NCT05117125

Brief Summary

The purpose of this study is to evaluate different peptide biomarkers, variations in the microbiome and patterns in the bacterial transcriptome as prognostic or diagnostic biomarkers of VAP.

Trial Health

80
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,000

participants targeted

Target at P75+ for all trials

Timeline
32mo left

Started Oct 2021

Longer than P75 for all trials

Geographic Reach
3 countries

5 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress63%
Oct 2021Dec 2028

First Submitted

Initial submission to the registry

October 15, 2021

Completed
Same day until next milestone

Study Start

First participant enrolled

October 15, 2021

Completed
27 days until next milestone

First Posted

Study publicly available on registry

November 11, 2021

Completed
5.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
2 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2028

Last Updated

November 15, 2024

Status Verified

November 1, 2024

Enrollment Period

5.2 years

First QC Date

October 15, 2021

Last Update Submit

November 13, 2024

Conditions

Keywords

VAP

Outcome Measures

Primary Outcomes (4)

  • Change in HBP concentration over time

    Change in concentration (ng/ml) compared to baseline.

    Day 1, 3 and in selected patients day 7 and 14. In patients with VAP also VAP day 1, 3 and 7.

  • Change in IL-26 over time

    Change in concentration (ng/ml) compared to baseline.

    Day 1, 3 and in selected patients day 7 and 14. In patients with VAP also VAP day 1, 3 and 7.

  • HBP at VAP diagnosis

    Concentration at VAP day 1, compared to day 3 in patients with no VAP. ROC curves, specificity and sensitivity as diagnostic biomarker.

    VAP day 1 compared to No VAP day 3

  • IL-26 at VAP diagnosis

    Concentration at VAP day 1, compared to day 3 in patients with no VAP. ROC curves, specificity and sensitivity as diagnostic biomarker.

    VAP day 1 compared to No VAP day 3

Secondary Outcomes (2)

  • Diversity of microbiome

    Day 1 and 3, and VAP day 1.

  • Bacterial transcriptome patterns

    VAP day 1

Other Outcomes (9)

  • Diagnostic certainty of VAP

    At VAP day 1

  • Change in CRP concentration

    Day 1, 3 and in selected patients day 7 and 14. In patients with VAP also VAP day 1, 3 and 7.

  • Change in Differential blood cell counts

    Day 1, 3 and in selected patients day 7 and 14. In patients with VAP also VAP day 1, 3 and 7.

  • +6 more other outcomes

Study Arms (3)

VAP

Enrolled participants that fully meet the criteria of VAP.

Diagnostic Test: Heparin-binding proteinDiagnostic Test: Interleukin-26Diagnostic Test: MicrobiomeDiagnostic Test: Bacterial transcriptomeDiagnostic Test: Proteome

Suspect VAP

Enrolled participants that develop signs of VAP but lack some variable, for instace new radiographic infiltrate or microbiological finding compatible with VAP.

Diagnostic Test: Heparin-binding proteinDiagnostic Test: Interleukin-26Diagnostic Test: MicrobiomeDiagnostic Test: Proteome

No VAP

Enrolled participants that do not develop VAP.

Diagnostic Test: Heparin-binding proteinDiagnostic Test: Interleukin-26Diagnostic Test: MicrobiomeDiagnostic Test: Proteome

Interventions

Evaluation of the specificity and sensitivity of HBP as diagnostic biomarker for VAP.

Also known as: HBP
No VAPSuspect VAPVAP
Interleukin-26DIAGNOSTIC_TEST

Evaluation of the specificity and sensitivity of IL-26 as diagnostic biomarker for VAP.

Also known as: IL-26
No VAPSuspect VAPVAP
MicrobiomeDIAGNOSTIC_TEST

Evaluation of the specificity and sensitivity of the lung microbiome as prognostic biomarker for VAP.

No VAPSuspect VAPVAP

Evaluation of the specificity and sensitivity of the bacterial transcriptome as prognostic biomarker for treatment failure of VAP.

VAP
ProteomeDIAGNOSTIC_TEST

Evaluation of the specificity and sensitivity of the proteome in mini-BAL as biomarker for VAP.

No VAPSuspect VAPVAP

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

General intensive care unit population.

You may qualify if:

  • Admission to an intensive care unit
  • Intubation within last 24 hours
  • Anticipated mechanical ventilation of at least 48 hours

You may not qualify if:

  • FiO2 above 70% or PEEP above 15
  • Ongoing infection of the lungs at admission to the ICU.
  • Severely elevated or instable intracranial pressure.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (5)

Vestre Viken

Drammen, 3004, Norway

RECRUITING

Hospital de São Francisco Xavier

Lisbon, Portugal

RECRUITING

Skåne University Hospital, Dept. of Infectious diseases

Lund, Sweden

RECRUITING

Skåne university Hospital, ICU

Malmo, Sweden

RECRUITING

Karolinska University Hospital, ICU

Solna, Sweden

NOT YET RECRUITING

Related Publications (2)

  • Paulsson M, Cardenas EI, Che KF, Brundin B, Smith M, Qvarfordt I, Linden A. TLR4-mediated release of heparin-binding protein in human airways: a co-stimulatory role for IL-26. Front Immunol. 2023 May 10;14:1178135. doi: 10.3389/fimmu.2023.1178135. eCollection 2023.

    PMID: 37234157BACKGROUND
  • Paulsson M, Thelaus L, Riesbeck K, Qvarfordt I, Smith ME, Linden A, Linder A. Heparin-binding protein in lower airway samples as a biomarker for pneumonia. Respir Res. 2021 Jun 8;22(1):174. doi: 10.1186/s12931-021-01764-2.

    PMID: 34103069BACKGROUND

Related Links

Biospecimen

Retention: SAMPLES WITH DNA

Mini-BAL Plasma Protected specimen brushes

MeSH Terms

Conditions

Pneumonia, Ventilator-Associated

Interventions

Microbiota

Condition Hierarchy (Ancestors)

Healthcare-Associated PneumoniaCross InfectionInfectionsPneumoniaRespiratory Tract InfectionsLung DiseasesRespiratory Tract DiseasesIatrogenic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Microbiological PhenomenaBiotaBiodiversityEcosystemEnvironmentEcological and Environmental PhenomenaBiological PhenomenaEnvironment and Public Health

Study Officials

  • Magnus Paulsson, PhD MD

    Skane University Hospital

    PRINCIPAL INVESTIGATOR
  • Fredrik Sjövall, PhD MD

    Skane University Hospital

    STUDY CHAIR

Central Study Contacts

Magnus Paulsson, PhD MD

CONTACT

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 15, 2021

First Posted

November 11, 2021

Study Start

October 15, 2021

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

December 31, 2028

Last Updated

November 15, 2024

Record last verified: 2024-11

Data Sharing

IPD Sharing
Will share

Individual participant data that underlie the results reported in published articles will be shared with investigators after deidentification upon request.

Shared Documents
STUDY PROTOCOL
Time Frame
IPD will be available from 6 months to 5 years after publication.
Access Criteria
Approval of the relevant ethical board is necessary for access

Locations