Protein Kinase 2 (HIPK2) Polymorphisms on rs2058265, rs6464214, and rs7456421

NCT04804436 | Completed

• 1 other identifier: Geneticstone
• Study Type: interventional
• Target: 196
• Locations: 1 country
• Sites: 1

Brief Summary

In the present study investigators aimed to investigate whether homeodomain interacting protein kinase 2 (HIPK2) polymorphism is associated with renal stone formation in Turkish population or not. One hundred and twenty nine participants with calcium nephrolithiasis and 67 sex and age-matched healthy controls were enrolled in this study. For analysis of HIPK2 polymorphism, the real-time PCR amplification was performed in a final volume of 20μL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination. Chi square test was utilized to compare the differences of the genotype and allele frequencies between patients and controls.

Conditions

Kidney Stone

Keywords

KSD HIPK2 gene Polymorphism

Outcome Measures

Primary Outcomes (1)

• Single nucleotide polymorphism

  Single nucleotide polymorphism incidence on rs2058265, rs6464214, and rs7456421

  One year

Study Arms (2)

Patients with nephrolithiasis

EXPERIMENTAL

The real-time PCR amplification was performed in a final volume of 20μL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. Thermal cycling conditions were as follows: initial denaturation at 94℃ for 3 min, 40 cycles of 94℃ for 15 s, and 60°C for 1 min. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination.

Genetic: Genetic analysis

Healthy control group

EXPERIMENTAL

he real-time PCR amplification was performed in a final volume of 20μL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. Thermal cycling conditions were as follows: initial denaturation at 94℃ for 3 min, 40 cycles of 94℃ for 15 s, and 60°C for 1 min. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination.

Genetic: Genetic analysis

Interventions

Genetic analysis GENETIC

he real-time PCR amplification was performed in a final volume of 20μL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. Thermal cycling conditions were as follows: initial denaturation at 94℃ for 3 min, 40 cycles of 94℃ for 15 s, and 60°C for 1 min. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination.

Healthy control group; Patients with nephrolithiasis

Eligibility Criteria

• Age: 38 Years - 68 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Patients with nephrolithiasis

You may not qualify if:

• Patients had a history of chronic urinary tract infection
• renal failure
• gastrointestinal diseases
• increased levels of vitamin D
• sarcoidosis
• primary hyperoxaluria
• polycystic kidney disease, gout, renal tubular acidosis, primary and secondary hyperparathyroidism.

Sponsors & Collaborators

• Saglik Bilimleri Universitesi: lead

Study Sites (1)

Omer Gokhan Doluoglu

Ankara, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Kidney Calculi

Interventions

Genetic Testing

Condition Hierarchy (Ancestors)

Nephrolithiasis; Kidney Diseases; Urologic Diseases; Female Urogenital Diseases; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Urolithiasis; Urinary Calculi; Male Urogenital Diseases; Calculi; Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Investigative Techniques; Genetic Techniques; Genetic Services; Health Services; Health Care Facilities Workforce and Services; Diagnostic Services; Preventive Health Services

Study Officials

• Cavit Ceylan, Professor

  University of Medical Sciences, Ministry of Health, Ankara City Hospital, Department of Urology, Ankara, Turkey

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: SCREENING
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: March 14, 2021
• First Posted: March 18, 2021
• Study Start: August 1, 2018
• Primary Completion: October 6, 2019
• Study Completion: October 6, 2019
• Last Updated: March 18, 2021

Record last verified: 2021-03

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)