment.

Sponsors & Collaborators

• Yue LI: lead

Study Sites (1)

Yan

Harbin, Heilongjiang, 150001, China

RECRUITING

MeSH Terms

Conditions

Atrial Fibrillation; Stroke

Condition Hierarchy (Ancestors)

Arrhythmias, Cardiac; Heart Diseases; Cardiovascular Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Cerebrovascular Disorders; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Vascular Diseases

Central Study Contacts

Haiyu Zhang, MD

CONTACT

86-451-85555009; zhanghaiyu819@163.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Target Duration: 1 Day
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: December 20, 2021
• First Posted: January 6, 2022
• Study Start: December 20, 2021
• Primary Completion: December 31, 2023
• Study Completion: December 31, 2023
• Last Updated: January 25, 2022

Record last verified: 2022-01

Locations

CN (1)