NCT04061486

Brief Summary

The goal of this observational study is to compare clinical outcomes of two techniques for sperm cell sorting, microfluidic sorting and magnetic activated cell sorting (MACS) technique, in males with high DNA fragmentation within an assisted reproductive treatment. The main questions it aims to answer are:

  • Does microfluidic sorting improve pregnancy rate compared to MACS?
  • Does microfluidic sorting improve fertilization rate compared to MACS?
  • Does microfluidic sorting improve implantation rate compared to MACS?
  • Does microfluidic sorting improve blastocyst development rate compared to MACS? Participants will deposit an eyaculate sample that will be analyzed for DNA fragmentation using a aliquot, then split in two to perform MACS and the microfluidic sorting. Both will be tested for DNA fragmentation (aliquotes) and used for the ICSI treatment with donor oocytes. Researchers will compare both techniques to see if the selection of the best functional spermatozoa with less DNA fragmentation and clinical outcomes improve.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
84

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Aug 2019

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 8, 2019

Completed
8 days until next milestone

First Submitted

Initial submission to the registry

August 16, 2019

Completed
3 days until next milestone

First Posted

Study publicly available on registry

August 19, 2019

Completed
1.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 15, 2021

Completed
8 months until next milestone

Study Completion

Last participant's last visit for all outcomes

January 9, 2022

Completed
Last Updated

October 4, 2024

Status Verified

October 1, 2024

Enrollment Period

1.8 years

First QC Date

August 16, 2019

Last Update Submit

October 2, 2024

Conditions

Keywords

MACSDNA fragmentationSperm selection

Outcome Measures

Primary Outcomes (1)

  • Pregnancy rate

    Total of positive pregnancies / total of patients with embryo transfer

    Mar 2019 - Oct 2021

Secondary Outcomes (3)

  • Fertilization rate

    Mar 2019 - Oct 2021

  • Implantation rate

    Mar 2019 - Oct 2021

  • Blastocyst rate

    Mar 2019 - Oct 2021

Study Arms (1)

Experimental arm

One half of the oocyte cohort is microinjected with sperm selected by the Fertile technique, while the other half of the patient's oocyte cohort is microinjected with sperm selected by the MACS technique.

Eligibility Criteria

Age18 Years - 50 Years
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64)
Sampling MethodProbability Sample
Study Population

Infertile patients needing an IVF treatment

You may qualify if:

  • Oocyte recipient patients

You may not qualify if:

  • Sperm concentration \< 5 Mill/ml
  • Percentage of motile sperm \< 15%

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

IVI RMA Seville

Seville, 41011, Spain

Location

MeSH Terms

Conditions

Infertility, Male

Condition Hierarchy (Ancestors)

Genital Diseases, MaleGenital DiseasesUrogenital DiseasesInfertilityMale Urogenital Diseases

Study Officials

  • Cristina González Ravina, PhD

    IVI RMA Seville

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
CASE CROSSOVER
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 16, 2019

First Posted

August 19, 2019

Study Start

August 8, 2019

Primary Completion

May 15, 2021

Study Completion

January 9, 2022

Last Updated

October 4, 2024

Record last verified: 2024-10

Locations