Evaluation of Possible Genes in Periodontal Diseases by Genetic Methods

NCT04026828 | Unknown

• 1 other identifier: KOU 2019/067
• Study Type: observational
• Target: 200
• Locations: 0 countries
• Sites: N/A

Brief Summary

Generalized aggressive Periodontitis (GAgP) and chronic periodontitis (CP) are inflammatory diseases. Little is known about molecular changes and signaling cascade of host response. Inflammatory diseases are undercontrol of genetic and enviromental factors. Transcription factors are gene-specific factors that are often considered to act as a link connecting genetic and enviromental factors. The aim of this study is to investigate the gene regions that are thought to play a role in the pathogenesis of GAgP and CP, and to interpret new and reliable pathognomonic-prognostic markers in the diagnosis and treatment of these diseases with the help of expression and mutation analyzes and polymorphism studies.

Conditions

Generalized Aggressive Periodontitis; Chronic Periodontitis

Keywords

chronic periodontitis; aggressive periodontitis; expression; polymorphism; mutation

Outcome Measures

Primary Outcomes (3)

• Quality and accuracy of the products of RNA and DNA

  RNA and DNA will be isolated from cells and the quality and accuracy of the products will be tested by Agilent 2100 bioanalyzer chips and quatity of the products will be controlled by Nanodrop ND 1000 spectrophotometer.

  6 months

• Mutations Analysis

  Evaluation will be performed with DNA which extracted from tissues. Amplicons will be reproduced by multiplex PCR, analysed by Ion reporter and the outcomes will be evaluated with diverse online databases and clinical correlations.

  6 Months

• Gene polymorphism

  Genes will evaluated by LightSNiPs

  6 Months

Study Arms (2)

Periodontitis Group

Chronic periodontitis and aggressive periodontitis patients

Genetic: Periodontitis Group

Control Group

Both periodontal and medically healthy volunteers.

Genetic: Periodontitis Group

Interventions

Periodontitis Group GENETIC

Periodontitis group consists of both chronic and aggressive periodontitis patients

Control Group; Periodontitis Group

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Having aggressive or chronic periodontitis and healthy volenteers

You may qualify if:

• The periodontal diagnosis of subjects with GAgP was established on the basis of clinical and radiographic criteria and was defined by the 1999 International World Workshop for a Classification of Periodontal Diseases and Conditions (Lang et al., 1999),
• Between 18 and 35 years of age,
• Otherwise healthy,
• The bone loss estimation was radiographically performed in each patient for the assessment of the extent and severity of alveolar bone loss.
• Had at least 20 teeth,
• Exhibiting \>30% of measured sites with 5mm clinical attachment loss,,
• Had bleeding on probing (BOP) at \>50% of the proximal sites.
• \- Overall healthy individuals (dental, periodontal and systemically)

You may not qualify if:

• Had any known systemic diseases or conditions that can/could influence the periodontal status (cancer, cardiovascular and respiratory diseases),
• Any history of hepatitis and/or HIV infection,
• Immunosuppressive chemotherapy,
• Current pregnancy, planning a pregnancy or lactation,
• Requirement for antibiotic prophylaxis,
• Had oral diseases other than GAgP,
• Oongoing orthodontic therapy,
• A history of antibiotic therapy, or periodontal treatment within the preceding six months

Sponsors & Collaborators

• Kocaeli University: lead

Biospecimen

Retention: SAMPLES WITH DNA

Gingival tissues

MeSH Terms

Conditions

Chronic Periodontitis; Aggressive Periodontitis

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• ESRA GUZELDEMIR-AKCAKANAT, DDS, PhD

  Kocaeli University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

ESRA GUZELDEMIR-AKCAKANAT, DDS, PhD

CONTACT

00905422554664; esragd@yahoo.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Chair of the Department of Periodontology

Study Record Dates

• First Submitted: July 12, 2019
• First Posted: July 19, 2019
• Study Start: September 1, 2019
• Primary Completion: August 31, 2021
• Study Completion: July 15, 2022
• Last Updated: July 25, 2019

Record last verified: 2019-07