Cryptic Bacteria of the Thyroid Tissue as a Possible Cause of the Pathology of This Organ

NCT04552496 | Unknown

• 1 other identifier: 85/PB/2018
• Study Type: observational
• Target: 120
• Locations: 1 country
• Sites: 2

Brief Summary

The presence of cryptic microbes has been widely documented in animal healthy deep tissues. The thyroid gland is an organ specifically exposed to the microbial environment due to its close location to the mouth microbiome. A number of bacterial phenotypes has been detected in the inflamed thyroid gland. A question raises as to whether bacteria have not already been present in the thyroid gland before the clinical symptoms of goiter became evident. A problem in thyroid surgery, relatively uncommon but difficult for control, is prolonged thyroidectomy wound healing with skin flap, gland bed inflammation and fibrosis. The causative bacteria may belong to the strains persistently present in the thyroid gland parenchyma. Our objective is to answer questions: a) do the goiter tissue structures contain bacteria, b) if so, which bacterial phenotypes can be identified, c) what are the genetic similarities of the thyroid and periodontal bacterial strains. Studies are carried out in patients with non-toxic multinodular goiter, toxic multinodular goiter, Graves' disease, single adenoma, Hashimoto's disease, thyroid cancer and recurrent thyroid disease. Tissue harvested during surgery is dissected immediately after thyroidectomy into fragments of parenchyma, arteries, veins and lymph nodes and cultured on Columbia blood agar base for up to 3 weeks. In this method bacteria present in the tissue grow in their natural environment, slowly proliferate and then form the on-plate colonies. It enables detection of even single bacteria usually difficult to be identified in planktonic media. Identification of the isolated bacteria is performed. Their DNA patterns are also compared.

Conditions

Bacteria Infection Mechanism

Outcome Measures

Primary Outcomes (5)

• In vivo transferred to ex vivo bacteria culturing in thyroid tissue fragments. The percentage of positive bacterial growth

  Thyroid tissue specimens placed on Columbia agar with sheep blood plate and cultured for up to 30 days. Measurement of the percentage of positive bacterial growth.

  30 days

• In vivo transferred to ex vivo bacteria culturing in thyroid tissue fragments. Time lapse to the first bacterial colonies appearance

  Thyroid tissue specimens placed on Columbia agar with sheep blood plate and cultured for up to 30 days. Optical assessment of colonies growth kinetic. Measurement of time lapse in days to the first bacterial colonies appearance.

  30 days

• Identification of bacterial strains isolated from cultured thyroid tissue fragments

  Isolates identification by standard procedures using the Analytical Profile Identification (API) System (Biomerieux). Assessment of the percentage of bacterial strains cultured from thyroid fragments.

  3 days

• Antibiotic sensitivity of bacterial strains isolated from cultured thyroid tissue fragments

  Assessment of the sensitivity of isolated bacterial strains to antibiotics using the ATB system and the ATB-Plus reader (Biomerieux, Paris, France). The percentage of isolated strains sensitive to tested antibiotics.

  4 days

• Isolated bacteria Polymerase Chain Reaction Melting Profiles (PCR MP)

  The comparison of DNA patterns of strains isolated from thyroid and oral cavity. The analysis of similarity of the genetic pattern as percentage using the GeneTools program (Syngene, Cambridge, United Kingdom).

  3 days

Eligibility Criteria

• Age: 18 Years - 80 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

All patients with thyroid disease who require surgery. Preoperative clinical diagnosis: 1) non-toxic multinodular goiter, 2) toxic multinodular goiter, 3) Graves disease, 4) single adenoma, 5) Hashimoto's disease, 6) thyroid cancer and 7) recurrent thyroid disease.

You may qualify if:

• thyroid disease requiring surgery

You may not qualify if:

• acute or chronic infection at remote sites
• treated with antibiotics over the last 3 months

Sponsors & Collaborators

• Centre of Postgraduate Medical Education: lead

Study Sites (2)

Department of General, Oncological and Gastrointestinal Surgery, Centre of Postgraduate Medical Education

Warsaw, 00-416, Poland

RECRUITING

Department of Applied Physiology, Mossakowski Medical Research Centre, Polish Academy of Sciences

Warsaw, 02-106, Poland

RECRUITING

Study Officials

• Sergiusz Durowicz, MD, PhD

  Centre of Postgraduate Medical Education, Warsaw, Poland

  PRINCIPAL INVESTIGATOR

• Marzanna Zaleska, PhD

  Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

  PRINCIPAL INVESTIGATOR

• Waldemar L. Olszewski, MD, PhD

  Central Clinical Hospital Ministry Interior Administration, Warsaw, Poland

  PRINCIPAL INVESTIGATOR

• Wiesław Tarnowski, Md, PhD

  Centre of Postgraduate Medical Education, Warsaw, Poland

  PRINCIPAL INVESTIGATOR

• Ewa Swoboda-Kopeć, MD, PhD

  Medical University of Warsaw, Warsaw, Poland

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Sergiusz Durowicz, MD, PhD

CONTACT

+48226217173; sdurowicz@wp.pl

Waldemar L. Olszewski, MD, PhD

CONTACT

waldemar.l.olszewski@gmail.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: September 2, 2020
• First Posted: September 17, 2020
• Study Start: July 5, 2018
• Primary Completion: September 1, 2020
• Study Completion: September 1, 2022
• Last Updated: September 17, 2020

Record last verified: 2020-09

Locations

PL (2)