The Effects of Antibiotic Combinations on Stem Cells
1 other identifier
observational
3
1 country
1
Brief Summary
The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for all trials
Started Jan 2014
Typical duration for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
January 1, 2014
CompletedPrimary Completion
Last participant's last visit for primary outcome
January 1, 2016
CompletedStudy Completion
Last participant's last visit for all outcomes
February 1, 2017
CompletedFirst Submitted
Initial submission to the registry
January 11, 2021
CompletedFirst Posted
Study publicly available on registry
January 19, 2021
CompletedJanuary 19, 2021
January 1, 2021
2 years
January 11, 2021
January 13, 2021
Conditions
Outcome Measures
Primary Outcomes (2)
Measurment of Cell Viability and Apoptosis
To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol.
1 year
Measurment of cell differantiation
To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions.
1 year
Study Arms (3)
Triple antibiotic paste(TAP)
ciprofloxacin, metronidazole and minocycline
Doble antibiotic paste(DAP)
ciprofloxacin and metronidazole
Modified triple antibiotic paste(mTAP)
ciprofloxacin, metronidazole and cefaclor
Interventions
Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
Eligibility Criteria
Tissue samples were obtained from the apex of the extracted third molar teeth of young adult patients aged between 16-22 .
You may qualify if:
- Patients with open apex third molar teeth for extraction indication
You may not qualify if:
- Patients with mature apex third molar teeth for extraction indication
Contact the study team to confirm eligibility.
Sponsors & Collaborators
- Eskisehir Osmangazi Universitylead
- TC Erciyes Universitycollaborator
Study Sites (1)
Eskişehir Osmangazi University
Eskişehir, 26040, Turkey (Türkiye)
Biospecimen
Tissue samples were obtained from the apex of the extracted third molar teeth of young adult patients aged between 16-22 (N=7).
MeSH Terms
Interventions
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Seçil Çalışkan
Eskişehir Osmangazi University
Study Design
- Study Type
- observational
- Observational Model
- CASE CONTROL
- Time Perspective
- PROSPECTIVE
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Assistant Professor
Study Record Dates
First Submitted
January 11, 2021
First Posted
January 19, 2021
Study Start
January 1, 2014
Primary Completion
January 1, 2016
Study Completion
February 1, 2017
Last Updated
January 19, 2021
Record last verified: 2021-01