Lysine Metabolic Availability in Children

NCT04135040 | Completed

• 1 other identifier: H17-00452
• Study Type: interventional
• Target: 5
• Locations: 1 country
• Sites: 1

Brief Summary

This study will evaluate how much of the essential amino acid lysine school-aged children are using for protein synthesis when consuming different cereal grains. Six healthy children between 6-10y will be recruited. They will be given cooked white rice, corn, oats, black beans, and milk. Using a minimally invasive technique, the amount of lysine that is available from cereal grain products will be determined. With the results from this research project accurate diet recommendations will be developed for children consuming cereal-based diets.

Conditions

Children

Keywords

IAAO; Lysine; Children; Cereal Grains

Outcome Measures

Primary Outcomes (1)

• 13 CO2 production

  Breath samples will be collected during the study day, to measure the rate of oxidation of tracer phenylalanine in the expired breath.

  8 hours (1 study day)

Secondary Outcomes (1)

• Urinary lysine

  8 hours, 2 samples at the beginning and end of the study day.

Study Arms (1)

Lysine metabolic availability

EXPERIMENTAL

Lysine metabolism from pure amino acids and cereal foods in children.

Dietary Supplement: L-Amino Acid

Interventions

L-Amino Acid DIETARY_SUPPLEMENT

8 different lysine intakes (L-Amino Acid and food) will be tested for 8 hours each.

Lysine metabolic availability

Eligibility Criteria

• Age: 6 Years - 10 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)

You may qualify if:

• Healthy school-aged children between the ages of 6-10y.
• Normal weight.
• Normal eating habits.

You may not qualify if:

• Children under 6 years old, or over 10 years old.
• Children who are currently ill (fever, cold, vomiting or diarrhea).
• Children outside normal weight parameters (3rd - 85th percentiles for weight).
• Children with claustrophobia.
• Children currently or recently taking medication or antibiotics.
• Children with food allergies.

Sponsors & Collaborators

• University of British Columbia: lead
• Canadian Institutes of Health Research (CIHR): collaborator

Study Sites (1)

BC Children's Hospital Research Institute

Vancouver, British Columbia, V5Z4H4, Canada

Location

Related Publications (1)

• Caballero K, Mandal R, Pratap-Singh A, Kitts DD, Ball RO, Pencharz PB, Courtney-Martin G, Elango R. Lysine Bioavailability in School-Age Children Consuming Rice Is Reduced by Starch Retrogradation. J Nutr. 2020 Dec 10;150(12):3208-3215. doi: 10.1093/jn/nxaa276.

  PMID: 33025006 DERIVED

Study Officials

• Rajavel Elango, PhD

  University of British Columbia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: June 4, 2018
• First Posted: October 22, 2019
• Study Start: June 1, 2017
• Primary Completion: April 24, 2021
• Study Completion: December 5, 2021
• Last Updated: July 19, 2024

Record last verified: 2024-07

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)