NCT04110366

Brief Summary

Intranasal live attenuated influenza vaccine (LAIV; trade name FluMist/Fluenz-Tetra, manufactured by AstraZeneca/Medimmune) is the standard influenza vaccine given to children aged 2-17 years of age in the UK. It is also licensed to be given to adults up to the age of 49 years in the USA. The systems biology of the human blood response to influenza vaccines has been studied in great detail, but there is a paramount need to study innate and specific, soluble and cellular immune responses at the nasal mucosal site of influenza infection. In this way this study aims to determine correlates of efficacy and vaccine take in serum and nasal mucosal lining fluid (MLF).

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
48

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jun 2018

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 14, 2018

Completed
10 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 4, 2019

Completed
5 months until next milestone

First Submitted

Initial submission to the registry

September 9, 2019

Completed
22 days until next milestone

First Posted

Study publicly available on registry

October 1, 2019

Completed
8 months until next milestone

Study Completion

Last participant's last visit for all outcomes

May 29, 2020

Completed
1.5 years until next milestone

Results Posted

Study results publicly available

December 6, 2021

Completed
Last Updated

December 6, 2021

Status Verified

November 1, 2021

Enrollment Period

10 months

First QC Date

September 9, 2019

Results QC Date

August 26, 2021

Last Update Submit

November 1, 2021

Conditions

Keywords

influenzavaccinevirusantibodycytokinerespiratory

Outcome Measures

Primary Outcomes (1)

  • Number of Participants Shedding Each Vaccine Virus Measured by qPCR of Nasosorption Samples

    Vaccine virus shedding in nasosorption samples collected between 1-7 days post-vaccination and quantified using multiplex qPCR assay measures in the LAIV vaccine recipient cohort.

    1-7 days post vaccination

Secondary Outcomes (1)

  • Number of Participants With >2-fold Rise in Mucosal and/or Serum Antibody Titre Against Each Vaccine Virus Haemagglutinin Antigens

    28 days post vaccination

Study Arms (2)

Live attenuated influenza vaccine

EXPERIMENTAL

Participants receiving live attenuated influenza vaccine (LAIV)

Biological: Live attenuated influenza vaccine

Mucosal immune stability cohort

EXPERIMENTAL

Participants receiving a vehicle control nasal challenge

Other: Vehicle control

Interventions

Vaccination with live attenuated influenza vaccine (LAIV)

Also known as: Fluenz
Live attenuated influenza vaccine

Vehicle control nasal challenge

Mucosal immune stability cohort

Eligibility Criteria

Age18 Years - 30 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Capacity to provide written informed consent
  • Aged 18-30 years (inclusive)
  • Fluent English speaker

You may not qualify if:

  • Current involvement in another study unless observational or in follow-up phase (non-interventional)
  • Received any influenza vaccine over the last 2 years
  • Egg allergy
  • Previous significant adverse reaction to any vaccination/immunisation
  • Current regular (daily) smoker
  • Pregnant
  • Any medication that may affect the immune system (e.g. steroids)
  • Taking regular acetylsalicylic acid (aspirin)
  • Unable to give informed consent
  • Current acute severe febrile illness
  • Taking long term antibiotics
  • Clinically diagnosed influenza in the last 2 years
  • Any long-term health problem with heart disease, lung disease (including asthma), kidney disease, neurologic disease, liver disease, metabolic disease (e.g. diabetes) or anemia or another blood disorder
  • Use of drugs for the treatment of rheumatoid arthritis, Crohn's disease, or psoriasis or anticancer drugs; or radiation treatments
  • History of Guillain-Barre syndrome
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Imperial Clinical Respiratory Research Unit

London, W2 1PG, United Kingdom

Location

Related Publications (1)

  • Thwaites RS, Uruchurtu ASS, Negri VA, Cole ME, Singh N, Poshai N, Jackson D, Hoschler K, Baker T, Scott IC, Ros XR, Cohen ES, Zambon M, Pollock KM, Hansel TT, Openshaw PJM. Early mucosal events promote distinct mucosal and systemic antibody responses to live attenuated influenza vaccine. Nat Commun. 2023 Dec 5;14(1):8053. doi: 10.1038/s41467-023-43842-7.

MeSH Terms

Conditions

Influenza, HumanVirus Diseases

Interventions

Influenza Vaccines

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsRespiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Viral VaccinesVaccinesBiological ProductsComplex Mixtures

Results Point of Contact

Title
Dr Ryan Thwaites
Organization
Imperial College London

Study Officials

  • Peter J Openshaw, PhD

    Imperial College London

    PRINCIPAL INVESTIGATOR
  • Trevor T Hansel, PhD

    Imperial College London

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 9, 2019

First Posted

October 1, 2019

Study Start

June 14, 2018

Primary Completion

April 4, 2019

Study Completion

May 29, 2020

Last Updated

December 6, 2021

Results First Posted

December 6, 2021

Record last verified: 2021-11

Data Sharing

IPD Sharing
Will share

IPD sharing is available upon request to the study PI; but access is offered at the discretion of the PI and requests are not guaranteed access. All access will require ethical approval.

Shared Documents
STUDY PROTOCOL, CSR, ANALYTIC CODE
Time Frame
From June 2020 onward, no set end date
Access Criteria
PI discretion

Locations