NCT04405492

Brief Summary

Prospective study for clinical performance evaluation of COVID-19 diagnostic tests: detection of anti-SARS-CoV-2 antibodies by RDTs or ELISA (manual or automated), rapid diagnostic tests based on antigen detection, molecular or proteomic testing of SARS-CoV-2 (sensitivity, specificity, predictive values)

Trial Health

35
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
1,210

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started May 2020

Shorter than P25 for not_applicable

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 19, 2020

Completed
6 days until next milestone

Study Start

First participant enrolled

May 25, 2020

Completed
3 days until next milestone

First Posted

Study publicly available on registry

May 28, 2020

Completed
10 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 25, 2021

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 25, 2021

Completed
Last Updated

May 28, 2020

Status Verified

May 1, 2020

Enrollment Period

10 months

First QC Date

May 19, 2020

Last Update Submit

May 25, 2020

Conditions

Outcome Measures

Primary Outcomes (1)

  • Clinical performance of several diagnostic tests : tests based either on detection of the virus SARS-CoV-2 or detection of human antibodies generated in response to infection

    * Tests based on detection of the virus SARS-CoV-2 (antigenic rapid tests, molecular tests, proteomic tests): sensibility and specificity compared to RT-PCR (Ct) in salivary samples and nasopharyngeal swabs from patients and cargivers included in the study * Tests based on detection of human antibodies generated in response to infection (rapid immunodiagnostic tests, ELISA): sensibility and specificity compared to RT-PCR (Ct) and ELISA (antibody titer) in blood or serum from patients and cargivers included in the study

    Total duration of the study is 10 months

Secondary Outcomes (5)

  • Sensitivity and specificity of immunological, antigenic, molecular and proteomic tests according to symptom duration, stage of the disease: asymptomatic, ambulatory, hospitalized, ventilated

    Total duration of the study is 10 months

  • Description of the incidence of infection among hospital caregivers, time to seroconversion according to clinical form, medium-term antibody persistence

    Caregivers will be included in the study for 9 months. After inclusion (Day 0), follow-up visits at Day 15, 1 Month, 3 Months, 6 Months, 9 Months will be done.

  • Sensitivity and specificity of immunodiagnostic tests according to the sample used (serum and capillary blood) and according to the duration of the symptoms

    Total duration of the study is 10 months

  • Sensitivity and specificity of antigenic rapid tests, molecular tests, proteomic tests according to the sample used (salivary samples or nasopharyngeal swabs) and according to the duration of the symptoms

    Total duration of the study is 10 months

  • Suitability of rapid tests in view of its intended purpose for self-testing

    4 months

Study Arms (3)

Population 1 : Patients

EXPERIMENTAL

Hospitalized patients, positive or suspected of SARS-CoV-2 infection

Device: Taking blood samples (capillary and venous), saliva sampling and nasopharyngeal sampling.

Population 2 : Hospital caregivers exposed to SARS-CoV-2

EXPERIMENTAL

Longitudinal study of a hospital caregiver cohort

Device: Taking blood samples (capillary and venous), saliva sampling and nasopharyngeal sampling.

Population 3 : Lay users

EXPERIMENTAL

Suitability of rapid test in view of its intended purpose for self-testing

Device: Capillary and salivary sampling

Interventions

After inclusion, demographic and medical data will be collected. Evaluated point-of-care devices will be tested immediately. Blood samples (capillary and venous), salivary sample and nasopharyngeal swab will be taken. Clinical samples will be stored in a dedicated biobank. Other SARS-CoV-2 diagnostic tests will be evaluated in the laboratory on the different clinical samples retrieved from the biobank. For all antigenic, molecular and serological tests evaluated, the reference method will be real-time RT-PCR as well as the result of medical imaging carried out as part of patient care.

Population 1 : Patients

Two rapid tests (NG Biotech) allowing anti-SRAS-CoV-2 antibodies detection from a fingerpick blood sample and antigen detection from a salivary sample will be provided in 3 pharmacies of Ile de France. The inclusion of lay persons will be carried out in pharmacies by investigators of the recruiting centers (Bacteriology and Virology departments). After inclusion, one of the tests will be given to the participant for self-testing. After performing the test, the participant will complete a questionnaire to give a feedback on the test usability and the result obtained.

Population 3 : Lay users

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may not qualify if:

  • Common criteria for the 3 populations:
  • \- Person subject to a justice protection measure
  • Population 1 (Patients):
  • Refusal to participate by the patient / support person or close friend
  • Patient not speaking French and not accompanied by a translator
  • Patient under guardianship or curatorship
  • Person under AME (State medical aid)
  • Population 2 (Caregivers):
  • \- Refusal to participate
  • Population 3 (lay users):
  • Refusal to participate
  • Person not speaking French and not accompanied by a translator
  • Person under AME (State medical aid)
  • Person out of state to consent, under guardianship or curatorship
  • Pregnant and lactating women

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

COVID-19

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Central Study Contacts

Anne-Marie Roque-Afonso

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 19, 2020

First Posted

May 28, 2020

Study Start

May 25, 2020

Primary Completion

March 25, 2021

Study Completion

March 25, 2021

Last Updated

May 28, 2020

Record last verified: 2020-05