NCT04817917

Brief Summary

This study is to evaluate the seroprevalence of neutralizing antibodies against Japanese encephalitis (JE) virus in children aged 6 years who were previously administered with 5 different immunization strategies by JE attenuated live vaccine (JEV-L) or/and inactivated vaccine (JEV-I). The secondary objective is to evaluate the immunogenicity of the booster dose of JEV-I at 6 years old for those previously immunized with 3 doses of JEV-I or those sequential administered with 1 dose of JEV-L and another dose of JEV-I.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
712

participants targeted

Target at P75+ for phase_4

Timeline
Completed

Started Mar 2021

Typical duration for phase_4

Geographic Reach
1 country

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 1, 2021

Completed
22 days until next milestone

First Submitted

Initial submission to the registry

March 23, 2021

Completed
3 days until next milestone

First Posted

Study publicly available on registry

March 26, 2021

Completed
1.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 17, 2022

Completed
10 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2023

Completed
Last Updated

November 15, 2022

Status Verified

July 1, 2022

Enrollment Period

1.5 years

First QC Date

March 23, 2021

Last Update Submit

November 13, 2022

Conditions

Keywords

Japanese EncephalitisSeroprevalenceImmunogenicityImmunization scheduleSafety

Outcome Measures

Primary Outcomes (2)

  • Proportions of seropositivity of neutralizing antibodies against JE at the age of 6

    Defined as any positive antibody (neutralizing antibody titers ≥1:10) response in children aged 6.

    At the age of 6. Test immediately after enrollment by a dose of blood sample.

  • Geometric Mean Titers (GMTs) or Median antibody titers for children aged 6 years

    Neutralizing antibody titers by plaque-reduction neutralization tests (PRNT).

    At the age of 6. Test immediately after enrollment by a dose of blood sample.

Secondary Outcomes (4)

  • Seroconversion rates of the booster dose of JEV-I for Group 1 and Group 2

    before and 28-35 days after the booster dose.

  • Proportion of seropositivity of neutralizing antibodies against JE after the booster dose for Group 1 and Group 2.

    28-35 days after the booster dose.

  • Geometric Mean Titers (GMTs) or Median antibody titers after the booster dose for Group 1 and Group 2.

    28-35 days after the booster dose.

  • Safety of the booster dose for Group 1 and Group 2

    From inoculation to 30 days after the inoculation.

Study Arms (5)

3JEV-I (Group 1)

EXPERIMENTAL

Retrospective: 3 doses of JE vaccines. 2 doses of Inactivated JE Vaccine (JEV-I, 7-10 days apart) at 8 months of age and 1 dose of JEV-I at 2 years old. Prospective: 1 booster dose of JEV-I at 6 years old.

Biological: Japanese Encephalitis Vaccine (Vero Cell), Inactivated;

JEV-L+JEV-I (Group 2)

EXPERIMENTAL

Retrospective: 2 doses of JE vaccines. 1 dose of JE attenuated live vaccine (JEV-L) at 8 months of age and 1 dose of JEV-I at 2 years old. Prospective: 1 booster dose of JEV-I at 6 years old.

Biological: Japanese Encephalitis Vaccine (Vero Cell), Inactivated;

JEV-L+2JEV-I (Group 3)

EXPERIMENTAL

Retrospective: 3 doses of JE vaccines. 1 dose of JEV-L at 8 months of age and 2 dose (7-10 days apart) of JEV-I at 2 years old.

Other: No prospective interventions

2JEV-I+JEV-L (Group 4)

EXPERIMENTAL

Retrospective: 3 doses of JE vaccines. 2 doses of JEV-I (7-10 days apart) at 8 months of age and 1 dose of JEV-L at 2 years old.

Other: No prospective interventions

2JEV-L (Group 5)

EXPERIMENTAL

Retrospective: 2 doses of JE vaccines. 2 doses of JEV-L respectively administered at 8 months of age and 2 years old.

Other: No prospective interventions

Interventions

The investigators recruit 6 years old children who have been vaccinated by 2-3 doses of JE vaccines with different immunization strategies. For participants from Group 1 and Group 2 who haven't completed the JE immunization schedules, a booster dose of JEV-I will be administered at 6 years old.

3JEV-I (Group 1)JEV-L+JEV-I (Group 2)

The investigators recruit 6 years old children who have been vaccinated by 2-3 doses of JE vaccines with different immunization strategies. Participants from Group 3-5 have completed the JE immunization schedules with JEV-I or/and JEV-L. No prospective intervention will be given.

2JEV-I+JEV-L (Group 4)2JEV-L (Group 5)JEV-L+2JEV-I (Group 3)

Eligibility Criteria

Age72 Months - 75 Months
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17)

You may qualify if:

  • Participants aged ≥ 72 months to \< 75 months.
  • Participants have completed routine immunization schedules in time.
  • Participants are healthy native residents.
  • Parents/guardians can understand the protocol requirements, provide informed consent, accept all scheduled visits, and comply with all trial procedures.

You may not qualify if:

  • History of neurological diseases or related symptoms.
  • An acute illness with fever (temperature ≥ 37.1℃) or any acute onset of chronic diseases.
  • Known impairment of immunologic function, or receipt of immunosuppressive therapy or immunoglobulin in the past 3 months.
  • Known allergy to any constituent of the vaccine.
  • Have been injected with other vaccines or any clinical trial drugs in the past 4 weeks.
  • Any condition which, in the opinion of the investigator, would pose a health risk to the subject or interfere with the vaccine.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Liandu center for disease control and prevention

Lishui, Zhejiang, 323000, China

Location

Kecheng center for disease control and prevention

Quzhou, Zhejiang, 324000, China

Location

Pingyang center for disease control and prevention

Wenzhou, Zhejiang, 325400, China

Location

MeSH Terms

Conditions

Encephalitis, JapaneseVaccine-Preventable Diseases

Interventions

Japanese Encephalitis Vaccines

Condition Hierarchy (Ancestors)

Encephalitis, ArbovirusEncephalitis, ViralCentral Nervous System Viral DiseasesCentral Nervous System InfectionsInfectionsInfectious EncephalitisArbovirus InfectionsVector Borne DiseasesMosquito-Borne DiseasesVirus DiseasesRNA Virus InfectionsFlavivirus InfectionsFlaviviridae InfectionsEncephalitisBrain DiseasesCentral Nervous System DiseasesNervous System DiseasesNeuroinflammatory Diseases

Intervention Hierarchy (Ancestors)

Viral VaccinesVaccinesBiological ProductsComplex Mixtures

Study Design

Study Type
interventional
Phase
phase 4
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER GOV
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 23, 2021

First Posted

March 26, 2021

Study Start

March 1, 2021

Primary Completion

September 17, 2022

Study Completion

June 30, 2023

Last Updated

November 15, 2022

Record last verified: 2022-07

Locations