Follicular Fluid Exosome miRNA During Oocyte Maturation

NCT04382872 | Recruiting

• 1 other identifier: 2019.137
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Folliculogenesis involves the recruitment of primordial follicles into a group of growing follicles in which eventually one follicle is selected, matured and ovulated. Complex endocrine and intra-ovarian paracrine interactions occur to create a changing intra-follicular hormonal milieu suitable for oocyte development. However, this oocyte developmental competence and to undergo fertilization and embryogenesis is impaired in aged women. Diminished ovarian function is generally attributed to decreased quantity and quality of oocytes and their surrounding granulosa cells during ovarian aging, although the underlying mechanisms remain unclear. The aim of the study is to investigate the miRNA in (follicular fluid) FF exosome in young and aged women and their relationship to egg maturation.

Conditions

Oocyte Maturation

Keywords

oocyte maturation; in vitro maturation

Outcome Measures

Primary Outcomes (2)

• RNA-sequencing

  1\) To identify for the first time FF exosome miRNAs in mature and immature follicles in young and aged women by RNA-sequencing (RNA-seq).

  through study completion, an average of 2 years

• quantitative real-time polymerase chain reaction

  2\) To verify the identified FF exosome miRNAs in mature and immature follicles in young and aged women and investigate their expression in the corresponding granulosa cells by quantitative real-time polymerase chain reaction (qRT-PCR).

  through study completion, an average of 2 years

Secondary Outcomes (1)

• fertilization and pregnancy rate

  through study completion, an average of 2 years

Eligibility Criteria

• Age: 20 Years - 45 Years
• Gender Eligibility Details: this study required the subject to provide follicular fluid and immature oocyte, and therefore the subjects must be female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

women undergoing oocyte retrieval treatment

You may qualify if:

• Women aged 20 to 45;
• BMI \> 19 \& \< 35;
• Women who is planning to perform invitro fertilization (IVF) together with intracytoplasmic sperm injection (ICSI) for their fertility problems

You may not qualify if:

• Positive pregnancy test before taking study medications;
• Women with BMI\>35 kg/m2;
• Women with known chromosomal translocation, recurrent abortions or those carrying Fragile X;
• Women with endometriosis

Sponsors & Collaborators

• Chinese University of Hong Kong: lead

Study Sites (1)

The Chinese University of Hong Kong

Hong Kong, Hong Kong

RECRUITING

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: April 23, 2020
• First Posted: May 11, 2020
• Study Start: April 30, 2021
• Primary Completion: April 30, 2026
• Study Completion: April 30, 2026
• Last Updated: August 13, 2025

Record last verified: 2025-08

Locations

HK (1)