NCT04847349

Brief Summary

This pilot double-blind randomized controlled trial will test the preliminary efficacy of two doses of a combination of live microbials (probiotics) given to boost the immunity of unvaccinated persons previously infected with SARS-CoV-2.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
54

participants targeted

Target at P25-P50 for not_applicable covid19

Timeline
Completed

Started Apr 2021

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

April 13, 2021

Completed
Same day until next milestone

Study Start

First participant enrolled

April 13, 2021

Completed
6 days until next milestone

First Posted

Study publicly available on registry

April 19, 2021

Completed
7 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2021

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

January 31, 2022

Completed
Last Updated

February 1, 2022

Status Verified

January 1, 2022

Enrollment Period

7 months

First QC Date

April 13, 2021

Last Update Submit

January 31, 2022

Conditions

Keywords

Probiotics

Outcome Measures

Primary Outcomes (1)

  • Change in titer of serum anti-SARS-CoV-2 immunoglobulin G (IgG)

    between baseline and day 21 (day 1 is the first day of taking the investigational product)

Secondary Outcomes (8)

  • Change in the titer of serum anti-SARS-CoV-2 IgG

    between baseline and day 42

  • Change in the titer of serum anti-SARS-CoV-2 neutralizing antibody

    between baseline and days 21 and 42

  • Change in the titer of serum and nasal anti-SARS-CoV-2 immunoglobulin A (IgA) antibody

    between baseline and days 21 and 42

  • In-vitro changes in cytokine release in response to stimulation of peripheral blood mononuclear cells with SARS-CoV-2 peptides

    between baseline and days 21 and 42

  • Serum cytokine levels

    between baseline and days 21 and 42

  • +3 more secondary outcomes

Study Arms (3)

Live microbial (Probiotic) consortium OL-1, standard dose

EXPERIMENTAL

Combination of a standard dose of live microbials (probiotics) taken as a capsule once per day with breakfast for 21 days. Capsules should not be consumed with hot drinks or alcohol.

Dietary Supplement: OL-1, standard dose

Live microbial (Probiotic) consortium OL-1, high dose

EXPERIMENTAL

Combination of a high dose of live microbials (probiotics) taken as a capsule once per day with breakfast for 21 days. Capsules should not be consumed with hot drinks or alcohol.

Dietary Supplement: OL-1, high dose

Placebo for live microbial (probiotic) consortium

PLACEBO COMPARATOR

Capsule containing inactive ingredients such as a product of potato starch (maltodextrin), but no probiotics, taken once per day with breakfast for 21 days.

Dietary Supplement: Placebo

Interventions

OL-1, standard doseDIETARY_SUPPLEMENT

The live microbial components have been present in the food supply, have a long history of safe use in foods, and are already marketed in the United States.

Live microbial (Probiotic) consortium OL-1, standard dose
OL-1, high doseDIETARY_SUPPLEMENT

The live microbial components have been present in the food supply, have a long history of safe use in foods, and are already marketed in the United States.

Live microbial (Probiotic) consortium OL-1, high dose
PlaceboDIETARY_SUPPLEMENT

Capsule containing inactive ingredients such as a product of potato starch (maltodextrin), but no probiotics.

Placebo for live microbial (probiotic) consortium

Eligibility Criteria

Age18 Years - 60 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy adults (any gender, ages 18 to 60 years old) in good general health, based on medical history
  • SARS-CoV-2 infection \>4 months prior confirmed by a positive polymerase chain reaction (PCR) or antigen test
  • Body mass index (BMI) of 18.5-39.9 kg/m2
  • Agreement to comply with the protocol and study restrictions
  • Access to internet in addition to willingness and ability to use web-based questionnaires
  • Available for all study visits
  • English-speaking

You may not qualify if:

  • Recent infection with coronavirus disease-2019 (COVID-19) (newly positive PCR or antigen test within prior 4 months)
  • Prior receipt of antibody therapies (convalescent or monoclonal) towards COVID-19
  • Prior receipt of vaccines against COVID-19
  • Prior or current participation in a clinical trial of vaccines against COVID-19
  • Scheduled to receive a vaccine against COVID-19 in the following month
  • Regular use of any live microbial (probiotic) supplements
  • Any acute or chronic respiratory tract disease besides mild to moderate asthma
  • Any diagnosed immunodeficiency
  • Current use of immunosuppressive drugs
  • Any diagnosis of chronic gut disease, such as inflammatory bowel disease or irritable bowel syndrome
  • Pregnancy or breastfeeding
  • Recent use of antibiotics
  • Current addiction to alcohol, drugs, or medications

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Rutgers University

New Brunswick, New Jersey, 08901, United States

Location

Related Publications (1)

  • Horton DB, Ukey R, Madhvi A, Andrews T, Parmar V, Reilly N, Makela SM, Peterson J, Hustad L, Wong G, Barrett ES, Bruiners N, Carson JL, Getz K, Greenberg P, Iizuka A, Roy J, Pastuszak AW, Lehtinen MJ, Blaser MJ, Panettieri RA Jr, Gennaro ML. Live microbials to boost Anti-SARS-CoV-2 immunity clinical trial (Live BASIC trial): a triple-blind randomized controlled trial. Infection. 2025 Nov 26. doi: 10.1007/s15010-025-02697-4. Online ahead of print.

MeSH Terms

Conditions

COVID-19

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Study Officials

  • Daniel B Horton, MD, MSCE

    Rutgers University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant Professor of Pediatrics and Epidemiology

Study Record Dates

First Submitted

April 13, 2021

First Posted

April 19, 2021

Study Start

April 13, 2021

Primary Completion

November 1, 2021

Study Completion

January 31, 2022

Last Updated

February 1, 2022

Record last verified: 2022-01

Data Sharing

IPD Sharing
Will share

Saliva, nasal rinse, blood samples, and stool samples collected will be used for the purposes of this research. After information that could identify consenting participants has been removed, de-identified information and left over biospecimens collected for this research may be used by or distributed to the study funder (Danisco USA Inc.) or to investigators for other research without obtaining additional informed consent. Personal identifying details, such as name and date of birth, will not be shared without participants' additional permission.

Shared Documents
STUDY PROTOCOL, SAP, ICF, CSR
Time Frame
Data will be shared after enrollment and data analysis are completed.

Locations