NCT05081154

Brief Summary

This study is a single-center, open-label, randomized, 7-way crossover study designed to evaluate plasma nicotine pharmacokinetics (PK) parameters following use of the study investigational products (IPs) in a confinement setting by generally healthy combustible cigarette (CC) smokers.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
36

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Oct 2021

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

October 4, 2021

Completed
14 days until next milestone

First Posted

Study publicly available on registry

October 18, 2021

Completed
2 days until next milestone

Study Start

First participant enrolled

October 20, 2021

Completed
1 month until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 24, 2021

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 24, 2021

Completed
Last Updated

June 27, 2022

Status Verified

June 1, 2022

Enrollment Period

1 month

First QC Date

October 4, 2021

Last Update Submit

June 23, 2022

Conditions

Outcome Measures

Primary Outcomes (2)

  • AUC nic 0-240

    Baseline-adjusted area under the plasma nicotine concentration-versus-time curve from time zero to 240 minutes after the start of IP use

    0 to 240 minutes

  • Cmax

    Maximum baseline-adjusted plasma concentration of nicotine

    240 minutes

Study Arms (14)

Product usage order A B G C F D E

EXPERIMENTAL

Subjects will use each of the 7 products (A B G C F D E) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order B C A D G E F

EXPERIMENTAL

Subjects will use each of the 7 products (B C A D G E F) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order C D B E A F G

EXPERIMENTAL

Subjects will use each of the 7 products (C D B E A F G) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order D E C F B G A

EXPERIMENTAL

Subjects will use each of the 7 products (D E C F B G A) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order E F D G C A B

EXPERIMENTAL

Subjects will use each of the 7 products (E F D G C A B) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order F G E A D B C

EXPERIMENTAL

Subjects will use each of the 7 products (F G E A D B C) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order G A F B E C D

EXPERIMENTAL

Subjects will use each of the 7 products (G A F B E C D) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order E D F C G B A

EXPERIMENTAL

Subjects will use each of the 7 products (E D F C G B A) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order F E G D A C B

EXPERIMENTAL

Subjects will use each of the 7 products (F E G D A C B) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order G F A E B D C

EXPERIMENTAL

Subjects will use each of the 7 products (G F A E B D C) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order A G B F C E D

EXPERIMENTAL

Subjects will use each of the 7 products (A G B F C E D) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order B A C G D F E

EXPERIMENTAL

Subjects will use each of the 7 products (B A C G D F E) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order C B D A E G F

EXPERIMENTAL

Subjects will use each of the 7 products (C B D A E G F) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Product usage order D C E B F A G

EXPERIMENTAL

Subjects will use each of the 7 products (D C E B F A G) during an evaluation period, followed by a 4 hour Test Session

Other: Product AOther: Product BOther: Product COther: Product DOther: Product EOther: Product FOther: Product G

Interventions

P1012915, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1013018, a nicotine pouch with 10 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1013015, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1012815, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1013315, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1013115, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

P1013215, a nicotine pouch with 8 mg nicotine

Product usage order A B G C F D EProduct usage order A G B F C E DProduct usage order B A C G D F EProduct usage order B C A D G E FProduct usage order C B D A E G FProduct usage order C D B E A F GProduct usage order D C E B F A GProduct usage order D E C F B G AProduct usage order E D F C G B AProduct usage order E F D G C A BProduct usage order F E G D A C BProduct usage order F G E A D B CProduct usage order G A F B E C DProduct usage order G F A E B D C

Eligibility Criteria

Age21 Years - 60 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Able to read, understand, and willing to sign an informed consent form (ICF) and complete questionnaires written in English.
  • Generally healthy males or females, 21 to 60 years of age, inclusive, at the time of consent.
  • Smokes combustible, filtered, non-menthol or menthol cigarettes, 83 mm to 100 mm in length as primary source of tobacco.
  • Smokers who also use ST products (e.g., moist snuff, snus), and have used ST within 30 days prior to screening will be enrolled.
  • Smokes an average of at least 10 cigarettes per day (CPD) and inhale the smoke, for at least 6 months prior to Screening. Brief periods of abstinence due to illness, quit attempt (prior to 30 days of Screening), or clinical study participation (prior to 30 days of Screening) will be allowed at the discretion of the PI.
  • Agrees to smoke the same Usual Brand (UB) cigarette throughout the study period. The UB cigarette is defined as the reported cigarette brand and style currently smoked most frequently by the subject.
  • Expired breath carbon monoxide (ECO) level is ≥ 10 ppm and ≤ 100 ppm at Screening and at check-in Day 1.
  • Positive urine cotinine test at Screening.
  • Response at Screening to the Fagerström Test for Nicotine Dependence (FTND) Question 1 ("How soon after you wake up do you smoke your first cigarette?") is either "Within 5 minutes" or "6-30 minutes" (Heatherton et al., 1991).
  • Willing to use the UB cigarette and Velo Pouch IPs during the study period.
  • Willing to abstain from tobacco and nicotine use for at least 12 hours prior to the start of each of seven Test Sessions.
  • Females must be willing to use a form of contraception acceptable to the PI from the time of signing the informed consent until End-of-Study.
  • Examples of acceptable means of birth control are, but not limited to: a) Surgical sterilization (hysterectomy, bilateral tubal ligation/occlusion, bilateral oophorectomy, bilateral salpingectomy); b) physical barrier method (e.g., condom, diaphragm/sponge/cervical cap) with spermicide; c) non-hormone releasing intrauterine devices (IUD) or hormone-releasing IUDs (e.g., Mirena or Kyleena); d) vasectomized partner; and e) post-menopausal and not on hormone replacement therapy.
  • Agrees to an in-clinic confinement of 8 days (7 nights).

You may not qualify if:

  • Presence of clinically significant uncontrolled cardiovascular, pulmonary, renal, hepatic, endocrine, gastrointestinal, psychiatric, hematological, neurological disease, or any other concurrent disease or medical condition that, in the opinion of the PI, makes the study subject unsuitable to participate in this clinical study.
  • History, presence of, or clinical laboratory test results indicating diabetes.
  • Scheduled treatment for asthma currently or within the past consecutive 12 months prior to the Screening Visit. As-needed treatment, such as inhalers, may be included at the PI's discretion pending approval from the Medical Monitor.
  • History or presence of bleeding or clotting disorders.
  • Any history of cancer, except for primary cancers of skin such as localized basal cell/squamous cell carcinoma that has been surgically and/or cryogenically removed.
  • Systolic blood pressure of \> 160 mmHg or a diastolic blood pressure of \> 95 mmHg, measured after being seated for five minutes at Screening and at check-in Day 1.
  • Weight of ≤ 110 pounds.
  • Hemoglobin level is \< 12.5 g/dL for females or \<13.0 g/dL for males at Screening.
  • Females who have a positive pregnancy test, are pregnant, breastfeeding, or intend to become pregnant during the course of the study.
  • A positive urine drug screen without evidence of prescribed corresponding concomitant medication(s) at Screening or check-in Day 1.
  • Positive test for human immunodeficiency virus (HIV), hepatitis B surface antigen (HBsAg), or hepatitis C virus (HCV).
  • Use of any medication or substance that aids in smoking cessation, including but not limited to any nicotine replacement therapy (e.g., nicotine gum, lozenge, patch), varenicline (Chantix®), bupropion (Wellbutrin®, Zyban®), or lobelia extract within (≤) 30 days prior to the signing of informed consent.
  • Postpones a decision to quit using tobacco- or nicotine-containing products in order to participate in this study or self-reports a previous quit attempt within (≤) 30 days prior to the signing the informed consent.
  • Any use of daily aspirin (≥ 325 mg/day) or any use of other anticoagulants.
  • Individuals ≥ 35 years of age currently using systemic, estrogen-containing contraception or hormone replacement therapy.
  • +7 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

AMR Knoxville

Knoxville, Tennessee, 37920, United States

Location

Related Publications (1)

  • Kanobe MN, Powell CY, Patrudu M, Baxter SA, Tapia MA, Darnell J, Prevette K, Gibson AG, Ayoku SA, Campbell L, Coffield JW, Keyser BM, Ganesh BS, Gale N, Jordan KG. Randomized crossover clinical studies to assess abuse liability and nicotine pharmacokinetics of Velo Oral Nicotine pouches. Front Pharmacol. 2025 Mar 13;16:1547073. doi: 10.3389/fphar.2025.1547073. eCollection 2025.

MeSH Terms

Conditions

SmokingTobacco Use

Interventions

HTR3D protein, human

Condition Hierarchy (Ancestors)

Behavior

Study Officials

  • Milly Kanobe, PhD

    RAIS

    STUDY DIRECTOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
OTHER
Intervention Model
CROSSOVER
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 4, 2021

First Posted

October 18, 2021

Study Start

October 20, 2021

Primary Completion

November 24, 2021

Study Completion

November 24, 2021

Last Updated

June 27, 2022

Record last verified: 2022-06

Data Sharing

IPD Sharing
Will not share

Locations