Association of Autophagy-related Genes ,LncRNA and SNPs With Colorectal Cancer in Egyptian Population

NCT04729855 | Completed

• 1 other identifier: Esraa Hassan MD Protocol
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

1. 1.Determination of expression level of HOTTIP and EIF4EBP1(Eukaryotic translation initiation factor 4E-binding protein 1) .
2. 2.Investigation of the SNP HOTTIP rs1859168 and it's association with CRC susceptibility.
3. 3.Correlation of the expression of these genes with various stages of CRC to determine the prognostic value of each of them.

Conditions

Colo-rectal Cancer

Keywords

Colorectal cancer, HOTTIP

Outcome Measures

Primary Outcomes (1)

• HOTTIP gene expression

  Estimation of expression level of HOTTIP in peripheral blood mononuclear cells and tissue aiming to use as possible less invasive prognostic markers for colorectal cancer.

  2 years

Secondary Outcomes (1)

• EIF4EBP

  2 years

Study Arms (2)

colorectal cancer patients

Colorectal cancer patients( n = 30 ) from stage I-III with the following criteria:- a. Inclusion criteria: * No sex predilection. * Age (25-65 yr). * Colorectal carcinoma confirmed by histopathological examination

Diagnostic Test: PBMCs and Tissue expression of HOTTIP ,EIF4EBP1 and serum SNP HOTTIP rs1859168

Healthy controls ( n = 30)

matched as regarding age and sex as much as possible with cases.

Diagnostic Test: PBMCs and Tissue expression of HOTTIP ,EIF4EBP1 and serum SNP HOTTIP rs1859168

Interventions

PBMCs and Tissue expression of HOTTIP ,EIF4EBP1 and serum SNP HOTTIP rs1859168 DIAGNOSTIC_TEST

5 ml fresh blood sample, normal and CRC tissue

Healthy controls ( n = 30); colorectal cancer patients

Eligibility Criteria

• Age: 25 Years - 65 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Healthy controls ( n = 30) matched as regarding age and sex as much as possible with cases. -Colorectal cancer patients( n = 30 ) from stage I-III with the following criteria:-

You may qualify if:

• No sex predilection.
• Age (25-65 yr).
• Colorectal carcinoma confirmed by histopathological examination

You may not qualify if:

• patients who received any treatment.
• Cases with other organ cancers.
• History of chemotherapy and radiotherapy.
• Systemic diseases such as renal failure, diabetes mellitus, and hypertension.

Sponsors & Collaborators

• Esraa Hassan,MD: lead
• South Egypt Cancer Institute: collaborator

Study Sites (1)

Assiut University

Asyut, Egypt

Location

Related Publications (14)

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• Hu P, Qiao O, Wang J, Li J, Jin H, Li Z, Jin Y. rs1859168 A > C polymorphism regulates HOTTIP expression and reduces risk of pancreatic cancer in a Chinese population. World J Surg Oncol. 2017 Aug 17;15(1):155. doi: 10.1186/s12957-017-1218-0.

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• Shang A, Wang W, Gu C, Chen C, Zeng B, Yang Y, Ji P, Sun J, Wu J, Lu W, Sun Z, Li D. Correction to: Long non-coding RNA HOTTIP enhances IL-6 expression to potentiate immune escape of ovarian cancer cells by upregulating the expression of PD-L1 in neutrophils. J Exp Clin Cancer Res. 2020 Dec 4;39(1):272. doi: 10.1186/s13046-020-01755-z.

  PMID: 33272287 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

EDTA (ethylene diamine tetra acetic acid)coated fresh blood sample

MeSH Terms

Conditions

Colonic Neoplasms; Colorectal Neoplasms

Condition Hierarchy (Ancestors)

Intestinal Neoplasms; Gastrointestinal Neoplasms; Digestive System Neoplasms; Neoplasms by Site; Neoplasms; Digestive System Diseases; Gastrointestinal Diseases; Colonic Diseases; Intestinal Diseases; Rectal Diseases

Study Officials

• Esraa H Mohamed, Master

  Assistant lecturer of Medical Microbiology and Immunology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principle investigator

Study Record Dates

• First Submitted: January 24, 2021
• First Posted: January 29, 2021
• Study Start: April 15, 2022
• Primary Completion: May 20, 2023
• Study Completion: September 20, 2024
• Last Updated: April 30, 2025

Record last verified: 2025-04

Locations

EG (1)