Transcriptome Sequencing Analysis of Whole Blood From Patients With Trigeminal Neuralgia

NCT04923399 | Unknown

• 1 other identifier: 2020PHB265-01
• Study Type: observational
• Target: 20
• Locations: 0 countries
• Sites: N/A

Brief Summary

Trigeminal neuralgia is a type of neuropathic pain that brings great physical and psychological pressure to patients. Chronic pain can cause changes in the composition of central and peripheral body fluids, and these changes may be useful for the prediction and treatment of pain. In this study, the whole blood of patients with trigeminal neuralgia and non-chronic pain was collected, and transcriptome sequencing (RNA-seq) was performed to determine the peripheral transcriptome changes of trigeminal neuralgia (TN) patients. And compare the expression of neuropeptide Y (NPY) in plasma and cerebrospinal fluid of TN and non-chronic pain patients.

Conditions

Trigeminal Neuralgia; Blood; Plasma; Cerebrospinal Fluid

Outcome Measures

Primary Outcomes (4)

• Visual Analogue Score (VAS)

  VAS score is a pain score

  The VAS score is tested before surgery in the preoperative preparation room

• Differentially expressed genes

  Transcriptome analysis of whole blood cell from patients with trigeminal neuralgia or non-chronic pain reveals differential gene expression between the two groups

  blood will be taken from two groups of patients before operation

• Differentially expressed genes (DEGs) enrichment pathway

  We will perform pathway enrichment analysis on the DEGs obtained by transcriptome analysis of the whole blood cell from patients with trigeminal neuralgia or non-chronic pain.

  blood will be taken from two groups of patients before operation

• Neuropeptide y expression

  We detect neuropeptide Y levels in the cerebrospinal fluid and plasma of patients with trigeminal neuralgia and non-chronic pain

  Cerebrospinal fluid will be collected during the surgery; Plasma will be collected before the surgery in the preoperative preparation room.

Study Arms (2)

TN (Trigeminal Neuralgia) group

Trigeminal neuralgia patients undergoing trigeminal nerve microvascular decompression

Non-TN group

patients without chronic pain or history of chronic pain

Eligibility Criteria

• Age: 20 Years - 75 Years
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Trigeminal neuralgia patients receive the trigeminal nerve microvascular decompression surgery. The comparsion group is patients without chronic pain or history of chronic pain. The whole blood cell, plasma and cerebrospinal fluid will be collected during the perioperative period.

You may qualify if:

• Patients with primary trigeminal neuralgia receive trigeminal nerve microvascular decompression, and healthy people without a history of chronic pain or patients with facial nerve palsy without chronic pain receive facial nerve decompression as a control;
• Age from 30 to 60 years old
• Complete white blood cell count: 4.8\~11.0x109/L

You may not qualify if:

• Patients with co-infection and tumor
• Patients with craniocerebral disease

Sponsors & Collaborators

• Peking University People's Hospital: lead

Related Publications (4)

• Montano N, Conforti G, Di Bonaventura R, Meglio M, Fernandez E, Papacci F. Advances in diagnosis and treatment of trigeminal neuralgia. Ther Clin Risk Manag. 2015 Feb 24;11:289-99. doi: 10.2147/TCRM.S37592. eCollection 2015.

  PMID: 25750533 BACKGROUND

• Song N, Liao DQ, Liu F, Zhang YY, Lin J, Wang H, Shen JF. Effect of Titanium Particles on the Voltage-Gated Potassium Channel Currents in Trigeminal Root Ganglion Neurons. Implant Dent. 2019 Feb;28(1):54-61. doi: 10.1097/ID.0000000000000848.

  PMID: 30507652 BACKGROUND

• Eroli F, Loonen ICM, van den Maagdenberg AMJM, Tolner EA, Nistri A. Differential neuromodulatory role of endocannabinoids in the rodent trigeminal sensory ganglion and cerebral cortex relevant to pain processing. Neuropharmacology. 2018 Mar 15;131:39-50. doi: 10.1016/j.neuropharm.2017.12.013. Epub 2017 Dec 7.

  PMID: 29225040 BACKGROUND

• Liu T, Xu C, Guo J, He Z, Zhang Y, Feng Y. Whole Blood Transcriptome Analysis in Patients with Trigeminal Neuralgia: a Prospective Clinical Study. J Mol Neurosci. 2024 Feb 1;74(1):16. doi: 10.1007/s12031-024-02195-6.

  PMID: 38300339 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

whole blood cell; plasma; cerebrospinal fluid

MeSH Terms

Conditions

Trigeminal Neuralgia

Condition Hierarchy (Ancestors)

Trigeminal Nerve Diseases; Facial Neuralgia; Facial Nerve Diseases; Mouth Diseases; Stomatognathic Diseases; Cranial Nerve Diseases; Nervous System Diseases

Study Officials

• Yi Feng, MD, PhD

  Peking University People's Hospital

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Yi Feng, MD,PhD

CONTACT

+861088325581; yifeng65@sina.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Target Duration: 3 Months
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Chief of the Anesthesiology Department

Study Record Dates

• First Submitted: June 6, 2021
• First Posted: June 11, 2021
• Study Start: June 1, 2021
• Primary Completion: June 1, 2023
• Study Completion: June 1, 2023
• Last Updated: June 11, 2021

Record last verified: 2021-06