PPAR, RXR, and VDR Expressions in Peri-implantitis

NCT04020146 | Completed

• 1 other identifier: Gaziosmanpasa University
• Study Type: observational
• Target: 45
• Locations: 1 country
• Sites: 1

Brief Summary

ABSTRACT Objective: Periodontitis and peri-implantitis are the irreversible destructive diseases of the periodontal and peri-implant tissues. The present study aimed to determine the receptor expressions of Peroxisome proliferative-activated receptor (PPAR)-γ, Retinoid X receptor (RXR)-α, and Vitamin D receptor (VDR) in the diseased tissues around teeth and dental implants. Methods: The study consisted of three groups as group 1; healthy controls (C, n=15), group 2; periodontitis patients with stage 3 grade B (P, n=15), and group 3; peri-implantitis patients (PI, n=15). Periodontal clinical parameters as the plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were recorded. Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H\&E) and immunohistochemistry staining were performed. Total inflammatory cell counts and fibroblast cell density were evaluated on H\&E-stained slides while PPAR-γ, RXR-α, and VDR were evaluated via immunohistochemistry.

Conditions

Periodontitis; Periimplantitis

Keywords

periodontitis; periimplantitis; RXR; VDR

Outcome Measures

Primary Outcomes (5)

• Inflammatory cells counts

  Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H\&E) staining were performed. Total inflammatory cell counts density were evaluated on H\&E-stained slides.A cell counting frame of 10.000 µm2 area was selected under 1000x magnification. The total inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted as inflammatory cell counting.

  6 months

• Fibroblast counts

  Fibroblasts counts were evaluated from H\&E stained slides under 1000x magnification via a light microscope (Nikon Eclipse, E 600, Tokyo, Japan).

  6 months

• Peroxisome proliferative-activated receptor (PPAR)-γ

  Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processin and immunohistochemistry staining were performed, PPAR-γ, was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).

  During histological processing

• Retinoid X receptor (RXR)-α

  Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, RXR-α was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).

  6 months

• Vitamin D receptor (VDR)

  . Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, VDR was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).

  6 months

Study Arms (3)

group 1;

healthy controls (C, n=15),

Other: Gingival biopsies

group 2

periodontitis patients with stage 3 grade B; (P, n=15)

Other: Gingival biopsies

group 3

peri-implantitis patients (PI, n=15).

Other: Gingival biopsies

Interventions

Gingival biopsies OTHER

Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing.

group 1;; group 2; group 3

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

The study consisted of three groups as group 1; healthy controls (C, n=15), group 2; periodontitis patients with stage 3 grade B (P, n=15), and group 3; peri-implantitis patients (PI, n=15).

You may qualify if:

• healthy individuals
• periodontitis patients
• periimplantitis patients

You may not qualify if:

• smokers,
• systemically compromised patients

Sponsors & Collaborators

• Tokat Gaziosmanpasa University: lead

Study Sites (1)

Gaziosmanpasa University

Tokat Province, 60100, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periodontitis; Peri-Implantitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: July 8, 2019
• First Posted: July 15, 2019
• Study Start: January 10, 2018
• Primary Completion: January 10, 2019
• Study Completion: March 10, 2019
• Last Updated: July 15, 2019

Record last verified: 2019-07

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)