Breaking-up Sedentary Time and 2 Weeks Detraining

NCT04142346 | Completed DMC

• 1 other identifier: CEIFMH6/2019
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

Regularly interrupting sedentary behavior (SB) with activity breaks may attenuate postprandial glucose (PPG) excursions and improve glycemic control. The investigators aimed to determine the effect of interrupting 7 hours of prolonged sitting with brief bouts of moderate physical activity (PA) (alternating between up/down stairs and sit/stand up from the chair) on postprandial glucose (PPG) responses in comparison with uninterrupted sitting. In addition, the investigators aimed to examine the effects of 2 weeks of detraining (DT) on PPG on both protocols. Non-diabetic, trained older adults (n = 15) will be recruited for a randomized crossover trial with two treatments performed in two different training conditions: 1) uninterrupted sitting protocol (CON); 2) seated with 2-minutes bouts of moderate PA every 30 minutes (INT). Both protocols will be performed in a trained condition and after 2 weeks of DT. In the early morning of each trial, participants will do an oral glucose tolerance test (OGTT) and 2 blood samples will be collected (fasting and after 2 hours); 2.5 hours after, participants will begin the protocol and two standardized meals will be provided (0 hours and at 3 hours). An iPro2 continuous glucose monitoring (CGM) system will record the average interstitial glucose concentration every 5 minutes. Positive incremental area under the curve (iAUC) and total area under the curve (pAUC) for glucose as well as mean glucose (MG) will be calculated using Matlab. Differences between both protocols and between the two different moments will be examined using generalized estimation equation (GEE), adjusting for sex and age (CI 95%).

Conditions

Old Age

Keywords

sitting time; physical inactivity; breaks; glycemic control; continuous glucose monitoring; older adults

Outcome Measures

Primary Outcomes (1)

• Continuous glucose monitoring before and after 2-Weeks of detraining for each intervention protocol (prolonged sitting vs. breaking-up sitting)

  Immediately upon arrival at the laboratory and throughout 24h, a blood glucose monitor (iPro2 CGM with Enlite Sensors MiniMed; Medtronic, Northridge, California, USA) will be placed on the right side of the abdomen. Once inserted, CGM provides interstitial glucose values every 5 minutes. To calibrate the CGM, four capillary blood glucose samples (three in the laboratory and one at home) will be collected and measured using a glucometer (Contour Next One, Ascensia Diabetes Care, Basel, Switzerland).

  1-month (2 measurement times, one for each protocol, including baseline and after detraining)

Secondary Outcomes (12)

• Postural allocation

  1-month (2 measurement times, one for each protocol, including baseline and after detraining)

• Body Composition

  1-month (2 measurement times (one at baseline and after two weeks of detraining))

• Weight

  1-month (2 measurement times (one at baseline and after two weeks of detraining))

• Height

  1-month (2 measurement times (one at baseline and after two weeks of detraining))

• Cardiorespiratory fitness (CRF)

  1-month (2 measurement times (one at baseline and after two weeks of detraining))

Study Arms (2)

Uninterrupted sitting

NO INTERVENTION

Participants remained seated throughout 7 hours. During the protocol, participants were allowed to go to the bathroom in a wheelchair and al-libitum water was granted.

Sitting + moderate intensity breaks

EXPERIMENTAL

Participants were instructed to sit throughout 7 hours, while interrupting the sitting position every 30 minutes to perform 2 minutes of moderate-intensity physical activity. The breaks consisted of walk up and down stairs and squats. Each person performed these exercise alternately. During the protocol, participants were allowed to go to the bathroom in a wheelchair and al-libitum water was granted.

Behavioral: Sitting + moderate intensity breaks

Interventions

Sitting + moderate intensity breaks BEHAVIORAL

Participants will be followed in a randomized crossover trial undertaken at Faculty of Human Kinetics between April and August 2019. Participants will attend to the laboratory on twelve separate occasions: visit 1 (day 1), baseline assessments for body composition; visit 2 (day 2), cardiorespiratory fitness assessment; visit 3 (day 3), OGTT and the first randomized experimental protocol; visit 4 (day 4), follow-up OGTT; visit 5 (4-6 days after the first experimental protocol), OGTT and the remaining experimental protocol; visit 6 (day following the previous experimental protocol), follow-up OGTT. An additional 6 visits will be performed following the same rational, after a 2-week period of detraining, where participants will be instructed to refrain from structured exercise. The follow-up assessments for body composition and cardiorespiratory fitness will be accomplished after the protocols.

Sitting + moderate intensity breaks

Eligibility Criteria

• Age: 65 Years - 90 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Older Adult (65+)

You may qualify if:

• Men or women
• and 90 years-old
• Physically active
• Engaged in structured exercise at least twice a week for the past 6 months.

You may not qualify if:

• Diagnosed with diabetes
• Any type of physical limitation that would prevent them from practicing exercise

Sponsors & Collaborators

• University of Lisbon: lead
• Associação Protectora dos Diabéticos de Portugal: collaborator

Study Sites (1)

Faculty of Human Kinetics, University of Lisbon

Cruz Quebrada, Portugal

Location

MeSH Terms

Conditions

Sedentary Behavior

Interventions

Sitting Position

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Posture; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Luis Sardinha, PhD

  Professor

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: October 21, 2019
• First Posted: October 29, 2019
• Study Start: April 1, 2019
• Primary Completion: August 31, 2019
• Study Completion: August 31, 2019
• Last Updated: October 30, 2019

Record last verified: 2019-10

Locations

PT (1)