NCT04924491

Brief Summary

The investigators developed a protocol to isolate Treg cells from thymic tissue (thyTreg) discarded in pediatric cardiac surgeries. After completing the pre-clinical studies, the investigators have initiated a phase I/II clinical trial to test the safety and efficacy of the adoptive transfer of autologous thyTreg to prevent rejection in heart transplant children. Condition or disease: Heart Transplantation Intervention/treatment: Regulatory T Cell (Treg) Infusion

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
11

participants targeted

Target at below P25 for phase_1

Timeline
7mo left

Started Sep 2020

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress91%
Sep 2020Dec 2026

Study Start

First participant enrolled

September 10, 2020

Completed
8 months until next milestone

First Submitted

Initial submission to the registry

April 30, 2021

Completed
2 months until next milestone

First Posted

Study publicly available on registry

June 14, 2021

Completed
5.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2026

Last Updated

February 3, 2025

Status Verified

October 1, 2024

Enrollment Period

6.3 years

First QC Date

April 30, 2021

Last Update Submit

January 30, 2025

Conditions

Keywords

Cardiac DiseaseRegulatory T cellImmunomodulationTolerogenic protocolImmunosuppressive AgentsImmunologic Factors

Outcome Measures

Primary Outcomes (1)

  • Repopulation of Treg cells in the patient, determined as the increase of Treg values in peripheral blood with respect to pre-transplant values or in comparison with a control cohort of non-treated patients.

    24 months

Secondary Outcomes (8)

  • Incidence of episodes of acute myocardial rejection (diagnosed by echocardiography) that require treatment in the 2 years post-transplant

    24 months

  • Number of Treg cells in peripheral blood

    24 months

  • Change in the number of naive and memory Treg cells, and the production/levels of interferon gamma and interleukins (IL-4, IL-17A and IL-10).

    24 months

  • Decrease of cell subsets related with rejection (CD8 T cells subsets, activated T cells, antibody-secreting B cells) during the post-transplant follow-up period.

    24 months

  • Overall patient survival rate at 24 months.

    24 months

  • +3 more secondary outcomes

Study Arms (2)

10.000.000 thyTreg /kg

EXPERIMENTAL

Autologous thyTreg 10.000.000

Biological: Autologous thyTreg

20.000.000 thyTreg /kg

EXPERIMENTAL

Autologous thyTreg 20.000.000

Biological: Autologous thyTreg

Interventions

Treg lymphocytic cells, differentiated, autologous, of thymic tissue, expanded and stimulated with Interleukin (IL-) 2 (thyTreg)

Also known as: thyTreg cells
10.000.000 thyTreg /kg20.000.000 thyTreg /kg

Eligibility Criteria

AgeUp to 2 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17)

You may qualify if:

  • Patient under two years of age, who meets all the necessary requirements to undergo a heart transplant.
  • Patients without contraindication to immunosuppressive drugs.
  • Parents and/or guardians must be willing and able to understand the purpose and risks of the study and must sign the informed consent document

You may not qualify if:

  • Patients with DiGeorge Syndrome, since their thymic function is affected.
  • Human immunodeficiency virus positive serology
  • Epstein-Barr virus active infection
  • Patients hyperimmunized with cytotoxic anti-human leukocyte antigen antibodies
  • Patients with a history of previous malignancy
  • Patients who have participated in other intervention studies in the last month.
  • Patients who have received induction therapy with Basiliximab or Thymoglobulin.
  • Patients who have previously been thymectomized or transplanted.
  • Patients who have been diagnosed with severe autoimmune disease (celiac disease, autoimmune hypothyroidism, autoimmune diabetes)
  • Patients who will receive an asystole heart

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Hospital General Universitario Gregorio Marañon

Madrid, Madrid, 28007, Spain

RECRUITING

Related Publications (1)

  • Bernaldo-de-Quiros E, Cozar B, Lopez-Esteban R, Clemente M, Gil-Jaurena JM, Pardo C, Pita A, Perez-Caballero R, Camino M, Gil N, Fernandez-Santos ME, Suarez S, Pion M, Martinez-Bonet M, Correa-Rocha R. A Novel GMP Protocol to Produce High-Quality Treg Cells From the Pediatric Thymic Tissue to Be Employed as Cellular Therapy. Front Immunol. 2022 May 16;13:893576. doi: 10.3389/fimmu.2022.893576. eCollection 2022.

MeSH Terms

Conditions

Heart Diseases

Condition Hierarchy (Ancestors)

Cardiovascular Diseases

Study Officials

  • Rafael Correa-Rocha, PhD

    Hospital General Universitario Gregorio Marañon

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Rafael Correa-Rocha, PhD

CONTACT

Diana Hernandez Florez, PhD

CONTACT

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Group Leader

Study Record Dates

First Submitted

April 30, 2021

First Posted

June 14, 2021

Study Start

September 10, 2020

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

December 31, 2026

Last Updated

February 3, 2025

Record last verified: 2024-10

Data Sharing

IPD Sharing
Will not share

Locations