NCT04985994

Brief Summary

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is a deadly infectious disease and major global public health problem. Recent evidence from animal studies suggests that the microbiome plays a role in TB pathogenesis and immune response. However, until now, no similar study has been performed in humans and thus any influence of the microbiota on TB or vice versa remains unknown.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
450

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Aug 2021

Typical duration for all trials

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 22, 2021

Completed
11 days until next milestone

First Posted

Study publicly available on registry

August 2, 2021

Completed
20 days until next milestone

Study Start

First participant enrolled

August 22, 2021

Completed
2.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 31, 2024

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

August 31, 2024

Completed
Last Updated

October 10, 2024

Status Verified

October 1, 2024

Enrollment Period

2.6 years

First QC Date

July 22, 2021

Last Update Submit

October 9, 2024

Conditions

Keywords

TuberculosisPulmonary TBMicrobiomeImmunityNutritionTreatment responseTreatment OutcomeGut microbiomeOral MicrobiomeInflammationDietary intake

Outcome Measures

Primary Outcomes (1)

  • Gut microbiome diversity and functional potential

    To explore the effect of TB infection and anti-tuberculosis therapy on gut microbiome diversity, functional potential and immune response in newly diagnosed TB patients from Pakistan.

    2 Years

Secondary Outcomes (5)

  • Baseline Gut microbiome

    1 year

  • Baseline Oral microbiome

    1 year

  • Gut microbiome and associated factors

    1 year

  • Microbial dysbiosis

    Two years

  • Microbial signatures

    2 years

Study Arms (2)

Tuberculosis Patients

1. Diagnosed with pulmonary TB after detailed history collection, clinical examination, and laboratory assessment (sputum culture positive). 2. Aged 18 years or above. 3. Willing to participate in the study.

Healthy Volunteers

1. Healthy subjects with no symptoms or history of pulmonary TB 2. Negative sputum culture 3. Matched for sex and age (±5 years) with the TB patient group.

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

The study will be conducted primarily in three district levels (Peshawar, Mardan and Swat) TB diagnostic and treatment centers of Khyber Pakhtunkhwa province of Pakistan. These centers have been selected based on the highest TB prevalence in the year 2020

You may qualify if:

  • Diagnosed with TB after detailed history collection, clinical examination and laboratory assessment (sputum culture positive).
  • Aged 18 - 65 years.
  • Willing to participate in the study.
  • Healthy controls are those who are free of TB symptoms, healthy on physical examination and with a negative sputum culture result.

You may not qualify if:

  • Already on anti-TB treatment or previously treated for TB.
  • Severely anemic (Hb \< 10g/dL).
  • Having diarrhea or other major gastrointestinal disorders.
  • Using or have used aminoglycoside or quinolones antibiotics in the past one month.
  • Using a medically prescribed diet or nutrition supplement.
  • Pregnant or lactating women.
  • Patients with liver or renal dysfunction, or having any other chronic disease condition.
  • Multidrug resistance TB patients

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

DTO Peshawar

Peshawar, Khyber Pakhtunkhwa, 23061, Pakistan

Location

Khyber Medical University

Peshawar, Khyber Pakhtunkhwa, 25110, Pakistan

Location

Related Publications (1)

  • Shahzad M, Andrews SC, Ul-Haq Z. Exploring the role of Microbiome in Susceptibility, Treatment Response and Outcome among Tuberculosis Patients from Pakistan: study protocol for a prospective cohort study (Micro-STOP). BMJ Open. 2022 Jun 7;12(6):e058463. doi: 10.1136/bmjopen-2021-058463.

Biospecimen

Retention: SAMPLES WITH DNA

Stool, Whole Blood, Salivary rinse

MeSH Terms

Conditions

TuberculosisTuberculosis, PulmonaryInflammation

Condition Hierarchy (Ancestors)

Mycobacterium InfectionsActinomycetales InfectionsGram-Positive Bacterial InfectionsBacterial InfectionsBacterial Infections and MycosesInfectionsRespiratory Tract InfectionsLung DiseasesRespiratory Tract DiseasesPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • Muhammad Shahzad

    Khyber Medical University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

July 22, 2021

First Posted

August 2, 2021

Study Start

August 22, 2021

Primary Completion

March 31, 2024

Study Completion

August 31, 2024

Last Updated

October 10, 2024

Record last verified: 2024-10

Data Sharing

IPD Sharing
Will share

Access to the trial IPD can be requested by qualified researchers with research or academic interests in Tuberculosis or Microbiome research. The individual researchers will be required to send an email to the principal investigator describing the need and rationale for IPD. However, the access will be provided only after the proposal and statistical analysis plan is reviewed by the principal investigator and ORIC office of Khyber Medical University. The party requesting the access will also be required to sign Data Sharing agreement and or Material transfer agreement. Before sharing, the principal investigator will make sure that the samples and data are coded and all the personal information are removed.

Shared Documents
STUDY PROTOCOL, SAP
Time Frame
Within one year after the data is published
Access Criteria
The information request should be send directly to the principal investigator by email.
More information

Available IPD Datasets

Study Protocol Access

Locations