Generation of Biological Samples Positive to Somatropin for Anti-doping Control

NCT04042428 | Completed Phase 1

• 1 other identifier: IMIMFTCL/GH/5
• Study Type: interventional
• Target: 6
• Locations: 1 country
• Sites: 1

Brief Summary

Background: Somatropin, also known as recombinant growth hormone (rhGH), is one of the World Anti-Doping Agency (WADA) prohibited substances. Its consumption in athletes has been banned since 1990, as it is known to improve physical performance. Hypothesis: The subcutaneous administration of recombinant somatropin (rhGH) in healthy subjects allows obtaining positive doping samples. The concentrations of hGH variants (isoforms) and biomarkers can be measured in serum. Objectives: Primary objective: To generate enough serum samples positive to recombinant somatropin in order to be analyzed as control samples by anti-doping laboratories. Secondary objective: To determine the analytical parameters necessary to detect the administration of recombinant somatropin in healthy volunteers by direct and/or indirect methods. Methods: Phase I, open, randomized clinical trial, with a treatment condition (recombinant somatropin or rhGH) administered subcutaneously to 4 subjects (2:1 ratio). Control samples of the study correspond to basal samples of 2 subjects who do not receive any treatment.

Conditions

Healthy Volunteers

Keywords

Somatropin; Growth hormone; Anti-doping control; Athletic performance

Outcome Measures

Primary Outcomes (1)

• Seric concentration of rhGH

  Concentration of the pool of molecular variants (isoforms) of somatropin in blood

  6 hours after last administration

Secondary Outcomes (8)

• Changes in the proportion of somatropin isoforms

  6 hours after last administration

• Changes in seric concentration of ghrelin

  6 hours after last administration

• Changes in seric concentration of leptin

  6 hours after last administration

• Changes in seric concentration of adiponectin

  6 hours after last administration

• Changes in seric concentration of type III procollagen (P-III-P)

  6 hours after last administration

Study Arms (2)

Treatment group

EXPERIMENTAL

Subjects receive a 14-day treatment. A 450 mL blood sample is extracted 6 hours after the last administration (day 14).

Drug: Somatropin injection

Control group

NO INTERVENTION

Subjects do not receive any treatment. A 450 mL blood sample is extracted at baseline.

Interventions

Somatropin injection DRUG

Subjects receive a daily subcutaneous dose of 0.2 IU/kg (0.067 mg/kg) of recombinant somatropin (rhGH) during 14 days.

Treatment group

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male volunteers between 20 and 30 years old.
• History and physical examination that demonstrate not presenting organic or psychiatric disorders.
• ECG, blood and urine tests performed before the trial within normal limits. Minor or punctual variations of these limits will be allowed if, in the opinion of the principal investigator, they have no clinical significance, do not pose a risk to the subject and do not interfere in the evaluation of the product. These variations and their non-relevance will be justified in writing.
• Body mass index \[BMI: weight/height\^2\] between 19 and 26, and weight between 50 and 90 kg.
• Able to understand and accept the trial procedures and sign an informed consent.

You may not qualify if:

• Having suffered some organic disease or major surgery during the three months prior to the trial.
• Suffering any type of illness, acute or chronic at the time of the study.
• Background or clinical evidence of cardiovascular, respiratory, renal, hepatic, endocrine, gastrointestinal, hematological, neurological, dermatological, or other acute or chronic diseases that, in the opinion of the Principal Investigator or the collaborators designated by it, may pose a risk for the subjects, may interfere with the objectives of the study, or may suggest an alteration in the absorption, distribution, metabolism or excretion of the drug.
• Having suffered some kind of bone or ligament injury in the last three years.
• History or clinical evidence of psychiatric disorders, alcoholism, drug abuse or addiction to other substances (except for nicotine) or regular consumption of psychoactive drugs. With regard to nicotine, consumers of more than 10 cigarettes/day will be excluded.
• Alcohol consumption over 15 g/day in men and 10 g/day in women.
• Consumers of more than 3 cups of coffee and/or tea per day, consumers of more than 3 units of cola, other stimulant drinks or equivalent per day, in the 2 months prior to the start of the study.
• Regular intake of medication in the month preceding the study. Other kinds of medication may be admitted at the discretion of the Investigator.
• Having donated blood or participated in studies in which there were blood draws in the previous 4 weeks.
• History of allergy, idiosyncrasy, hypersensitivity or adverse reactions to the active principle or any of the excipients. Serious adverse drug reactions.
• Having participated in another clinical trial with medication in the three months prior to the start of the study.
• Subjects with contraindications to treatment with the study drug (according to the summary of product characteristics).
• Positive serology to hepatitis B, C or HIV.
• Being unable to understand the nature, consequences of the trial and the procedures that are asked to follow.

Sponsors & Collaborators

• Parc de Salut Mar: lead

Study Sites (1)

IMIM (Hospital del Mar Medical Research Institute)

Barcelona, 08003, Spain

Location

MeSH Terms

Interventions

Human Growth Hormone

Intervention Hierarchy (Ancestors)

Growth Hormone; Pituitary Hormones, Anterior; Pituitary Hormones; Peptide Hormones; Hormones; Hormones, Hormone Substitutes, and Hormone Antagonists; Peptides; Amino Acids, Peptides, and Proteins

Study Officials

• Ana M Aldea Perona, Dr

  IMIM (Hospital del Mar Medical Research Institute)

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Samples from treatment group are compared to samples from control group (not receiving treatment). It is not possible to use a basal sample of the subjects receiving treatment as a comparator since a minimum period of 2 months' time is required between a 450 mL extraction and another.

Study Record Dates

• First Submitted: July 31, 2019
• First Posted: August 2, 2019
• Study Start: October 25, 2019
• Primary Completion: January 8, 2020
• Study Completion: January 8, 2020
• Last Updated: February 12, 2020

Record last verified: 2019-07

Data Sharing

• IPD Sharing: Will not share

Locations

ES (1)