Avenanthramide and Saponin Bioavailability in Oat Bran

NCT04335435 | Completed

• 1 other identifier: 15-0179
• Study Type: interventional
• Target: 13
• Locations: 0 countries
• Sites: N/A

Brief Summary

Avenanthramides and saponins are types of chemical compounds found naturally in oats. Avenanthramides have anti-oxidant properties, anti-atherosclerotic, anti-inflammation, and anti-proliferative effects on cancer cells in vitro. Oat saponins, or avenacosides, have the ability to bind cholesterol, and thus, the ability to lower blood cholesterol. Oat bran is a known source of these dietary compounds. This study aims to determine the bioavailability of these compounds to in the urine of participants after ingesting an oat bran cereal, both before, and after for multiple time points. Analytical chemistry will be used to determine the bioavailability of the oat compounds at each time point. This will help to establish a kinetic curve for the metabolism of these compounds.

Conditions

Human Health; Polyphenols; Pharmacokinetics

Keywords

Avenanthramides; Saponins; bioavailability; polyphenols; oat bran; pharmacokinetics

Outcome Measures

Primary Outcomes (8)

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  0-2 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  2-4 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  4-6 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  6-9 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  9-12 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  12-24 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  24-32 hours post-dose

• Bioavailability of Avenanthramides and Saponins in Urine

  Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.

  32-48 hours post-dose

Study Arms (1)

Oat Bran Consumption

EXPERIMENTAL

Each subject consumed 120 g of oat bran (by dry weight) in a single dose, and samples (urine and fecal) were collected at different time points following the administration of oat bran.

Dietary Supplement: Oat Bran Consumption

Interventions

Oat Bran Consumption DIETARY_SUPPLEMENT

Oat bran (120 g), single dose.

Also known as: Gut Microbiome and Oat metabolism Study

Oat Bran Consumption

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• BMI 18.5-25
• Have blood and urine biochemical markers in normal range
• Have no known allergy to oat relate foods
• Be not taking antibiotics for six months
• Be not currently taking medication
• Be nonsmoking
• Have no alcoholic intoxication
• Have no extensive exposure to industrial waste

You may not qualify if:

• Individuals with gout
• Individuals with heart disease
• Individuals with peripheral vascular diseases
• Individuals with degenerative liver
• Cancer patients
• Patients with diabetes
• Individuals with GI disorders
• Individuals with endocrine disorders

Sponsors & Collaborators

• North Carolina Agriculture & Technical State University: lead

Related Publications (1)

• Wang P, Zhang S, Yerke A, Ohland CL, Gharaibeh RZ, Fouladi F, Fodor AA, Jobin C, Sang S. Avenanthramide Metabotype from Whole-Grain Oat Intake is Influenced by Faecalibacterium prausnitzii in Healthy Adults. J Nutr. 2021 Jun 1;151(6):1426-1435. doi: 10.1093/jn/nxab006.

  PMID: 33694368 DERIVED

MeSH Terms

Interventions

Gastrointestinal Microbiome

Intervention Hierarchy (Ancestors)

Microbiota; Microbiological Phenomena; Biota; Biodiversity; Ecosystem; Environment; Ecological and Environmental Phenomena; Biological Phenomena; Environment and Public Health

Study Officials

• Shengmin Sang, PhD

  North Carolina Agriculture and Technical State University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: April 2, 2020
• First Posted: April 6, 2020
• Study Start: January 15, 2016
• Primary Completion: January 21, 2016
• Study Completion: January 21, 2016
• Last Updated: August 6, 2020

Record last verified: 2020-08