NCT04925206

Brief Summary

This is a single-arm, open label, multi-center, single-dose phase 1 study in subjects with transfusion dependent β-thalassaemia. The study will evaluate the safety and efficacy of autologous CRISPR-Cas9 Modified CD34+ Human Hematopoietic Stem and Progenitor Cells (hHSPCs) using ET-01.

Trial Health

57
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
8

participants targeted

Target at below P25 for phase_1

Timeline
Completed

Started Aug 2021

Typical duration for phase_1

Geographic Reach
1 country

4 active sites

Status
terminated

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 4, 2021

Completed
10 days until next milestone

First Posted

Study publicly available on registry

June 14, 2021

Completed
2 months until next milestone

Study Start

First participant enrolled

August 17, 2021

Completed
2.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 30, 2024

Completed
1.1 years until next milestone

Study Completion

Last participant's last visit for all outcomes

March 12, 2025

Completed
Last Updated

March 30, 2025

Status Verified

March 1, 2025

Enrollment Period

2.5 years

First QC Date

June 4, 2021

Last Update Submit

March 25, 2025

Conditions

Keywords

Beta-ThalassaemiaCRISPR-Cas9Human Hematopoietic Stem and Progenitor Cells

Outcome Measures

Primary Outcomes (5)

  • Frequency and severity of collected AEs & SAEs

    Within 24 months after ET-01 infusion

  • All-cause mortality

    From signing of informed consent up to 24 months post-ET-01 infusion

  • Incidence of transplant-related mortality

    From baseline (pre-transfusion) up to 12 months post-ET-01 infusion

  • Total lymphocyte count

    Within 24 months after ET-01 infusion

  • Proportion of subjects with abnormal proliferation of blood cells

    Within 24 months after ET-01 infusion

Study Arms (1)

ET-01

EXPERIMENTAL
Biological: ET-01

Interventions

ET-01BIOLOGICAL

Recruited participants will receive ET-01 IV infusion following myeloablative conditioning with busulfan.

ET-01

Eligibility Criteria

Age12 Years - 35 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Subjects and/or legal representative fully understand and voluntarily sign informed consent forms.
  • Diagnosis of transfusion dependent β-thalassemia (β-TDT) as defined by protocol.
  • Be not appropriate to receive conventional allogeneic hematopoietic stem cell transplantation.
  • Lansky/Karnofsky score ≥ 70%.
  • Eligible for hematopoietic stem cell transplantation and conditioning with busulfan as per investigator's judgement.

You may not qualify if:

  • Subjects with associated α-thalassemia.
  • Subjects with any clinically significant acute or uncontrolled infections.
  • History of uncontrolled epilepsy or other mental disorders.
  • Previous treatment with allogeneic bone marrow transplantation or gene therapy.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (4)

Nanfang Hospital of Southern Medical University

Guangzhou, Guangdong, 510515, China

Location

Guangzhou Women and Children's Medical Center

Guangzhou, Guangdong, 510623, China

Location

Shenzhen Children's Hospital

Shenzhen, Guangdong, 518035, China

Location

Institute of Hematology & Blood Diseases Hospital

Tianjin, Tianjin Municipality, 300020, China

Location

MeSH Terms

Conditions

beta-Thalassemia

Condition Hierarchy (Ancestors)

ThalassemiaAnemia, Hemolytic, CongenitalAnemia, HemolyticAnemiaHematologic DiseasesHemic and Lymphatic DiseasesHemoglobinopathiesGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and Abnormalities

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 4, 2021

First Posted

June 14, 2021

Study Start

August 17, 2021

Primary Completion

January 30, 2024

Study Completion

March 12, 2025

Last Updated

March 30, 2025

Record last verified: 2025-03

Data Sharing

IPD Sharing
Will not share

Locations