NCT04716907

Brief Summary

The main clinical manifestation associated with SARS-CoV-2 infection is an influenza-like illness that follows the infection of the respiratory tract. In a few percent of infected people, inflammation of the lungs leads to severe pneumonia that requires hospitalization, in intensive care units for the more severe cases. Despite intensive care, a fatal outcome occurs in 6% and 12% of women and men over 80 years of age hospitalized for severe COVID, respectively. Factors associated with a higher risk of death in patients with SARS-CoV-2 include age and low circulating lymphocyte counts. Significant lymphopenia is indeed frequently observed in patients with severe COVID-19 and both phenotypic and functional changes in antiviral T cells have been correlated with the severity of COVID-19. The thymus, the organ that produces T lymphocytes, undergoes progressive physiological involution with age. However, in the elderly, rare cases of thymic hyperplasia are reported in autoimmune diseases or cancers, or are observed in response to deep lymphopenia, whether or not associated with sepsis. This cohort of patients treated for a SARS-CoV-2 infection could allow to better understand the role of the thymus in this pathology.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
85

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Mar 2021

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

January 16, 2021

Completed
4 days until next milestone

First Posted

Study publicly available on registry

January 20, 2021

Completed
2 months until next milestone

Study Start

First participant enrolled

March 19, 2021

Completed
1 year until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 2, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 2, 2022

Completed
Last Updated

April 8, 2026

Status Verified

April 1, 2026

Enrollment Period

1 year

First QC Date

January 16, 2021

Last Update Submit

April 2, 2026

Conditions

Keywords

Covid19Thymic functionGenetic polymorphism

Outcome Measures

Primary Outcomes (1)

  • Genetic Predisposition to severe forms of COVID-19

    Test of association between single-nucleotide polymorphisms (SNP) within the TCRA/D region known to influence the level of thymopoiesis (Clave et al., Sci Transl Med., 2018) and CT scan classification of COVID-associated pneumopathy (0=Absent or minor pulmonary parenchymal changes ; 1=Limited ground-glass opacities ; 2=Bilateral ground-glass opacities \< 50% of pulmonary parenchyma ; 3=Idem 2, with superimposed inter/intra lobular septal thickening, i.e. 'crazy paving' ; 4=Bilateral ground-glass opacities \> 50% of pulmonary parenchyma ; 5=Idem 4, with superimposed 'crazy paving' ; 6=Idem 5, with pulmonary fibrosis).

    through study completion, average 1 year

Secondary Outcomes (8)

  • Genetic Predisposition to thymic enlargement observed during COVID-19 infection

    through study completion, average 1 year

  • Genetic Predisposition to enhanced thymic function during COVID-19 infection

    through study completion, average 1 year

  • Genetic Predisposition to severity of COVID-19 pathology

    through study completion, average 1 year

  • Basal thymic function in COVID patients

    through study completion, average 1 year

  • Thymic function in COVID patients

    through study completion, average 1 year

  • +3 more secondary outcomes

Study Arms (2)

Case : COVID-19 positive patients

patients hospitalized for COVID-19 infection

Genetic: Single-Nucleotide Polymorphisms (SNP) within the TCRA/D regionBiological: Blood sampleDiagnostic Test: CT ScanBiological: Bronchial fibroscopy

Control : COVID-19 negative patients

patients hospitalized for other reasons

Biological: Blood sampleDiagnostic Test: CT Scan

Interventions

Blood sampleBIOLOGICAL

Dosage of sj/βTREC ratio, lymphocytes, cytokines and chemokines.

Case : COVID-19 positive patientsControl : COVID-19 negative patients
CT ScanDIAGNOSTIC_TEST

Thymus and lung imaging

Case : COVID-19 positive patientsControl : COVID-19 negative patients

Bronchoalveolar lavage in mechanically ventilated patients for dosage of recent thymic emigrants in lungs

Case : COVID-19 positive patients

DNA extraction from blood samples, PCR and Sequencing

Case : COVID-19 positive patients

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

A total of 50 cases and 50 control subjects

You may qualify if:

  • Cases :
  • Patients with confirmed COVID-19 infection
  • Hospitalized for COVID-19 infection
  • Having signed a written informed consent form
  • Affiliation to the social security system
  • Controls :
  • Non-COVID-19 patients
  • Hospitalized for other reasons
  • Age and sex-matched controls
  • Having signed a written informed consent form,
  • Affiliation to the social security system

You may not qualify if:

  • Autoimmune disease
  • HIV, Hepatitis B or Hepatitis C
  • Pregnant or breastfeeding women
  • A mental or linguistic inability to understand the study
  • Patient under protection of the adults (guardianship, curators or safeguard of justice)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

CMC Ambroise Paré

Neuilly-sur-Seine, 92200, France

Location

Related Publications (1)

  • Roux HM, Marouf A, Dutrieux J, Charmeteau-De Muylder B, Figueiredo-Morgado S, Avettand-Fenoel V, Cuvelier P, Naudin C, Bouaziz F, Geri G, Couedel-Courteille A, Squara P, Marullo S, Cheynier R. Genetically determined thymic function affects strength and duration of immune response in COVID patients with pneumonia. Sci Adv. 2023 Sep 22;9(38):eadh7969. doi: 10.1126/sciadv.adh7969. Epub 2023 Sep 22.

MeSH Terms

Conditions

COVID-19

Interventions

Polymorphism, Single NucleotideBlood Specimen CollectionTomography, X-Ray Computed

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Polymorphism, GeneticGenetic VariationGenetic PhenomenaSpecimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative TechniquesImage Interpretation, Computer-AssistedDiagnostic ImagingRadiographic Image EnhancementImage EnhancementPhotographyRadiographyTomography, X-RayTomography

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 16, 2021

First Posted

January 20, 2021

Study Start

March 19, 2021

Primary Completion

April 2, 2022

Study Completion

April 2, 2022

Last Updated

April 8, 2026

Record last verified: 2026-04

Data Sharing

IPD Sharing
Will not share

Locations