Hypotaurine Supplementation Benefits in Cryopreservation

NCT04011813 | Completed

• 1 other identifier: 2014 HYPOTAURINE
• Study Type: observational
• Target: 33
• Locations: 0 countries
• Sites: N/A

Brief Summary

Although it is widely used, slow freezing can induce strong functional and nuclear spermatic alterations reducing the chances of pregnancy. The study objective is to determinate the effects of the combination of hypotaurine supplementation and spermatozoa selection by Density Gradient Centrifugation (DGC) on human sperm functions and DNA quality during a freezing-thawing cycle.

Conditions

Cryopreservation; Human Spermatozoa Parameters; Hypotaurine Supplementation; Spermatozoa DNA Alterations

Keywords

cryopreservation; density gradient centrifugation; DNA fragmentation, oxidation, chromatin packaging; antioxidant hypotaurine supplementation; cryo-capacitation; human spermatozoa fertilizing ability

Outcome Measures

Primary Outcomes (4)

• chromatin packaging labelled using aniline blue and chromomycin A3

  Comparison after a cycle of freezing-thawing for both conditions, with or without hypotaurine supplementation of chromatin packaging labelled using aniline blue and chromomycin A3

  Day 0

• DNA fragmentation using TUNEL assay

  Comparison after a cycle of freezing-thawing for both conditions, with or without hypotaurine supplementation of DNA fragmentation using TUNEL assay

  Day 0

• DNA oxidation assessed by 8-OHdG immunodetections

  Comparison after a cycle of freezing-thawing for both conditions, with or without hypotaurine supplementation of DNA oxidation assessed by 8-OHdG immunodetections

  Day 0

• vacuoles presence in the spermatozoa head using Motile Sperm Organelle Morphology Examination (MSOME)

  Comparison after a cycle of freezing-thawing for both conditions, with or without hypotaurine supplementation of vacuoles presence in the spermatozoa head using Motile Sperm Organelle Morphology Examination (MSOME)

  Day 0

Secondary Outcomes (4)

• vitality using Eosin Nigrosin

  Day 0

• motility total and progressive

  Day 0

• integrity of the acrosome using Fluorescein IsoThioCyanate-Pisum Sativum Agglutinin (FITC-PSA) labelling

  Day 0

• markers of PKA-dependent capacitation intracellular signaling pathway assessing western blot

  Day 0

Study Arms (2)

Control arm, "H-"

semen treated without hypotaurine supplementation in density gradient centrifugation, washing and cryopreservation media

Experimental arm, "H+"

semen treated with a 50mM hypotaurine supplementation in density gradient centrifugation, washing and cryopreservation media

Other: Hypotaurine : antioxidant and osmoregulator

Interventions

Hypotaurine : antioxidant and osmoregulator OTHER

Hypotaurine has protective effects on sperm motility, capacitation and acrosome reaction and reduces apoptotic markers. Hypotaurine (50mM) was added in density gradient centrifugation, washing and cryopreservation media washing and cryopreservation media before spermatozoa freezing

Experimental arm, "H+"

Eligibility Criteria

• Age: 18 Years+
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

Study Population

Men undergoing routine semen analysis at the Center for Reproductive Medicine

You may qualify if:

• None

You may not qualify if:

• None

Sponsors & Collaborators

• University Hospital, Clermont-Ferrand: lead
• Université d'Auvergne, Institut National de la Santéet de la Recherche Médicale: collaborator
• Université d'Auvergne, Centre National de la Recherche Scientifique: collaborator
• University Hospital, Clermont-Ferrand, Hematology laboratory: collaborator

Related Publications (1)

• Pons-Rejraji H, Vorilhon S, Difrane A, Dollet S, Bourgne C, Berger M, Chaput L, Pereira B, Bouche C, Drevet JR, Brugnon F. Beneficial effects of hypotaurine supplementation in preparation and freezing media on human sperm cryo-capacitation and DNA quality. Basic Clin Androl. 2021 Nov 4;31(1):26. doi: 10.1186/s12610-021-00144-6.

  PMID: 34732137 DERIVED

Study Officials

• Florence BRUGNON, MD, PhD

  University Hospital, Clermont-Ferrand

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 21, 2019
• First Posted: July 9, 2019
• Study Start: May 1, 2014
• Primary Completion: August 1, 2018
• Study Completion: May 1, 2019
• Last Updated: July 9, 2019

Record last verified: 2019-06