NCT04962893

Brief Summary

This is a randomized, parallel dose assigned, double blind, multi center, Phase II study assessing the efficacy, safety, and immunogenicity of VLP vaccine (Authentic and Alpha variants) in adults between 18 and 59 years who are healthy or have medically stable chronic diseases and who have no known history of SARS-CoV-2 infection

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
349

participants targeted

Target at P75+ for phase_2 covid19

Timeline
Completed

Started Jun 2021

Geographic Reach
1 country

3 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 26, 2021

Completed
16 days until next milestone

First Submitted

Initial submission to the registry

July 12, 2021

Completed
3 days until next milestone

First Posted

Study publicly available on registry

July 15, 2021

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 16, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 16, 2022

Completed
Last Updated

May 31, 2022

Status Verified

May 1, 2022

Enrollment Period

7 months

First QC Date

July 12, 2021

Last Update Submit

May 24, 2022

Conditions

Keywords

Virus-like Particles harboring S, M, N, E antigensSARS-CoV-2K3-CpG ODNAlum

Outcome Measures

Primary Outcomes (7)

  • Comparison of efficacy

    Comparison of antibody responses of participants to a cohort of standard convalescent serum samples obtained from World Health Organization (WHO).

    On Day 14 after booster dose administration

  • Comparison of efficacy

    Comparison of antibody responses of participants to a cohort of standard convalescent serum samples obtained from World Health Organization (WHO).

    On Day 28 after booster dose administration

  • Specific antibody (IgG) response

    SARS-CoV-2 Spike/S1 or RBD antibody titers

    On Day 14 after booster dose administration

  • Specific antibody (IgG) response

    SARS-CoV-2 Spike/S1 or RBD antibody titers

    On Day 28 after booster dose administration

  • Neutralizing antibody response

    Neutralizing antibody titer against anti-Spike protein by virus neutralization method developed against SARS-CoV-2

    On Day 14 after booster dose administration

  • Neutralizing antibody response

    Neutralizing antibody titer against anti-Spike protein by virus neutralization method developed against SARS-CoV-2

    On Day 28 after booster dose administration

  • Cellular immune response

    ELISPOT: Interferon-γ (IFN-γ) positive level of T-cells

    Before first dose administration, on Day 14 after booster dose administration

Secondary Outcomes (3)

  • Adverse events (AEs)

    Until Month 12 after booster dose administration

  • Serious adverse events (SAEs)

    Until Month 12 after booster dose administration

  • Specific antibody (IgG) response

    Before first and booster dose administration, at Month 3, Month 6, Month 9 and Month 12 after booster dose

Study Arms (3)

VLP-Wuhan group (Group V1)

EXPERIMENTAL

110 participants will receive 40 mcg of Alum adsorbed VLP vaccine for Wuhan adjuvanted with K3-CpGODN (1 ml), in two doses 21 days apart.

Biological: SARS-CoV-2 VLP Vaccine-Wuhan

VLP-Alpha (British) variant group (Group V2)

EXPERIMENTAL

110 participants will receive 40 mcg of Alum adsorbed VLP vaccine for Alpha variant adjuvanted with K3-CpGODN (1 ml), in two doses 21 days apart.

Biological: SARS-CoV-2 VLP Vaccine-Alpha (British) variant

VLP-Wuhan+Alpha group (Group V3)

EXPERIMENTAL

110 participants will receive 40 mcg of Alum adsorbed VLP vaccine for Wuhan and Alpha variant adjuvanted with K3-CpGODN (1 ml), in two doses 21 days apart. Initial vaccination with Wuhan followed by a booster of Alpha variant.

Biological: SARS-CoV-2 VLP Vaccine-Wuhan+Alpha variant

Interventions

Alum adsorbed, CpG ODN adjuvanted VLP vaccine expressing HexaPro-S, M, N, E proteins of the virus

Also known as: Authentic VLP Vaccine
VLP-Wuhan group (Group V1)

Alum adsorbed, CpG ODN adjuvanted VLP vaccine expressing HexaPro-S, M, N, E proteins of the virus

Also known as: Alpha Variant VLP Vaccine
VLP-Alpha (British) variant group (Group V2)

Alum adsorbed, CpG ODN adjuvanted VLP vaccine expressing HexaPro-S, M, N, E proteins of the Wuhan or Alpha variants

Also known as: Combination of Authentic and Alpha VLP Vaccine
VLP-Wuhan+Alpha group (Group V3)

Eligibility Criteria

Age18 Years - 59 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • To be eligible for the study, each participant must satisfy all the following criteria:
  • Female and/or male participant who is informed and about his/her participation and who agrees to give his/her written informed consent.
  • Aged between 18 and 59 years.
  • Negative Immunoglobulin G (IgG)/Immunoglobulin M (IgM) antibody for COVID-19.
  • Negative COVID-19 quantitative polymerase chain reaction (qPCR) test result.
  • Able to comply with all scheduled visits, vaccination plan, laboratory tests, lifestyle considerations, and other study procedures.
  • Negative blood test for hepatitis B (HBV), hepatitis C (HCV) and human immunodeficiency virus (HIV) at screening period.
  • Body temperature \< 37.2°C.
  • Body Mass Index (BMI) ranged between 18-35 kg/m2.
  • Clinical laboratory test results within the reference range of the laboratory or clinically non-significant (complete blood count (CBC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, urea, creatinine, and fasting glucose) or any laboratory parameters defined in the study protocol.
  • Good general health as determined by physical examination, laboratory screening, and review of medical history within 14 days prior to participation.
  • Female participants of childbearing potential may be enrolled in the study if the subject fulfils all the following criteria:
  • Have a negative pregnancy test on the day of screening and prior to each study vaccine administration.
  • Use an effective contraceptive method for at least 30 days prior to first dose of study vaccine and agree to continue using one highly effective form of birth control through 6 months after the administration of the last dose of study vaccine.
  • Female participants of non-childbearing potential may be enrolled in the study. Non-childbearing potential is defined as postmenopausal (defined as amenorrhea for ≥12 consecutive months prior to Screening without an alternative medical cause) or surgically sterile (bilateral tubal ligation, bilateral oophorectomy, or hysterectomy).
  • +1 more criteria

You may not qualify if:

  • Participants with any of the following criteria will be excluded:
  • History of laboratory-confirmed SARS-COV-2 infection.
  • History of seizures, encephalopathy, or psychosis.
  • Known or suspected allergy or history of anaphylaxis or other serious adverse reactions to any vaccine or study vaccine and/or any other excipients of the vaccine.
  • Pregnant, breastfeeding or planning to become pregnant within 6 months after the study vaccine administration.
  • Suspected active infection or other acute illness, including fever \> 37.2°C.
  • Any presence of clinical relevance of cardiovascular disease (including but not limited to arrythmia, myocardial infarction, uncontrolled hypertension, coronary artery disease, or congestive heart failure).
  • Any presence of clinical relevance of serious chronic disease \[asthma, diabetes, thyroid diseases etc.).
  • Any presence of clinical relevance of congenital or acquired angioedema.
  • Diagnosis of immunodeficiency.
  • Diagnosis of bleeding diathesis.
  • Use of immunosuppressive medications, anti-allergic therapy, cytotoxic therapy, inhaler corticosteroids (excluding allergic rhinitis or topical steroid ointments).
  • Those who received blood/plasma products or immunoglobulins and/or blood transfusion within the last 6 months.
  • Those who participated in another vaccine study or received an investigational/experimental drug within 1 month prior to study entry.
  • History of any live vaccine within 1 month prior to study participation.
  • +3 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Dr. Abdurahman Yurtaslan Ankara Oncology Training and Research Hospital Phase I Clinical Study Center

Ankara, 06200, Turkey (Türkiye)

Location

Health Sciences University İstanbul Yedikule Chest Diseases and Thoracic Surgery Training and Research Hospital

Istanbul, 34020, Turkey (Türkiye)

Location

Kocaeli University Research and Application Hospital Infectious Disease and Clinical Microbiology Department

Kocaeli, 41380, Turkey (Türkiye)

Location

Related Publications (1)

  • Yilmaz IC, Ipekoglu EM, Golcuklu BS, Bildik T, Aksoy AGB, Evcili I, Turay N, Surucu N, Bulbul A, Guvencli N, Yildirim M, Canavar Yildirim T, Atalay YA, Abras I, Ceylan Y, Ozsurekci Y, Tigen ET, Korten V, Gursel M, Gursel I. A phase I/II study of CpG/alum-adjuvanted mammalian-derived quadruple antigen carrying virus-like particle COVID-19 vaccine. Vaccine. 2025 Mar 7;49:126787. doi: 10.1016/j.vaccine.2025.126787. Epub 2025 Jan 31.

MeSH Terms

Conditions

COVID-19

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Study Officials

  • Fevzi ALTUNTAS

    HEAD OF ONCOLOGY HOSPITAL

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Masking Details
Double-blinded
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Co- Principal Investigator

Study Record Dates

First Submitted

July 12, 2021

First Posted

July 15, 2021

Study Start

June 26, 2021

Primary Completion

January 16, 2022

Study Completion

January 16, 2022

Last Updated

May 31, 2022

Record last verified: 2022-05

Locations