Virological and Immunological Determinants of Arbovirus Infection in New Caledonia

NCT04619823 | Recruiting

• 1 other identifier: 2019-062
• Study Type: interventional
• Target: 250
• Locations: 1 country
• Sites: 1

Brief Summary

Arboviruses, diseases transmitted to humans by the bite of an insect vector, are a major public health problem, particularly in tropical and sub-tropical countries. In New Caledonia, dengue epidemics are recurrent and may be associated with the co-circulation of other arboviruses such as Zika or chikungunya. The virological determinants which condition the occurrence of these epidemics may be linked to an increased vectorial competence of the vector mosquito Aedes aegypti for a particular viral isolate. In fact, the Aedes aegypti mosquito is infected by making a blood meal on a person infected with an arbovirus. The virus infects its digestive tract, then spreads throughout the mosquito's body until it reaches its salivary glands. The virus is then present in the saliva and will be injected into the human host during a new blood meal. Some viral variants are best transmitted by Aedes aegypti. In general, the study of this vectorial competence is carried out by experiments in the laboratory during which an artificial blood meal composed of mammalian blood (human, rabbit, etc.) is mixed with a viral stock. Carrying out deported blood meals during which blood collected from patients infected with an arbovirus is used to gorge mosquitoes makes it possible to place oneself in experimental conditions as close as possible to the natural cycle of transmission of arboviruses. In the human host, cells of the myeloid lineage present in the peripheral blood constitute preferred targets of replication for arboviruses. At the same time, the peripheral blood cells of patients are activated in response to infection and secrete many soluble factors released into the blood of patients. The study of blood samples from patients infected with arboviruses is therefore of prime importance for understanding both the replicative mechanisms of arboviruses but also the immune response they induce.

Conditions

Arbovirus Infections; Zika; Dengue; Chikungunya

Keywords

New Caledonia

Outcome Measures

Primary Outcomes (3)

• Measurement of vector competence parameters of Aedes aegypti (carriers or not of Wolbachia) for arboviruses by vector competence experiments using deported blood meals.

  Rate of infection of Aedes aegypti (carriers or not of Wolbachia) for arboviruses

  5 years

• Measurement of vector competence parameters of Aedes aegypti (carriers or not of Wolbachia) for arboviruses by vector competence experiments using deported blood meals.

  Measurement of dissemination of Aedes aegypti (carriers or not of Wolbachia) for arboviruses

  5 years

• Measurement of vector competence parameters of Aedes aegypti (carriers or not of Wolbachia) for arboviruses by vector competence experiments using deported blood meals.

  Measurement of transmission of Aedes aegypti (carriers or not of Wolbachia) for arboviruses

  5 years

Secondary Outcomes (2)

• Molecular characterization of arborviruses strains included in the blood of patients and which may be diffused by mosquitoes carrying Wolbachia or not during deported blood meals

  5 years

• Molecular characterization of arborviruses strains contained in mosquitoes present in patients' homes

  5 years

Study Arms (1)

Patient infected by arboviruses

OTHER

Other: Blood sample collection

Interventions

Blood sample collection OTHER

Blood sample collection Mosquito collection at home

Patient infected by arboviruses

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Major patient
• Patient with clinical signs suggestive of an arbovirus infection:
• Fever And at least 2 clinical signs suggestive of arbovirus among which myalgia, retro-orbital pain, drop in platelets (if data available), digestive symptoms, etc.
• Appearance of clinical signs suggestive of an arbovirus infection in the 7 days preceding the blood sample.
• Patient informed of the performance of the research, the collection of data and biological samples
• Patient who has given their consent to participate in the research by authorizing the collection of data, the collection of an additional blood sample and the use of this sample

You may not qualify if:

• Patient suffering from another identified virosis, an inflammatory disease or taking anti-inflammatory drugs
• Pregnant patient
• Patient returning from a trip within 15 days before the onset of the signs,
• Patient for whom the blood sample is incomplete or could not be performed
• Patient's condition, which, in the doctor's opinion, is incompatible with the additional sample required by the study
• Patient not having consented to participate in the research

Sponsors & Collaborators

• Institut Pasteur: lead
• Institut Pasteur de Nouvelle-Calédonie: collaborator
• Centre Hospitalier Territorial de Nouvelle-Calédonie: collaborator

Study Sites (1)

Centre Hospitalier Territorial de Nouvelle-Calédonie

Dumbéa Sur Mer, New Caledonia

RECRUITING

MeSH Terms

Conditions

Arbovirus Infections; Zika Virus Infection; Dengue; Chikungunya Fever

Condition Hierarchy (Ancestors)

Vector Borne Diseases; Infections; Virus Diseases; Mosquito-Borne Diseases; Flavivirus Infections; Flaviviridae Infections; RNA Virus Infections; Hemorrhagic Fevers, Viral; Alphavirus Infections; Togaviridae Infections

Study Officials

• Myrielle Dupont-Rouzeyrol, PhD

  Institut Pasteur de Nouvelle-Calédonie

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Myrielle Dupont-Rouzeyrol, PhD

CONTACT

+687 27 75 30; mdupont@pasteur.nc

Catherine Inizan, PhD

CONTACT

+687 27 26 66; cinizan@pasteur.fr

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 18, 2020
• First Posted: November 6, 2020
• Study Start: February 15, 2021
• Primary Completion: February 15, 2026
• Study Completion: February 15, 2026
• Last Updated: May 23, 2024

Record last verified: 2024-05

Locations

NE (1)