NCT04212728

Brief Summary

The purpose of this study is to explore the efficacy and safety of autologous adipose-derived mesenchymal stem cells (AMSCs) plus autologous platelet rich plasma (PRP) in the treatment of severe knee osteoarthritis.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
60

participants targeted

Target at P50-P75 for not_applicable knee-osteoarthritis

Timeline
Completed

Started Dec 2019

Longer than P75 for not_applicable knee-osteoarthritis

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

December 26, 2019

Completed
1 day until next milestone

First Posted

Study publicly available on registry

December 27, 2019

Completed
2 days until next milestone

Study Start

First participant enrolled

December 29, 2019

Completed
3.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2023

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2024

Completed
Last Updated

March 28, 2023

Status Verified

March 1, 2023

Enrollment Period

3.9 years

First QC Date

December 26, 2019

Last Update Submit

March 26, 2023

Conditions

Outcome Measures

Primary Outcomes (2)

  • Physical function change

    Evaluation the physical function change measured by the Western Ontario and McMaster Universities Arthritis Index (WOMAC). The WOMAC consists of three subscales: pain (five questions), stiffness (two questions), and physical function (17 questions). The subscale scores can vary, with pain ranging from 0 to 20 points; stiffness, 0 to 8 points; and physical function, 0 to 68 points. Higher scores represent worse pain, stiffness, and functional limitations.

    From before randomization until 3, 6, and 12 months after treatment start.

  • Change in pain density

    Evaluation the changing of pain density measured by Visual Analogue Scale. Draw a 10 cm horizontal line on the paper. One end of the line is 0, indicating no pain; the other end is 10, indicating severe pain; and the middle part indicates varying degrees of pain. The patient selects a point from the horizontal line and the length from 0 to this point is the Visual Analogue Scale score.

    From before randomization until 3, 6, and 12 months after treatment start.

Secondary Outcomes (4)

  • Cartilage repair

    From before randomization until 3, 6, and 12 months after treatment start.

  • Change in MOS item short from health survey(SF-36)

    From before randomization until 3, 6, and 12 months after treatment start.

  • Change in Lequesne Index

    From before randomization until 3, 6, and 12 months after treatment start.

  • Change in knee society score (KSS)

    From before randomization until 3, 6, and 12 months after treatment start.

Study Arms (2)

AMSCs plus PRP group

EXPERIMENTAL

Three intra-articular injections in total and autologous adipose-derived mesenchymal stem cells (AMSCs) suspended in 3 ml autologous platelet rich plasma (PRP) for each injection. Time-points for intervention: 1) initial injection; 2) 3 months following initial injection; 3) 6 months following initial injection.

Biological: Auotologous AMSCs plus autologous PRP

PRP group

ACTIVE COMPARATOR

Three intra-articular injections in total and 3 ml autologous platelet rich plasma (PRP) for each injection. Time-points for intervention: 1) initial injection; 2) 3 months following initial injection; 3) 6 months following initial injection.

Biological: Auotologous PRP

Interventions

150 mL subcutaneous abdominal adipose tissue will be harvested via liposuction . AMSCs will be isolated and cultured from adipose tissue. Before injection, 30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.Cultured AMSCs will be collected and suspended by 3ml autologous PRP.

AMSCs plus PRP group
Auotologous PRPBIOLOGICAL

30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.

PRP group

Eligibility Criteria

Age40 Years - 70 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Subject has Kellgren and Lawrence grade II-IV primary osteoarthritis as determined by X-ray.
  • Subject's pain score is 8-13 points (Lequesne's index).
  • Ages between 40-70 years.
  • Signed informed consent from the subject.

You may not qualify if:

  • Subject infected with hepatitis B, hepatitis C, HIV, syphilis or HTLV
  • Subject not suitable for liposuction surgery.
  • Subject with hypersensitivity/allergy to anesthetic.
  • Subject's creatinine values higher than 1.6mg/dl.
  • Subject with body mass index, BMI over 30.
  • Subject's studied knee treated with intra-articular injection therapy within 6 months prior to screen.
  • Subject has undergone surgery on studied knee, including fracture surgery, arthroscopic surgery, meniscus repair surgery, or cruciate ligament reconstruction surgery.
  • Subject enrolled in any other cell therapy studies within the past 30 days.
  • Subject who the investigator considers inappropriate for the clinical trial due to any other reasons than those listed above.
  • Subject has a history of gouty arthritis, septic arthritis, rheumatoid arthritis and any other autoimmune arthritis of the knee joint.
  • Subject has had major medical problems in vital organs, such as; heart, liver, kidney, or lung.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Yantai Yuhuangding Hospital

Yantai, Shandong, 264000, China

RECRUITING

MeSH Terms

Conditions

Osteoarthritis, Knee

Condition Hierarchy (Ancestors)

OsteoarthritisArthritisJoint DiseasesMusculoskeletal DiseasesRheumatic Diseases

Study Officials

  • Peiwen Lian, PhD

    Yantai Yuhuangding Hospital

    STUDY DIRECTOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Peiwen Lian Ph.D

Study Record Dates

First Submitted

December 26, 2019

First Posted

December 27, 2019

Study Start

December 29, 2019

Primary Completion

December 1, 2023

Study Completion

December 1, 2024

Last Updated

March 28, 2023

Record last verified: 2023-03

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