Evaluation of Bacterial Flora Among Operation Theatre Staff at a Tertiary Care Hospital

NCT04089683 | Completed

• 1 other identifier: KamineniAMSRC
• Study Type: observational
• Target: 361
• Locations: 0 countries
• Sites: N/A

Brief Summary

Health care associated infection (HCAI) is a serious health hazard as it leads to increased morbidity and mortality of patients, length of hospital stay and costs associated with increased hospital stay. A total of 361 subjects divided into four groups were included in this study. The first group comprised of 179 doctors, the second had 31 nurses, third group had 110 OT Technicians and the fourth group included 41 subjects which included housekeeping staff and cleaners. Swabs were collected from OT staff at the time of entry and at exit from the OT. Places of swabs taken were: A) Web space, B) OT dress and C) Anterior nares.

Conditions

Microbial Colonization

Keywords

Operation Theatre; Bacterial Contamination; Web-space; OT Dress; Anterior Nares

Outcome Measures

Primary Outcomes (3)

• Bacterial flora harboured at Web spaces among OT staff

  Swabs will be taken from web spaces before entering Operation Theatre and while going out of OT at the end of the day and cultured to look for growth of Bacteria

  2 years

• Bacterial load on OT dress among OT staff

  Swabs will be taken from OT dress while donning them and before going out of OT at the end of the day and cultured to look for growth

  2 years

• To identify the nasal carriers of organisms among OT staff

  Swabs will be taken from anterior nares while entering OT at the end of the day while leaving OT and cultured to look for growth

  2 years

Study Arms (4)

Doctors

Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre

Other: Swab stick

Nurses

Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre

Other: Swab stick

OT Technicians

Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre

Other: Swab stick

House keeping staff and cleaners

Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre

Other: Swab stick

Interventions

Swab stick OTHER

Swab sticks collected from Web Spaces, Scrub, Anterior Nares

Doctors; House keeping staff and cleaners; Nurses; OT Technicians

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

All OT staff viz. Surgeons, Anesthetists, Residents, OT matron/Nurse, OT Technicians, Housekeeping and Cleaning staff were include in the study. All the patients in OT and samples from the OT air \& equipment were excluded from the study. All subjects were screened as per inclusion and exclusion criteria and selected without any gender bias. The study population contained 361 subjects among which were divided into four groups. Doctors, Nurses, OT technicians and others. Swabs were collected from OT staff at the time of entry in to OT and at the time of exit from OT. Places of swabs taken are: A) Web space, B) OT dress, C) Anterior nares (distal 1cm in a circular motion). Swabs were inoculated on 5% sheep blood agar/ Mueller-Hinton agar/ Mannitol salted agar/ MacConkey agar and were incubated at 35-37 degree centigrade under aerobic conditions for 48hrs with observation once in 24hrs. The age, gender and rank of the OT staff were collected as a part of demographic data.

Sponsors & Collaborators

• Kamineni Academy of Medical Sciences and Research Centre: lead

MeSH Terms

Conditions

Communicable Diseases

Condition Hierarchy (Ancestors)

Infections; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor, Dept of Surgery, KAMSRC, Hyderabad, India

Study Record Dates

• First Submitted: September 6, 2019
• First Posted: September 13, 2019
• Study Start: April 1, 2014
• Primary Completion: April 1, 2016
• Study Completion: April 1, 2016
• Last Updated: September 13, 2019

Record last verified: 2019-09