NCT04883164

Brief Summary

The purpose of the research is to evaluate new blood tests, which measure immunity to the COVID-19 coronavirus after vaccination. These tests will be used to measure T-cell and antibody immunity after COVID-19 vaccination. Recent studies show that less than one-fifth of chronically immunosuppressed transplant recipients developed anti-receptor-binding domain antibodies after the first dose of the Pfizer vaccine (Boyarski, 2021). ood sampling at periodic intervals. These samples will be used to measure T-cell and antibody immunity to the COVID-19 coronavirus.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
300

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Apr 2021

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

April 27, 2021

Completed
14 days until next milestone

First Submitted

Initial submission to the registry

May 11, 2021

Completed
1 day until next milestone

First Posted

Study publicly available on registry

May 12, 2021

Completed
1.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 31, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 31, 2023

Completed
Last Updated

May 12, 2021

Status Verified

May 1, 2021

Enrollment Period

1.9 years

First QC Date

May 11, 2021

Last Update Submit

May 11, 2021

Conditions

Outcome Measures

Primary Outcomes (2)

  • T-cells and other immune cells reactive to the spike antigenic protein and its components

    as above, will be measured with flow cytometry

    2 years

  • Binding and neutralizing antibodies to the spike protein and its earlier compoent

    Antibodies will be measured by ELISA

    2 years

Secondary Outcomes (1)

  • COVID-19 infection

    2 years

Study Arms (2)

Healthy non-immunocompromized subjects

Healthy individuals are those with no pre-existing conditions that cause immune deficiency, and who are not receiving drugs to suppress the immune system. •

Other: Cellular and antibody response to spike antigens of SARS-CoV-2 in both groups in peripheral blood samples

Immunocompromized

Immunocompromised subjects are those receiving immunosuppressive or immunomodulatory drugs such as those given for autoimmune disease, inflammatory bowel disease, malignancies and transplantation. Bone marrow transplant recipients and subjects with known immune deficiency diseases are also considered immunocompromised.

Other: Cellular and antibody response to spike antigens of SARS-CoV-2 in both groups in peripheral blood samples

Interventions

Cellular immunity will be assessed with T-cells and other immune cells that express CD154 or other inflammatory or other markers after stimulation with spike antigens. Antibody immunity will be measured with binding and neutralizing activity of antibodies to spike antigens.

Healthy non-immunocompromized subjectsImmunocompromized

Eligibility Criteria

Age18 Years - 100 Years
Sexall
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

Subjects age 18 years or older, male or female, • anyone considering COVID-19 vaccination or anyone that has received COVID-19 vaccination. • Subjects can enroll at anytime after vaccination even though they may not have enrolled before vaccination. * For individuals previously tested at Plexision for other purposes, and who have since been vaccinated, residual cells stored for quality control and potential repeat testing will be used to establish earlier measurement of cellular and antibody immunity. * Medication history such as immunosuppressive or immune modulatory drugs that are being used, * Underlying conditions related to immunosuppressive or immunomodulatory drug use, type of COVID-19 vaccine and doses received at the time of enrollment. * Other underlying conditions such as diabetes, hypertension and asthma.

You may qualify if:

  • IRB-approved informed consent,
  • age 18 years or older, male or female,
  • anyone considering COVID-19 vaccination or anyone that has received COVID-19 vaccination.
  • Subjects can enroll at anytime after vaccination even though they may not have enrolled before vaccination.
  • For individuals previously tested at Plexision for other purposes, and who have since been vaccinated, residual cells stored for quality control and potential repeat testing will be used to establish earlier measurement of cellular and antibody immunity as described in Table 1.

You may not qualify if:

  • Failure to provide informed consent

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Plexision

Pittsburgh, Pennsylvania, 15224, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITHOUT DNA

Whole blood samples, White blood cells extracted from whole blood samples, serum and plasma

MeSH Terms

Interventions

Cell Count

Intervention Hierarchy (Ancestors)

Cytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisInvestigative TechniquesCell Physiological Phenomena

Study Officials

  • Ashok Reddy, BE

    Plexision

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Ashok Reddy, BE

CONTACT

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 11, 2021

First Posted

May 12, 2021

Study Start

April 27, 2021

Primary Completion

March 31, 2023

Study Completion

March 31, 2023

Last Updated

May 12, 2021

Record last verified: 2021-05

Data Sharing

IPD Sharing
Will not share

Plexision offers antibody testing of IgG to RBD and spike proteins as clinical tests to look for infection status. Because these tests are being used to perform antibody immunity testing after vaccination in this study, Plexision will inform participants about results of this testing as they become available.

Locations