RNA-seq Analysis of PBMC in Patients With OPLL

NCT04963101 | Unknown

• 1 other identifier: ZDWY.CSYGJWK.003
• Study Type: observational
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

In this study, we planned to identify transcriptome expression profiles (circRNAs, lncRNAs, and mRNAs) from peripheral blood mononuclear cells (PBMCs) of 30 patients with OPLL and 30 other non-OPLL patients by high-throughput sequencing.Sequence analysis was used to predict miRNAs that might bind circRNAs and lncRNAs.Through bioinformatics analysis, competitive endogenous RNAs (cerRNAs), as well as coexpression networks among circRNAs, lncRNAs, miRNAs, and mRNAs, were constructed to provide key molecules and signaling pathways associated with OPLL.

Conditions

OPLL; PBMCs; Transcriptome; Bioinformatics

Outcome Measures

Primary Outcomes (1)

• circRNA, lncRNA, mRNA

  1 year

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

A total of 30 peripheral blood samples were enrolled in this study, including 15 cases in the OPLL disease group and 15 cases in the non-OPLL control group.

You may qualify if:

• Voluntarily participate in the clinical study and sign the informed consent;
• All OPLL patients meet the diagnostic criteria set out in the JOA Clinical Practice Guidelines for Spinal Ligament Osification 2019;
• Age ≥18, gender unlimited;
• All non-OPLL patients and healthy volunteers have no serious diseases of the heart, lung, brain, liver, kidney and blood system, such as autoimmune diseases, tumors, psychosis, infectious diseases, etc., and have no bad habits or recent history of taking special drugs.

You may not qualify if:

• Unable to sign the informed consent;
• \<18 years old;
• Women planning to become pregnant/lactating/pregnant;
• Complicated with serious heart, lung, brain, liver, kidney, blood system diseases, such as autoimmune diseases, tumors, mental illness, infectious diseases, drug addiction and other bad addicts;Those who have recently taken special drugs;
• Individuals who do not agree to donate peripheral blood or who are obviously uncomfortable with drawing blood.

Sponsors & Collaborators

• Fifth Affiliated Hospital, Sun Yat-Sen University: lead

Study Sites (1)

Facility

Zhuhai, Guangdong, China

RECRUITING

Central Study Contacts

Ning Jiang, M.D

CONTACT

+86 15653868023; jiangn57@mail2.sysu.edu.cn

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Director of orthopaedics

Study Record Dates

• First Submitted: July 2, 2021
• First Posted: July 15, 2021
• Study Start: June 25, 2021
• Primary Completion: June 25, 2022
• Study Completion: June 30, 2022
• Last Updated: July 15, 2021

Record last verified: 2021-07

Locations

CN (1)