Analysis of Bacterial Microbiome of Endodontically Infected Primary and Permanent Teeth

NCT04211519 | Completed

• 1 other identifier: 2017/234
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Recognition of community profiles in endodontic infections may allow a better understanding of the pathogenesis of the disease and the establishment of more effective treatment protocols. Therefore, the aim of the present study was to investigate bacterial diversity in endodontically infected primary and permanent teeth using 16S rRNA gene sequencing and QIIME 2TM (Quantitative Insights Into Microbial Ecology 2) bioinformatics pipeline

Conditions

Endodontic Disease; Endodontic Inflammation

Keywords

Endodontic disease; Microbiome; Permanent teeth; Primary teeth

Outcome Measures

Primary Outcomes (1)

• Comparison of microbiome composition of endodontically infected primary and permanent tooth canal

  Analysis of the profile of microbial populations based on 16S rRNA gene analysis in endodontically infected permanent and primary teeth

  6 months

Study Arms (2)

Permanent teeth group

EXPERIMENTAL

For the disinfection of teeth, 30% hydrogen peroxide and 2.5% sodium hypochlorite solution were used for 30 seconds each. Then, 5% sodium thiosulfate solution was used to inactivate the disinfectant agents. Cavity preparation and root canal access were accomplished using sterile high-speed diamond burs under water cooling. Microbial samples were taken immediately by the same researcher from the largest root canal under strict aseptic conditions by using paper point method. Sterilized minimum four paper points were placed to the same level in root canal and the root canal content was absorbed. Each paper point was kept into the canal for at least 30 seconds. Then, paper points were placed into the Eppendorf tubes and refrigerated at -80 °C within 10 min.

Diagnostic Test: sampling

Primary teeth group

EXPERIMENTAL

For the disinfection of teeth, 30% hydrogen peroxide and 2.5% sodium hypochlorite solution were used for 30 seconds each. Then, 5% sodium thiosulfate solution was used to inactivate the disinfectant agents. Cavity preparation and root canal access were accomplished using sterile high-speed diamond burs under water cooling. Microbial samples were taken immediately by the same researcher from the largest root canal under strict aseptic conditions by using paper point method. Sterilized minimum four paper points were placed to the same level in root canal and the root canal content was absorbed. Each paper point was kept into the canal for at least 30 seconds. Then, paper points were placed into the Eppendorf tubes and refrigerated at -80 °C within 10 min.

Diagnostic Test: sampling

Interventions

sampling DIAGNOSTIC_TEST

sampling from endodontically infected primary and permanent teeth by using paper point method

Permanent teeth group; Primary teeth group

Eligibility Criteria

• Age: 4 Years - 13 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• have intact roots or \<1/3 of physiological root resorption
• have clinical crowns that permit effective rubber dam isolation
• no mobility, fistula, pus discharge, gingival swelling, periapical abscess or internal resorption.

You may not qualify if:

• have marginal periodontitis, a history of pharmacological treatment, antibiotics or fluoride intake within the last 2 months
• a history of cancer, diabetes or immunodeficiency disorders

Sponsors & Collaborators

• Nuh Naci Yazgan University: lead
• Mersin University: collaborator

Study Sites (1)

Nuh Naci Yazgan Üniversitesi

Kayseri, Kocasinan, 38170, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Dental Pulp Diseases; Pulpitis

Condition Hierarchy (Ancestors)

Tooth Diseases; Stomatognathic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: DIAGNOSTIC
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Ebru Delikan

Study Record Dates

• First Submitted: December 24, 2019
• First Posted: December 26, 2019
• Study Start: September 1, 2017
• Primary Completion: February 15, 2018
• Study Completion: December 10, 2019
• Last Updated: December 26, 2019

Record last verified: 2019-12

Locations

TU (1)