NCT03954873

Brief Summary

Infusion of glucagon-like peptide 2 (GLP-2) during euglycaemia, hypoglycaemia and hyperglycaemia and evaluation of the effect on glucagon secretion in healthy subjects.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
10

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jan 2019

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 31, 2019

Completed
3 months until next milestone

First Submitted

Initial submission to the registry

May 15, 2019

Completed
2 days until next milestone

First Posted

Study publicly available on registry

May 17, 2019

Completed
1 year until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2020

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2020

Completed
Last Updated

November 4, 2020

Status Verified

November 1, 2020

Enrollment Period

1.3 years

First QC Date

May 15, 2019

Last Update Submit

November 3, 2020

Conditions

Keywords

GLP-2Glucagon

Outcome Measures

Primary Outcomes (1)

  • Glucagon secretion

    Assessed by difference in absolute plasma glucagon concentrations (measured in pmol/liter) and incremental (baseline-subtracted) area under curve (AUC) for plasma glucagon

    110 minutes

Secondary Outcomes (21)

  • Plasma glucose

    110 minutes

  • GLP-1

    110 minutes

  • GLP-2

    110 minutes

  • Insulin

    110 minutes

  • GIP

    110 minutes

  • +16 more secondary outcomes

Study Arms (6)

Hyperglycaemia + GLP-2

EXPERIMENTAL

Glucose + GLP-2

Other: Glucagon-like peptide 2Other: Glucose

Hyperglycaemia + Placebo

ACTIVE COMPARATOR

Glucose + saline

Other: SalineOther: Glucose

Hypoglycaemia + GLP-2

EXPERIMENTAL

Insulin + glucose + GLP-2

Other: Glucagon-like peptide 2Other: GlucoseOther: Insulin

Hypoglycaemia + Saline

ACTIVE COMPARATOR

Insulin + glucose

Other: SalineOther: GlucoseOther: Insulin

Euglycaemia + GLP-2

EXPERIMENTAL

GLP-2

Other: Glucagon-like peptide 2

Euglycaemia + Placebo

ACTIVE COMPARATOR
Other: Saline

Interventions

Human glucagon-like peptide 2

Euglycaemia + GLP-2Hyperglycaemia + GLP-2Hypoglycaemia + GLP-2
SalineOTHER

Placebo

Euglycaemia + PlaceboHyperglycaemia + PlaceboHypoglycaemia + Saline
GlucoseOTHER

20% (w/v) glucose infusion for regulating plasma glucose

Hyperglycaemia + GLP-2Hyperglycaemia + PlaceboHypoglycaemia + GLP-2Hypoglycaemia + Saline
InsulinOTHER

Actrapid (insulin) for lowering plasma glucose

Hypoglycaemia + GLP-2Hypoglycaemia + Saline

Eligibility Criteria

Age18 Years - 40 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Caucasian ethnicity
  • Body mass index (BMI): 18.5-27 kg/m2
  • Glycated haemoglobin (HbA1c) \< 42 mmol/mol
  • Normal haemoglobin (8.3-10.5 mmol/l)
  • Informed and oral and written consent

You may not qualify if:

  • Prediabetes, type 1 diabetes or type 2 diabetes or first-degree relatives with type 1 or type 2 diabetes
  • Nephropathy (eGFR \< 60 and/or albuminuria)
  • Known liver disease and/or alanine transaminase (ALAT) or aspartate transaminase (ASAT) \> 2 × upper normal limit
  • Bilirubin \> 25 μmol/l
  • Known intestinal disease or previous surgery of the intestines
  • Active or recent malignant disease
  • Treatment with medicine that cannot be paused for 12 hours
  • Any condition considered incompatible with participation by the investigators

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Center for Clinical Metabolic Research

Hellerup, Copenhagen, 2900, Denmark

Location

MeSH Terms

Conditions

Glucose Metabolism DisordersMetabolic Diseases

Interventions

Glucagon-Like Peptide 2Sodium ChlorideGlucoseInsulin

Condition Hierarchy (Ancestors)

Nutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Glucagon-Like PeptidesProglucagonGastrointestinal HormonesHormonesHormones, Hormone Substitutes, and Hormone AntagonistsChloridesHydrochloric AcidChlorine CompoundsInorganic ChemicalsSodium CompoundsHexosesMonosaccharidesSugarsCarbohydratesProinsulinInsulinsPancreatic HormonesPeptide HormonesPeptidesAmino Acids, Peptides, and Proteins

Study Officials

  • Filip Krag Knop, MD, PhD

    Center for Clinical Metabolic Research

    STUDY DIRECTOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Clinical Professor

Study Record Dates

First Submitted

May 15, 2019

First Posted

May 17, 2019

Study Start

January 31, 2019

Primary Completion

June 1, 2020

Study Completion

June 1, 2020

Last Updated

November 4, 2020

Record last verified: 2020-11

Data Sharing

IPD Sharing
Will not share

IPD will not be shared with other researchers unless data management agreements are signed.

Locations